Thermo Fisher Scientific Applied Biosystems TaqPath 1-Step Multiplex Master Mix (No ROX)

Thermo Fisher Scientific Applied Biosystems TaqPath 1-Step Multiplex Master Mix (No ROX)

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Product Details:

Storage Instructions Store at -20C; avoid repeated freeze-thaw cycles
Accuracy Highly accurate for multiplex quantitative RNA analysis
Function One-step reverse transcription and quantitative PCR in multiplex format
Instruments Type For use with real-time PCR instruments (without ROX normalization)
Features Sensitive and specific multiplex detection, fast RT, thermostable enzymes, dUTP/UNG contamination control, no ROX reference dye
Measurement Range Wide dynamic range suitable for high and low target copy detection
Shelf Life 12 months from date of manufacture
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Thermo Fisher Scientific Applied Biosystems TaqPath 1-Step Multiplex Master Mix (No ROX) Price And Quantity

Thermo Fisher Scientific Applied Biosystems TaqPath 1-Step Multiplex Master Mix (No ROX) Product Specifications

Color Colorless/clear liquid
Instruments Type For use with real-time PCR instruments (without ROX normalization)
Condition New
Operating Type Automated, compatible with real-time PCR instruments
Weight Not Applicable (reagent format)
Use Multiplex detection of RNA targets in one-tube RT-qPCR reactions (e.g. pathogen detection, gene expression studies)
Measurement Range Wide dynamic range suitable for high and low target copy detection
Real-Time Operation Yes
Storage Instructions Store at -20C; avoid repeated freeze-thaw cycles
Technology 1-Step Real-Time RT-qPCR (Multiplex)
Shelf Life 12 months from date of manufacture
Accuracy Highly accurate for multiplex quantitative RNA analysis
Function One-step reverse transcription and quantitative PCR in multiplex format
Features Sensitive and specific multiplex detection, fast RT, thermostable enzymes, dUTP/UNG contamination control, no ROX reference dye
Usage Type Laboratory, Research, Diagnostic
Wall Mounted No
Material Proprietary enzyme blend and optimized buffer ingredients (contains dNTPs, thermostable MMLV reverse transcriptase, thermostable DNA polymerase)
Portable No (requires laboratory equipment)
Equipment Type Multiplex Real-Time PCR Master Mix
Dimension (L*W*H) Not Applicable (supplied in reagent vial/pack)

Sample Types RNA (purified from samples such as blood, swabs, tissues, etc.)
Contamination Control dUTP and uracil-N-glycosylase (UNG) included for carryover prevention
Product Name Applied Biosystems TaqPath 1-Step Multiplex Master Mix (No ROX)
Compatible Instruments All major real-time PCR systems not requiring ROX normalization
Catalog Number A28527 (may vary by pack size)
ROX Reference Dye Not included (formulation for real-time PCR systems not requiring ROX normalization)
Enzyme Activities Thermostable recombinant MMLV reverse transcriptase, proprietary thermostable DNA polymerase, RNase inhibitor
Reaction Volume Recommended: 1050 L
Format 2X master mix (ready-to-use)
Reaction Time Fast protocol (~1 hour total) for cDNA synthesis and amplification
Temperature Stability Stable for 24 hours at room temperature when thawed
Regulatory Status For Research Use Only. Not for use in diagnostic procedures.
Multiplexing Capacity Up to 4 targets per reaction

Product Description

Brand	Thermo Fisher Scientific
Product Type	RT-qPCR Mix
Pack Size	1 x 0.5 mL
CAT No	A28521
Application	Molecular Biology

TaqPath 1-Step Multiplex Master Mix (No ROX) is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction. Particularly useful for diagnostics and biopharmaceuticals with applications in viral pathogen detection and high throughput gene expression workflows, the mix includes thermostable MMLV reverse transcriptase, dNTPs, UNG, and thermostable Fast DNA polymerase all in a single tube. TaqPath is manufactured under a strong ISO13485 quality management system and is designed to produce consistent product performance lot after lot.

Robust Multiplexing Capability

Applied Biosystems TaqPath 1-Step Multiplex Master Mix (No ROX) is engineered for simultaneous detection of up to four RNA targets within a single reaction, conserving sample and reagents. Its optimized enzyme blend and buffer ensure consistent accuracy and sensitivity across a broad dynamic range, making it an excellent solution for applications such as gene expression studies and pathogen detection in research laboratories.

Optimized for Speed and Contamination Control

The master mix offers a streamlined protocol that delivers cDNA synthesis and amplification in about an hour. Built-in carryover prevention with dUTP and UNG minimizes cross-contamination risks, supporting highly reliable results. The mix maintains stability at room temperature for up to 24 hours after thawing, facilitating hassle-free experimental setup.

Flexible Compatibility and Easy Storage

Suitable for use with all major real-time PCR instruments that do not require ROX normalization, this colorless, clear liquid master mix simplifies sample handling. Its 12-month shelf life at -20C (with instructions to avoid repeated freeze-thaw cycles) ensures long-term usability and consistent performance in laboratory and research environments.

FAQ's of Thermo Fisher Scientific Applied Biosystems TaqPath 1-Step Multiplex Master Mix (No ROX):

Q: How is the Applied Biosystems TaqPath 1-Step Multiplex Master Mix (No ROX) used in molecular research?

A: This master mix is employed for multiplex one-step reverse transcription and real-time quantitative PCR (RT-qPCR) analysis of RNA, allowing users to detect and quantify up to four RNA targets from samples such as blood, swabs, or tissues in a single tube.

Q: What are the benefits of using a master mix without ROX reference dye?

A: The no-ROX master mix is ideal for real-time PCR instruments that do not require ROX normalization, eliminating unnecessary components and ensuring compatibility while simplifying assay setup for such platforms.

Q: When is contamination control necessary during RT-qPCR and how does this mix provide it?

A: Contamination control is crucial to prevent false positives due to carryover from previous PCR products. This master mix incorporates dUTP and uracil-N-glycosylase (UNG), which actively prevent amplification of residual DNA contaminants from previous reactions.

Q: Where can this master mix be stored, and what are the storage requirements?

A: The master mix should be stored at -20C. It is important to avoid repeated freeze-thaw cycles to maintain its activity and effectiveness. Once thawed, it remains stable at room temperature for up to 24 hours during experimental setup.

Q: What sample types are compatible with this master mix?

A: It can be used with purified RNA from a variety of sources, including blood, swabs, tissues, and other biological materials, supporting broad research applications.

Q: What instruments are compatible with this product?

A: The mix is compatible with all major real-time PCR systems that do not require ROX dye for signal normalization, offering flexibility for different laboratory setups.

Q: How does this master mix enhance laboratory workflows?

A: Its ready-to-use formulation, fast one-step protocol, robust contamination control, and multiplexing capability streamline experimental procedures, reduce hands-on time, and promote accurate, reproducible results in molecular research.

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