Thermo Fisher Scientific Applied Biosystems TaqMan Genotyping Master Mix

Thermo Fisher Scientific Applied Biosystems TaqMan Genotyping Master Mix

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Product Details:

Accuracy High genotyping accuracy (assay-dependent)
Measurement Range Assay-dependent
Usage Type Laboratory
Features Hot-start DNA polymerase, robust performance, optimized for TaqMan Genotyping Assays, compatibility with Applied Biosystems instruments
Instruments Type qPCR thermal cyclers
Function Supports allelic discrimination and SNP genotyping in qPCR
Shelf Life 12 months (when properly stored)
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Thermo Fisher Scientific Applied Biosystems TaqMan Genotyping Master Mix Price And Quantity

Thermo Fisher Scientific Applied Biosystems TaqMan Genotyping Master Mix Product Specifications

Weight Not Applicable (packaged in bottles/tubes)
Color Clear/Colorless liquid
Material Solution / Reagent mix (proprietary chemical formulation)
Use Genotyping analysis
Usage Type Laboratory
Equipment Type Genotyping Master Mix
Condition New
Shelf Life 12 months (when properly stored)
Instruments Type qPCR thermal cyclers
Features Hot-start DNA polymerase, robust performance, optimized for TaqMan Genotyping Assays, compatibility with Applied Biosystems instruments
Function Supports allelic discrimination and SNP genotyping in qPCR
Operating Type Automatic (for qPCR instruments)
Accuracy High genotyping accuracy (assay-dependent)
Storage Instructions Store at -20C
Real-Time Operation Yes
Wall Mounted No
Dimension (L*W*H) Not Applicable (provided in tube/bottle format)
Measurement Range Assay-dependent
Portable Yes (reagent format)
Technology TaqMan Real-Time PCR (qPCR)

Buffer System Optimized buffer with dNTPs and MgCl2
Package Size Available in 1 mL, 5 mL, and custom pack sizes
Formulation 2X master mix (pre-mixed for ease of use)
Reaction Volume Range Up to 50 µL per reaction
Catalog Number 4371355 (multiple sizes available)
Compatibility Validated for use with TaqMan Genotyping Assays and Applied Biosystems real-time PCR systems
Application SNP genotyping, allelic discrimination, real-time PCR
Enzyme Hot-start DNA polymerase

Product Description

Brand	Thermo Fisher Scientific
Product Type	Master Mix
Pack Size	2 x 50 mL
CAT No	4381657
Application	Molecular Biology

TaqMan Genotyping Master Mix is optimized for end-point fluorescence detection in SNP genotyping applications.

a Distinct clusters and high call rates for unambiguous allelic discrimination
a Validated with TaqMana SNP Genotyping Assays
a Excellent pre- and post-PCR stability for high throughput setup and analysis.

Optimized Formulation for Peak Performance

The 2X TaqMan Genotyping Master Mix is specifically crafted for seamless integration with TaqMan Genotyping Assays and Applied Biosystems qPCR instruments. Its proprietary buffer system, including dNTPs and MgCl2, and the inclusion of a hot-start DNA polymerase, enhance assay specificity and signal clarity, supporting highly accurate SNP genotyping results.

Versatile Applications in Genotyping Analysis

This robust master mix is ideal for allelic discrimination and SNP genotyping, offering consistent results in real-time PCR workflows. It is suitable for use in research, clinical, or industrial laboratories, catering to various throughput requirements with available pack sizes and compatibility with multiple Applied Biosystems real-time PCR platforms.

User-Friendly and Reliable Workflow

Delivered as a clear, colorless solution, the pre-mixed master mix streamlines sample preparation-simply add your assay and template DNA. The reagent remains stable for 12 months at -20C, supporting scheduled and ongoing project needs while minimizing retesting and reagent waste.

FAQ's of Thermo Fisher Scientific Applied Biosystems TaqMan Genotyping Master Mix:

Q: How do I use the TaqMan Genotyping Master Mix in my workflow?

A: Simply combine the 2X master mix with your TaqMan Genotyping Assay and DNA samples, then load the mixture into your Applied Biosystems real-time PCR instrument for SNP genotyping or allelic discrimination. The pre-mixed formulation minimizes pipetting steps and potential errors.

Q: What are the main benefits of using this master mix for qPCR genotyping?

A: The master mix offers high genotyping accuracy, improved specificity due to the hot-start DNA polymerase, and robust performance for allelic discrimination. Its optimized formulation supports reliable results and streamlined workflow, saving both time and resources in the laboratory.

Q: When should I order larger pack sizes or custom packaging?

A: Larger or custom pack sizes are recommended if you perform high-throughput or routine genotyping analyses or require bulk supply for extended projects. This ensures you have sufficient reagent and reduces the frequency of reordering, supporting uninterrupted laboratory processes.

Q: What storage conditions are required to maintain product stability?

A: The master mix should be stored at -20C to ensure its 12-month shelf life and preserve reagent integrity. Avoid repeated freeze-thaw cycles and keep it protected from light by storing in its original container until use.

Q: Which instruments are compatible with this genotyping master mix?

A: The TaqMan Genotyping Master Mix is validated for optimal performance with Applied Biosystems real-time PCR systems and other compatible qPCR thermal cyclers, ensuring seamless use in most laboratory setups focused on genotyping.

Q: Where is this product commonly used and who are the typical users?

A: This master mix is widely used by researchers, laboratory scientists, and technicians in academic, clinical, and industrial genetics laboratories, particularly for SNP genotyping and allelic discrimination applications where high accuracy and reproducibility are critical.

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