Product Description
Brand Qiagen
Product Type RNA Kit
Pack Size 250T
CAT No 74536
Application Molecular Reagent
The QIAwave RNA Mini Kit is an eco-friendlier version of our standard RNeasy Mini Kit. The kit uses up to 60% less plastic and up to 57% less cardboard than our standard kit and offers waste tubes made from 100% post-consumer recycled plastic that you can reuse throughout the procedure. QIAwave buffers also come as concentrates, reducing the amount of plastic by up to 90% per bottle. To save paper, there are no printed protocols in the kit. Instead, you can download the protocols you need from the resources list or by simply scanning the QR code inside the box lid. So, while the kit packaging and components of our QIAwave Kit may look different, its as easy to use as the RNeasy Mini Kit, and the chemistry and performance are identical.
Efficient RNA Extraction for Diverse Sample TypesThe QIAwave RNA Mini Kit is engineered for rapid and effective RNA extraction from animal and human tissues, cultured cells, and bacteria. Its robust, silica-membrane technology ensures high yields and purity, meeting the stringent requirements of modern genomics laboratories. With easy-to-use mini-spin columns and RNase-free reagents, users can consistently isolate RNA ready for sensitive downstream applications.
Streamlined Protocol with High Yield and PurityProcessing each sample takes approximately 20 minutes, providing researchers with up to 100 g total RNA per column and overall high accuracy. The kit is suitable for a measurement range of 1-10^7 cells or up to 30 mg of tissue per preparation, making it highly adaptable to both small- and large-scale studies. Its RNase-free guarantee and user-friendly format promote reliable and reproducible results.
Ready-to-Use Components for Laboratory ConvenienceEach QIAwave RNA Mini Kit includes individually packaged spin columns, collection tubes, proprietary buffers, and a detailed protocol booklet. The kit is designed for manual operation, making it portable and versatile across different laboratory environments. All components are housed in color-coded, clearly labeled packaging for ease of identification during use.
FAQ's of Qiagen QIAwave RNA Mini Kit (250):
Q: How does the QIAwave RNA Mini Kit isolate RNA from cells and tissues?
A: The kit utilizes a silica membrane spin column technology that selectively binds RNA while impurities are washed away. Following lysis and homogenization of the sample, RNA is captured on the column, washed with included buffers, and then eluted in RNase-free water, providing high-quality RNA suitable for sensitive applications.
Q: What types of samples can I process with this kit?
A: You can use the QIAwave RNA Mini Kit to extract total RNA from animal tissues, human tissues, cultured cells, and bacteria. This versatility makes it ideal for a wide range of biological and clinical research applications.
Q: When is it appropriate to use the QIAwave RNA Mini Kit in the lab?
A: The kit is best used whenever you require high-quality RNA for downstream processes such as RT-PCR, gene expression analysis, Northern blotting, or microarray assays. It is suitable for both routine and advanced research purposes.
Q: Where should I store the QIAwave RNA Mini Kit to maintain its effectiveness?
A: To ensure maximum shelf life and performance (typically 12-24 months), store the kit at room temperature between 15-25C and in an RNase-free environment. Always refer to the individual packaging for specific storage instructions.
Q: What is the typical process and time required for RNA extraction with this kit?
A: The protocol is straightforward and manual, allowing RNA purification in about 20 minutes per sample. Simply lyse your sample, transfer to the spin column, perform the washing steps, and elute the RNA using the provided buffers and columns.
Q: How much RNA can I expect to extract per column and how accurate are the results?
A: Each mini spin column has a binding capacity of up to 100 g total RNA, with typical yields reaching up to 45 g, depending on the sample type and input amount. The technology ensures high accuracy and purity suitable for sensitive downstream applications.
Q: What are the main benefits of using the QIAwave RNA Mini Kit in RNA analysis workflows?
A: This kit delivers rapid, reliable, and RNase-free purification of high-yield RNA. Its easy-to-use design, comprehensive components, and compatibility with various sample types make it an essential tool for obtaining high-quality results in genomics, diagnostics, and molecular biology research.