Qiagen QuantiNova Probe PCR Kit

Get a Price/Quote

Product Details:

Features High sensitivity and specificity, fast cycling, easy workflow, compatible with various probe formats
Measurement Range Wide dynamic range for target quantification
Accuracy High, suitable for quantitative analysis
Storage Instructions Store at -15C to -30C
Function Amplification and quantification of nucleic acids
Usage Type Laboratory use
Instruments Type PCR Amplification Reagents
Click to View more
Share Your Product:

Request to Call Back Send Inquiry

Qiagen QuantiNova Probe PCR Kit Price And Quantity

Qiagen QuantiNova Probe PCR Kit Product Specifications

Instruments Type PCR Amplification Reagents
Portable Yes, as a kit
Usage Type Laboratory use
Condition New
Real-Time Operation Yes
Technology qPCR (quantitative polymerase chain reaction) probe-based detection
Storage Instructions Store at -15C to -30C
Function Amplification and quantification of nucleic acids
Shelf Life At least 12 months (if stored properly)
Measurement Range Wide dynamic range for target quantification
Wall Mounted No
Equipment Type PCR Kit
Material Reagents for molecular biology
Operating Type Manual, compatible with real-time PCR systems
Features High sensitivity and specificity, fast cycling, easy workflow, compatible with various probe formats
Accuracy High, suitable for quantitative analysis
Use Quantitative gene expression analysis, DNA/RNA detection
Color Clear or colorless reagents

Product Description

Brand Qiagen

Product Type PCR Kit

Pack Size 100, 2500T

CAT No 208252, 208256

Application Molecular Reagent

Featuring enhanced specificity, sensitivity, speed, and process safety, the QuantiNova SYBR Green PCR Kit (SYBR qPCR kit) sets new standards in SYBR Green-based qPCR. A novel hot-start mechanism significantly increases the specificity of real-time PCR and optimized chemistry produces accurate quantitative results from cDNA or gDNA on any real-time PCR cycler with detection of even a single target copy. Finally, a built-in visual indicator ensures correct pipetting.

High Sensitivity and Specificity for qPCR

The QuantiNova Probe PCR Kit utilizes advanced Hot-starTaq Plus hot-start DNA Polymerase and an optimized master mix to ensure precise and reliable amplification. These components, together with optional ROX reference dye, enable accurate quantification of your DNA and RNA targets, even at low template concentrations.

Universal Compatibility and Easy Workflow

The kit is universally compatible with all probe-based real-time PCR instruments, making setup and integration straightforward. It provides clear, colorless reagents, and comes with all necessary components including dilution buffer and detailed instructions, streamlining your laboratory workflow for both routine and specialized applications.

Long Shelf Life and Stable Storage

Designed for durability, the QuantiNova Probe PCR Kit maintains its high performance for at least 12 months when stored at -15C to -30C. This optimal storage condition preserves reagent quality, ensuring consistent results for your molecular biology experiments.

FAQs of Qiagen QuantiNova Probe PCR Kit:

Q: How do I set up a reaction using the Qiagen QuantiNova Probe PCR Kit?

A: To set up a reaction, combine the QuantiNova Probe PCR Master Mix, your primers and probe, template DNA or cDNA, RNase-free water, and the optional ROX reference dye as required for your real-time PCR instrument. The kits protocol, included in the handbook, details each step for accurate and reliable results.

Q: What types of samples are suitable for this PCR kit?

A: The QuantiNova Probe PCR Kit is compatible with cDNA, gDNA, and RNA samples (after reverse transcription). This flexibility supports various applications, from gene expression analysis to DNA/RNA detection in molecular biology laboratories.

Q: When should I use the ROX reference dye included in the kit?

A: Use the ROX reference dye when your real-time PCR instrument requires passive reference normalization. Since it is provided in a separate vial, you can easily add the dye according to the specifications of your particular PCR platform.

Q: Where should I store the kit and its components for optimal performance?

A: Store all components of the QuantiNova Probe PCR Kit at temperatures between -15C and -30C. Maintaining these conditions ensures reagent stability and the longevity of the kit, with a guaranteed shelf life of at least 12 months.

Q: What are the benefits of using the Hot-starTaq Plus polymerase in this kit?

A: Hot-starTaq Plus polymerase offers hot-start activation, which minimizes non-specific amplification and increases assay specificity and sensitivity. This feature is particularly beneficial for achieving reliable and reproducible qPCR results.

Q: How does this kit ensure high accuracy in quantitative analysis?

A: The optimized master mix, stringent buffer conditions, and hot-start enzyme reduce background noise and improve the precision of target quantification. The kit supports a broad dynamic range, making it ideal for accurate quantitative analysis across various template concentrations.

Q: Can this kit be used with all real-time PCR instruments?

A: Yes, the QuantiNova Probe PCR Kit is universally compatible with all probe-based real-time PCR systems, accommodating different probe formats and instrument requirements for versatile laboratory use.

Tell us about your requirement

Price:

Quantity

Select Unit

50
100
200
250
500
1000+

Additional detail

Mobile number

Other Products in 'Medical Reagents' category

Mindray Anti-mullerian Hormone (CLIA) Color : White/transparent cartridge, colorless reagents Material : Other, Diagnostic reagents, plastic cartridge Features : High sensitivity and specificity, rapid results, wide measurement range Wall Mounted : No Measurement Range : 0.0123.00 ng/mL (varies by lot) Shelf Life : 12 months from date of manufacture (refer to packaging) Function : Measurement of Antimullerian Hormone for assessment of ovarian reserve and fertility status Real-Time Operation : Yes Technology : Chemiluminescence Immunoassay (CLIA) Condition : Other, New and sterile Accuracy : High (correlated to International AMH Reference) Use : Quantitative detection of Antimullerian Hormone (AMH) in human serum or plasma Operating Type : Other, Automated Equipment Type : Chemiluminescence Immunoassay (CLIA) Kit Storage Instructions : Store at 28C, protect from light Instruments Type : Compatible only with Mindray CLseries analyzers Portable : No (designed for laboratory analyzers) Usage Type : Diagnostic laboratory use Dimension (L*W*H) : Cartridge dimensions: Standard for Mindray CLIA systems Weight : Lightweight (packaged, varies by kit size)

Send Inquiry

Thermo Fisher Scientific Applied Biosystems TaqPath 1-Step Multiplex Master Mix (No ROX) Color : Colorless/clear liquid Material : Other, Proprietary enzyme blend and optimized buffer ingredients (contains dNTPs, thermostable MMLV reverse transcriptase, thermostable DNA polymerase) Features : Sensitive and specific multiplex detection, fast RT, thermostable enzymes, dUTP/UNG contamination control, no ROX reference dye Wall Mounted : No Measurement Range : Wide dynamic range suitable for high and low target copy detection Shelf Life : 12 months from date of manufacture Function : Onestep reverse transcription and quantitative PCR in multiplex format Real-Time Operation : Yes Technology : 1Step RealTime RTqPCR (Multiplex) Condition : New Accuracy : Highly accurate for multiplex quantitative RNA analysis Use : Multiplex detection of RNA targets in onetube RTqPCR reactions (e.g. pathogen detection, gene expression studies) Operating Type : Other, Automated, compatible with realtime PCR instruments Equipment Type : Multiplex RealTime PCR Master Mix Storage Instructions : Store at 20C; avoid repeated freezethaw cycles Instruments Type : For use with realtime PCR instruments (without ROX normalization) Portable : No (requires laboratory equipment) Usage Type : Laboratory, Research, Diagnostic Dimension (L*W*H) : Not Applicable (supplied in reagent vial/pack) Weight : Not Applicable (reagent format)

Send Inquiry

QIAGEN Plasmid Plus Maxi Kit (100) Color : White/Blue Material : Other, Highquality plastic components, prefilled buffer tubes Features : Rapid purification, high yield, high purity, scalable Wall Mounted : No Measurement Range : For 150750 ml E. coli culture per prep Shelf Life : 12 months Function : Plasmid DNA extraction and purification Real-Time Operation : No Technology : Anionexchange silica membrane technology Condition : New Accuracy : High quality DNA yield (A260/A280 ratio: 1.801.90) Use : Isolation and purification of plasmid DNA from bacterial cultures Operating Type : Manual Equipment Type : Plasmid DNA Purification Kit Storage Instructions : Store at room temperature (1525C) Instruments Type : Life Sciences Research Kit Portable : Yes Usage Type : Laboratory Use

Send Inquiry

Thermo Fisher Scientific Applied Biosystems TaqPath ProAmp Master Mix Color : Clear/Colorless Material : Other, Proprietary enzyme blend, buffer solution Features : High specificity, robust performance, compatibility with fast cycling protocols, reduced PCR inhibition Wall Mounted : No Measurement Range : Dynamic range across multiple log dilutions of target DNA Shelf Life : 12 months Function : Enables amplification of DNA samples for analysis Real-Time Operation : Yes Technology : qPCR (realtime quantitative PCR) Condition : New Accuracy : High amplification fidelity Use : DNA amplification, genotyping, gene expression analysis Operating Type : Other, Manual/Pipette loading Equipment Type : PCR Master Mix Storage Instructions : Store at 20C Instruments Type : Designed for use with compatible qPCR instruments Portable : Yes, reagent tube form Usage Type : Laboratory

Send Inquiry

SEND INQUIRY CALL ME FREE