Applied Biosystems AmpFLSTR Identifiler Plus PCR Amplification Kit

Get a Price/Quote

Product Details:

Measurement Range 50500 ng DNA input recommended
Instruments Type PCR kit (for use with genetic analyzers)
Features Amplifies 15 autosomal STR loci and Amelogenin; robust performance; short set-up time
Accuracy High sensitivity (low template DNA capability)
Function Multiplex amplification of STR markers for DNA typing
Usage Type Laboratory diagnostic
Shelf Life 12 months from manufacturing date
Click to View more
Share Your Product:

Request to Call Back Send Inquiry

Applied Biosystems AmpFLSTR Identifiler Plus PCR Amplification Kit Price And Quantity

Maximum Samples per Kit 200 reactions (standard kit size)
PCR Reaction Volume 25 µL per reaction
Detection Platform capillary electrophoresis on Applied Biosystems genetic analyzers
Thermal Cycler Compatibility Compatible with most standard PCR thermal cyclers
Kit Components Primer set, PCR reaction mix, control DNA, allelic ladder
Product Code Applied Biosystems 4440553 (kit size may vary)
Number of Loci Detected 15 STR loci plus Amelogenin (gender determination)
Sample Types Buccal swabs, blood, forensic samples
Application Forensic DNA typing, paternity testing, population genetics research

Applied Biosystems AmpFLSTR Identifiler Plus PCR Amplification Kit Product Specifications

Accuracy High sensitivity (low template DNA capability)
Equipment Type PCR Amplification Kit
Material Biological reagents, enzyme mix, buffer solutions
Instruments Type PCR kit (for use with genetic analyzers)
Features Amplifies 15 autosomal STR loci and Amelogenin; robust performance; short set-up time
Power Source Not required (used in standard thermal cycler)
Operating Type Manual (laboratory-based)
Technology Multiplex PCR (Polymerase Chain Reaction)
Function Multiplex amplification of STR markers for DNA typing
Wall Mounted No
Condition New
Noise Level Silent operation
Usage Type Laboratory diagnostic
Color Clear/Colorless reagents in labeled vials
Portable No
Measurement Range 50500 ng DNA input recommended
Shelf Life 12 months from manufacturing date
Use DNA profiling, forensic and human identification
Real-Time Operation No
Storage Instructions Store at -15C to -25C

Maximum Samples per Kit 200 reactions (standard kit size)
PCR Reaction Volume 25 µL per reaction
Detection Platform capillary electrophoresis on Applied Biosystems genetic analyzers
Thermal Cycler Compatibility Compatible with most standard PCR thermal cyclers
Kit Components Primer set, PCR reaction mix, control DNA, allelic ladder
Product Code Applied Biosystems 4440553 (kit size may vary)
Number of Loci Detected 15 STR loci plus Amelogenin (gender determination)
Sample Types Buccal swabs, blood, forensic samples
Application Forensic DNA typing, paternity testing, population genetics research

Product Description

Brand	Thermo Fisher Scientific
Product Type	PCR Amplification Kit
Pack Size	200T
CAT No	4427368
Application	Molecular Biology

AmpFLSTR Identifiler Plus PCR Amplification Kit enables forensic analysts to recover more interpretable results from a wider range of DNA evidence samples with increased confidence. The new kit includes the same primers and allelic ladder as the widely used Identifiler Kit and harnesses next-generation PCR amplification technology to help provide enhanced sensitivity, a cleaner baseline, improved performance on mixtures, and the ability to overcome high levels of PCR inhibition.

Efficient Multiplex PCR for DNA Profiling

The Identifiler Plus kit streamlines DNA typing by allowing simultaneous amplification of 15 STR loci and Amelogenin in a single reaction. Its advanced formulation ensures reliable results even with challenging forensic and low-quantity samples. Compatible with most thermal cyclers and designed for use on Applied Biosystems capillary electrophoresis platforms, this kit is a trusted choice for laboratories conducting forensic human identification and research studies.

Comprehensive Kit Components and Coverage

This PCR kit includes a primer set, PCR reaction mix, control DNA, and an allelic ladder-everything needed for successful DNA amplification. It is optimized for a measurement range of 50-500 ng DNA input and accommodates various human sample types, including buccal swabs and blood. Each kit supports up to 200 reactions and is supplied with clear, labeled vials for easy identification and handling in the lab.

Reliable and Convenient Laboratory Solution

With silent operation and a straightforward manual protocol, the Identifiler Plus kit ensures ease of use in laboratory settings. The reagents are colorless, require no special power source, and must be stored between -15C and -25C to maintain performance throughout their 12-month shelf life. This makes the kit an ideal, low-maintenance solution for consistent, high-accuracy DNA profiling.

FAQ's of Applied Biosystems AmpFLSTR Identifiler Plus PCR Amplification Kit:

Q: How does the Applied Biosystems AmpFLSTR Identifiler Plus PCR Amplification Kit benefit forensic DNA analysis?

A: This kit enables simultaneous amplification of 15 STR loci and Amelogenin, providing high-resolution genetic profiles for forensic human identification, paternity cases, and population genetics research. Its robust performance ensures reliable results even from low-input or degraded DNA samples.

Q: What sample types can be used with this PCR amplification kit?

A: The kit is validated for use with buccal swabs, blood samples, and a broad range of forensic specimens, making it versatile and adaptable for various laboratory applications.

Q: When should the kit components be used, and how should they be stored?

A: The reagents should be used within 12 months from the manufacturing date and must be stored at temperatures between -15C and -25C to ensure optimal performance and stability throughout their shelf life.

Q: Where are the results detected and analyzed after PCR amplification?

A: After PCR amplification, the STR fragments are separated and detected via capillary electrophoresis using Applied Biosystems genetic analyzers, allowing precise DNA typing and identification.

Q: What is the maximum number of samples that can be processed per standard kit?

A: A standard kit can process up to 200 samples, based on a 25 L PCR reaction volume for each reaction, making it suitable for medium to high-throughput laboratory workflows.

Q: How is the kit used in laboratory settings, and what equipment is required?

A: The kit is intended for manual use in laboratories. Reactions are set up in PCR tubes or plates, run on standard thermal cyclers, and require downstream analysis on Applied Biosystems capillary electrophoresis instruments. No special power source or wall mounting is needed, ensuring flexibility in laboratory environments.

Q: What distinguishes this kit from other DNA amplification kits?

A: Its ability to amplify 15 autosomal STR loci plus Amelogenin in a single multiplex reaction, combined with high sensitivity and short setup times, makes it exceptionally efficient for laboratory diagnostic and forensic applications.

Tell us about your requirement

Price:

Quantity

Select Unit

50
100
200
250
500
1000+

Additional detail

Mobile number

Other Products in 'Medical Reagents' category

Cepheid Xpert MTB/XDR Material : Other, Plastic Cartridge, Electronic Module Operating Type : Automatic Technology : Molecular Diagnostic (PCR) Technology Storage Instructions : 228C (Cartridge); avoid freezing Usage Type : Clinical, Laboratory diagnostic Shelf Life : 12 months (Cartridge) Function : Detection of MTB, MultiDrug Resistance (MDR), XDR markers Wall Mounted : No Instruments Type : Molecular Diagnostic Assay Cartridge Features : Automated nucleic acid extraction, Amplification, Detection, Sampletoresult platform, Minimal handson time Use : Detection of MTB (Mycobacterium tuberculosis) and drug resistance (INH, FQ, ETH, AMK, KAN, CAP) Condition : New Portable : Yes, with certain instrument configurations Accuracy : High (Factoryvalidated with controls included per run) Color : White/Blue (Cartridge color: Translucent/White) Equipment Type : Real Time PCR System Cartridge Real-Time Operation : Yes Measurement Range : Qualitative detection of MTB and resistance mutations Power Source : Electric Weight : Varies with instrument module; e.g., 13 kg (GeneXpert System) Dimension (L*W*H) : Varies by instrument platform; e.g., 32.5 cm x 35 cm x 28 cm (GeneXpert instrument module) Frequency : 5060 Hz Noise Level : Low

Send Inquiry

Erba Mannheim System Pack Iron - FE 125 Kit Material : Other, Reagent Solution (Liquid) Operating Type : Other, Automated Technology : Colorimetric Storage Instructions : Store at 28C, Keep away from sunlight Usage Type : Laboratory Use Shelf Life : 18 months from manufacturing Function : Quantitative Determination of Iron in Serum Wall Mounted : No Instruments Type : Biochemistry Analyzer Reagent Features : Ready to use, For Clinical Chemistry, Long Shelf Life Use : InVitro Diagnostic Assay for Determination of Serum Iron Condition : New Portable : Yes Accuracy : High, as per clinical standards Color : Colorless to Light Yellow Solution Equipment Type : Iron (FE) System Pack Kit Real-Time Operation : Yes Measurement Range : Up to 1000 g/dL Power Source : Other, Not Required (used with Biochemistry Autoanalyzer) Weight : Approx. 180 g Dimension (L*W*H) : 125 ml pack Frequency : As per analyzer requirement Noise Level : Silent

Send Inquiry

Thermo Fisher Scientific Invitrogen Platinum SYBR Green qPCR SuperMix-UDG Material : Other, Liquid reagent; primarily buffer and enzyme solution Operating Type : Other, Readytouse master mix for realtime PCR Technology : RealTime PCR with SYBR Green I dye detection Storage Instructions : Store at 20 C for longterm stability Usage Type : Laboratory/Research Shelf Life : 12 months (when stored properly) Function : Enables sensitive DNA or cDNA quantification via realtime PCR Wall Mounted : No Instruments Type : Compatible with most realtime PCR instruments Features : Contains Platinum Taq DNA Polymerase, SYBR Green I dye for doublestranded DNA detection, UDG (uracilDNA glycosylase) for prevention of carryover contamination, optimized buffer system, high sensitivity Use : Quantitative PCR (qPCR) and quantitative reversetranscription PCR (qRTPCR) for DNA and cDNA target detection Condition : New Portable : Yes (reagent format) Accuracy : High sensitivity and specificity for qPCR applications Color : Light green (due to SYBR Green dye) Equipment Type : qPCR SuperMix Real-Time Operation : Yes Measurement Range : Dynamic range typically: 68 orders of magnitude (depending on instrument and assay) Power Source : Other, Not required (used as a reagent in compatible qPCR cyclers) Dimension (L*W*H) : Not applicable (reagent bottle/tube format)

Send Inquiry

Erba Mannheim LIQUIXX Triglycerides Kit Material : Other, Liquid Stable Reagents Operating Type : Other, Manual or Automated Analyzer Compatible Technology : GPOPAP Enzymatic Colorimetric Method Storage Instructions : Store at 28C, Do not Freeze Usage Type : Laboratory Use Shelf Life : 24 Months Function : Biochemical Analysis of Triglycerides in Human Serum and Plasma Wall Mounted : No Instruments Type : Clinical Chemistry Analyzer Features : Liquid Stable, Readytouse, Long Shelflife, High Accuracy Use : Quantitative Determination of Serum/Plasma Triglycerides Condition : New Portable : Yes Accuracy : Within Specified Range as Provided by Kit Insert Color : Clear/Colorless to Slightly Yellow Liquid Equipment Type : InVitro Diagnostic Reagent Kit Real-Time Operation : No Measurement Range : Up to 1000 mg/dL (varies based on analyzer)

Send Inquiry

SEND INQUIRY CALL ME FREE