Product Description
Brand Qiagen
Product Type PCR Kits
Pack Size 480 T, 5 x 96 Wells
CAT No 51531
Application Molecular Reagent
The QIAamp 96 PowerFecal QIAcube HT Kit provides simple, automated purification of high-quality genomic DNA (human and bacterial) from up to 200 mg fresh or frozen stool samples using theQIAcube HT. The novel combination of MOBIOs patented Inhibitor Removal Technology (IRT) and proven QIAamp purification system provides an optimized DNA purification solution for microbiome, infectious disease and metabolic research applications.
Efficient DNA Purification from Challenging SamplesThe QIAamp 96 PowerFecal QIAcube HT Kit is engineered for extracting DNA from complex stool and fecal samples. By integrating inhibitor removal technology with high-throughput automation, this kit maximizes DNA yield and purity even from difficult matrices. Its compatibility with advanced robotic systems ensures repeatable and consistent results, making it ideal for research, clinical labs, and diagnostics.
Streamlined Automation with the QIAcube HT SystemOptimized for the QIAcube HT platform and other liquid handling instruments, this kit delivers automated processing for up to 96 samples per run. The included 96-well plates, buffers, and reagents facilitate hassle-free setup and reproducibility. Automation minimizes manual intervention, reducing the risk of cross-contamination and allowing laboratory staff to focus on higher-value tasks.
FAQs of Qiagen QIAamp 96 PowerFecal QIAcube HT Kit (5):
Q: How does the QIAamp 96 PowerFecal QIAcube HT Kit process samples for DNA extraction?
A: Samples are loaded into a 96-well format and processed using an automated workflow. PowerBead mechanical lysis disrupts tough stool matrices, and silica-membrane technology binds DNA while inhibitors are removed, resulting in high-purity DNA suitable for downstream applications.
Q: What types of samples are compatible with this kit?
A: The kit is specifically designed for stool and fecal sample matrices. It accommodates up to 250 mg stool or 250 l stool suspension per well, ensuring broad applicability for human and animal microbiome studies.
Q: When should I use this kit for my lab workflow?
A: This kit is ideal for high-throughput DNA isolation needs, especially when processing large numbers of fecal or stool samples for PCR, qPCR, or sequencing. Its efficiency streamlines workflows and reduces processing time per sample batch.
Q: Where can the QIAamp 96 PowerFecal QIAcube HT Kit be used?
A: It is intended for laboratory environments equipped with compatible automation, such as the Qiagen QIAcube HT or QIAsymphony SP systems. It can be used by research, clinical, or diagnostic labs requiring reliable DNA extraction from fecal samples.
Q: What is the process time for a complete run?
A: The entire process for 96 samples can be completed in approximately 2 hours, making it suitable for labs with stringent throughput and time requirements.
Q: How should this kit be stored and what is its shelf life?
A: Store the kit at room temperature (1525C) as directed on the label. Typically, the shelf life is at least 12 months from the manufacturing date, but always check your kits specific expiration information.
Q: What are the benefits of using this kit for DNA isolation from stool samples?
A: The kit provides high DNA yield and purity, reliable removal of PCR inhibitors, and compatibility with automated liquid handling systems. It enables efficient and reproducible results, reduces manual labor, and supports a wide range of molecular biology applications.