Product Description
Brand Qiagen
Product Type PCR Kits
Pack Size 96 Wells
CAT No 59971
Application Molecular Reagent
Hi-C technology is a powerful technique for genome-wide chromosome conformation capture that enables the characterization of chromatin folding at kb resolution. The EpiTect Hi-C Kit offers a robust, yet simple and fast, protocol with low cell input requirements that enables generation of high-quality Hi-C Illumina NGS libraries from cross-linked cells in less than 2 days.
Enabling 3D Genome Organization AnalysisThe EpiTect Hi-C Kit (6) empowers researchers to study the intricate architecture of the genome by mapping chromatin interactions on a genome-wide scale. Its optimized components and workflow make exploring nuclear organization straightforward and data-rich, facilitating insights into gene regulation and epigenetic landscapes.
Streamlined Workflow for High ReproducibilityThis kit stands out for its high efficiency and reproducibility, supporting a robust manual workflow that can typically be completed within 1-2 days. Compatibility with major NGS platforms ensures that researchers can seamlessly integrate library preparation with their sequencing operations.
FAQ's of Qiagen EpiTect Hi-C Kit (6):
Q: How is the Qiagen EpiTect Hi-C Kit (6) used in the lab?
A: The kit is used to prepare sequencing libraries for Hi-C, a technology that captures genome-wide chromatin interactions. Researchers process cultured cells or tissue samples following the straightforward manual protocol, producing libraries ready for next-generation sequencing.
Q: What sample types are compatible with this kit?
A: This kit is validated for use with both cultured cells and tissue samples, making it versatile for a broad range of epigenetic studies and chromatin interaction mapping projects.
Q: When will I receive results using the EpiTect Hi-C workflow?
A: Typically, the workflow can be completed within 1-2 days, allowing you to proceed to sequencing and downstream analysis soon after library preparation.
Q: Where should I store the kit for optimal performance?
A: To maintain reagent integrity, store the kit at -20C as indicated in the kit instructions. The kit is delivered on dry ice to ensure reagents remain stable during shipping.
Q: What are the primary benefits of using this Hi-C kit?
A: The EpiTect Hi-C Kit offers a highly streamlined workflow, high efficiency, and excellent reproducibility. Each lot is quality-control tested for consistent, high-accuracy results, supporting reliable epigenetic and chromatin studies.
Q: Is the kit compatible with all sequencing platforms?
A: Yes, the libraries prepared using this kit are compatible with major next-generation sequencing platforms, providing flexibility for different laboratory setups.