Qiagen QIAseq Human Exome Kit (96)

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Product Details:

Usage Type Laboratory
Features Uniform Coverage, High Sensitivity, Compatible With Low-Input DNA, Easy Workflow
Instruments Type NGS Library Prep Kit
Shelf Life 12 months (when stored as recommended)
Measurement Range Human Exonic Regions >19,396 genes
Function Targeted Capture of Human Exome DNA Regions
Accuracy High specificity target enrichment
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Qiagen QIAseq Human Exome Kit (96) Price And Quantity

Qiagen QIAseq Human Exome Kit (96) Product Specifications

Equipment Type Exome Enrichment Kit
Usage Type Laboratory
Features Uniform Coverage, High Sensitivity, Compatible With Low-Input DNA, Easy Workflow
Instruments Type NGS Library Prep Kit
Shelf Life 12 months (when stored as recommended)
Technology Next-Generation Sequencing (NGS), Hybrid Capture-Based Enrichment
Measurement Range Human Exonic Regions >19,396 genes
Material Biological Reagents, Enzymes, Buffers
Function Targeted Capture of Human Exome DNA Regions
Weight As per kit specification
Accuracy High specificity target enrichment
Condition New
Storage Instructions Store at -15C to -25C
Operating Type Manual and Automated Compatible
Wall Mounted No
Portable Yes
Use Targeted Exome Sequencing, Human Genomics Research
Real-Time Operation No
Dimension (L*W*H) Kit packaging as supplied by manufacturer

Product Description

Brand Qiagen

Product Type PCR Kit

Pack Size 96 Wells

CAT No 333939

Application Molecular Reagent

The 37 Mb QIAseq Human Exome panel delivers excellent coverage uniformity regardless of the target GC composition for sensitive detection of pathogenic variants, enabling time and cost efficiencies critical to scalability.

Efficient Targeted Exome Sequencing

The QIAseq Human Exome Kit allows for precise and streamlined capture of human exonic DNA regions, ensuring uniform coverage and high sensitivity even with low-input genomic samples. Leveraging next-generation sequencing (NGS) hybrid capture technology, researchers benefit from exceptional enrichment efficiency with over 98% target specificity. This enables robust mutation detection across more than 19,000 genes, supporting sophisticated analyses in genetic disease and cancer research.

Flexible Workflow and Throughput

This kit enables high-throughput processing of up to 96 samples per run, making it ideal for large-scale studies. Its protocol accommodates a wide range of DNA input amounts (101000 ng), providing flexibility for varied sample types or qualities. The protocol is compatible with manual or automated laboratory setups and can be completed in as little as 1.5 days, ensuring efficient lab operations without compromising performance or accuracy.

Comprehensive Content and Quality Control

The kit includes all necessary reagents for successful exome library preparation and enrichment: exome capture probes, buffers, magnetic beads, enzymes, and essential consumables. To maximize reliability, both positive and negative controls are incorporated for thorough process monitoring. A shelf life of 12 months and straightforward storage conditions (-15C to -25C) facilitate convenient laboratory management and long-term planning.

FAQs of Qiagen QIAseq Human Exome Kit (96):

Q: How does the QIAseq Human Exome Kit enhance genetic research workflows?

A: The kit streamlines library preparation and exome enrichment, offering high specificity capture of human exonic regions. Its reliable, easy-to-follow protocol boosts throughput, allows flexible DNA input, and accelerates workflows, making it ideal for studies in genetic disease and cancer genomics.

Q: What DNA input range is supported by the kit, and what types of samples are suitable?

A: The kit supports a broad DNA input range of 101000 ng, making it suitable for a variety of sample types, including challenging, low-yield or degraded samples from different sources, thus enhancing sample flexibility and utility in research.

Q: When should I use this exome enrichment kit over whole genome sequencing?

A: This kit is recommended when your research focuses on protein-coding regions of the genome. It is ideal for projects seeking to identify exonic variants associated with genetic diseases, cancer, or other traits, allowing for more cost-effective and targeted analysis compared to whole genome sequencing.

Q: Where can this kit be integrated within existing laboratory workflows?

A: The QIAseq Human Exome Kit is compatible with both manual and automated library prep systems that use Illumina sequencing platforms, making it easy to integrate into established NGS workflows in molecular biology or genomics laboratories.

Q: What is the process time for completing library prep and enrichment with this kit?

A: The combined library preparation and target enrichment workflow can be completed in approximately 1.5 days for all 96 samples, enabling rapid sample processing and timely results.

Q: How does the kit ensure accuracy and reproducibility across multiple samples?

A: It incorporates positive and negative controls within each run for robust quality control. The kits high enrichment specificity and uniform coverage ensure consistent and reliable data across all processed samples.

Q: What are the key benefits of using this kit in large cohort sequencing studies?

A: This kit supports high sample throughput, minimizes hands-on time, and guarantees high target specificity and sensitivity. Its flexible protocol and broad input compatibility enable streamlined processing of diverse samples, essential for large-cohort, high-impact genomics research.

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