Qiagen EZ1&2 DNA FFPE Kit (48)

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Product Details:

Measurement Range Typical DNA yield: 15 g from 2 sections (10 m each; depends on sample)
Shelf Life 12 months from date of manufacture
Function Automated DNA isolation from FFPE samples
Usage Type Laboratory research
Storage Instructions Store at room temperature (1525C), protect from light
Accuracy Yields DNA suitable for downstream molecular applications
Instruments Type EZ1 and EZ2 Advanced/Connect instruments
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Qiagen EZ1&2 DNA FFPE Kit (48) Price And Quantity

Qiagen EZ1&2 DNA FFPE Kit (48) Product Specifications

Wall Mounted No
Condition New
Storage Instructions Store at room temperature (1525C), protect from light
Accuracy Yields DNA suitable for downstream molecular applications
Instruments Type EZ1 and EZ2 Advanced/Connect instruments
Features Automation-ready, optimized for FFPE DNA isolation, high reproducibility, minimal cross-contamination, compatible with Qiagen EZ1 and EZ2 instruments
Color White/Transparent (reagents/columns)
Equipment Type DNA Extraction Kit
Dimension (L*W*H) Kit box dimensions: ~23 x 20 x 13 cm (approximate)
Measurement Range Typical DNA yield: 15 g from 2 sections (10 m each; depends on sample)
Portable Yes (kit format)
Shelf Life 12 months from date of manufacture
Technology Magnetic bead-based purification
Material Proprietary chemical reagents and spin columns
Real-Time Operation No
Operating Type Automatic (with EZ1/EZ2 instruments)
Usage Type Laboratory research
Function Automated DNA isolation from FFPE samples
Use Extraction of genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissues
Power Source Not Applicable (used with powered EZ1/EZ2 instruments)
Weight ~1.1 kg (kit box)
Noise Level Silent/Not Applicable

Product Description

Brand Qiagen

Product Type DNA Purification Kit

Pack Size 48T

CAT No 954404

Application Molecular Reagent

Purify large amounts of amplifiable DNA from hard-to-lyse formalin-fixed, paraffin-embedded (FFPE) tissue. The EZ1&2 DNA FFPE protocol uses double lysis to recover DNA effectively, while the optional uracil-N-glycosylase (UNG) step removes deaminated cytosine artifacts to limit the risk of nucleotide read errors.

Automated Efficiency for FFPE DNA Extraction

Experience streamlined DNA isolation from FFPE tissue with the Qiagen EZ1&2 DNA FFPE Kit. Each kit is optimized for use with Qiagen's EZ1 and EZ2 instruments, ensuring consistent, hassle-free workflow. With automated processes, you can process up to 14 samples per run, significantly reducing hands-on time and minimizing risk of errors compared to manual methods.

Optimized for Reliable Downstream Results

The kit employs magnetic bead-based technology to deliver high-quality DNA yields (typically 1-5 g) suitable for various molecular assays such as PCR, qPCR, and sequencing. Its proprietary reagents and spin columns ensure efficient removal of contaminants, providing DNA that meets the stringent requirements of research and analysis.

Simple Storage and Lasting Shelf Life

This DNA extraction kit is supplied in a compact, portable box, weighing approximately 1.1 kg. All reagents are stable at room temperature (15-25C), protected from light, and provide a 12-month shelf life from the manufacturing date. Its robust design allows for straightforward integration into most laboratory workflows.

FAQ's of Qiagen EZ1&2 DNA FFPE Kit (48):

Q: How do I use the Qiagen EZ1&2 DNA FFPE Kit for DNA extraction from FFPE tissues?

A: To use the kit, first prepare FFPE tissue sections (1-10 mg), place them in compatible tubes, and load them onto the EZ1 or EZ2 Advanced/Connect instrument. The automated system will carry out lysis, binding, washing, and elution processes, providing purified DNA ready for downstream analysis.

Q: What types of samples can be processed with this kit?

A: The kit is specifically designed for extracting genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections, making it ideal for archived clinical and research specimens.

Q: When should I choose this kit for my laboratory workflow?

A: Choose this kit when you need reliable, automated DNA extraction from FFPE samples for molecular assays such as PCR, qPCR, or sequencing, and want to reduce manual processing and contamination risk.

Q: Where can the Qiagen EZ1&2 DNA FFPE Kit be used?

A: This kit is intended for laboratory research use and is suitable for use where Qiagen EZ1 or EZ2 Advanced/Connect instruments are available, including academic, diagnostic, and pharmaceutical settings.

Q: What is the maximum throughput per run, and how many extractions are supported per kit?

A: On the EZ1 instrument, up to 6 samples can be processed per run, and on the EZ2 instrument, up to 14. Each kit supports 48 DNA preparations, with all necessary reagents and columns included.

Q: What are the main benefits of using this kit compared to manual methods?

A: The main benefits include automation-ready protocols, high reproducibility, reduced cross-contamination, and reliable yields of high-quality DNA suitable for sensitive molecular applications-saving both time and labor in the laboratory.

Q: How should the kit be stored to maintain reagent quality?

A: Store the kit at room temperature (15-25C) and protect the contents from light. Under these conditions, the reagents remain stable for up to 12 months from the date of manufacture.

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