Product Description
Brand Waters Corp
Product Type Kit
Pack Size 1 Nos
CAT No 176004151
Application Glycan
The GlycoWorks RapiFluor-MS N-Glycan Script Starter Kit Automation has been designed for use with automated platforms enabling high-throughput glycoprotein sample preparation for analysis of N-glycans with minimal user intervention. This protocol is validated using monoclonal antibodies and has also been tested to perform for a wide range of other N-linked glycoproteins.
Accelerate N-Glycan AnalysisDesigned to streamline glycan sample preparation, the RapiFluor-MS N-Glycan Script Starter Kit enables rapid processing and robust automation. Up to 96 samples can be handled efficiently with automation accessories, making it ideal for research and QC environments demanding high-throughput data. Each script run can process 24 samples, ensuring flexibility across workflows.
Exceptional Detection SensitivityThe kit supports both fluorescent and mass spectrometric detection, with sensitivity reaching femtomole levels. Featuring optimized protocols and RapiFluor-MS reagent, N-glycans are labeled for enhanced performance on Waters ACQUITY UPLC/LC-MS systems as well as other compatible platforms, minimizing sample loss and maximizing data accuracy.
Reliable and Safe Glycan PreparationConstructed for automated processes, all reagents are non-toxic and non-volatile, supported by comprehensive SDS documentation. The protocol files are tailored for major liquid handlers, simplifying setup for busy labs. CE-marked and RoHS compliant, the kit supports secure and regulatory-compliant glycoprotein analysis in various laboratory settings.
FAQ's of Waters Corp GlycoWorks RapiFluor-MS N-Glycan Script Starter Kit-Automation:
Q: How does the GlycoWorks RapiFluor-MS N-Glycan Script Starter Kit benefit automated glycoprotein analysis?
A: The kit dramatically streamlines glycan sample processing by supporting automation on liquid handling platforms. With rapid labeling times as short as 30 minutes and ability to process up to 96 samples (automation dependent), it enables high-throughput, reproducible analysis with minimal manual intervention, making it ideal for research and QC labs.
Q: What types of samples can be processed using this kit?
A: The kit is designed for a wide range of sample types, including monoclonal antibodies, serum proteins, and recombinant glycoproteins. Its broad compatibility and optimized reagents ensure consistent glycan release and labeling from various glycoprotein sources.
Q: What detection sensitivity does this kit provide for glycan analysis?
A: With RapiFluor-MS technology, the kit achieves detection sensitivity up to femtomole levels for both fluorescence and mass spectrometry. This facilitates accurate identification and quantitation even in low-abundance glycan samples.
Q: When should kit components be stored and for how long are they usable?
A: Kit components should be stored at 2-8C for optimal stability. The kit remains effective for 12 months from the date of manufacture when stored under recommended conditions, ensuring ongoing reliability for periodic testing.
Q: Where is this kit most appropriately used in laboratory workflows?
A: It is best suited for high-throughput research and biopharmaceutical QC laboratories, where automated glycoprotein sample preparation and N-glycan analysis are routine. The kit's automation-grade format integrates smoothly into major liquid handling systems.
Q: What is the process for preparing samples using the kit's automation script?
A: The kit includes a detailed protocol file (script) optimized for major automation platforms. Users simply follow the step-by-step instructions to release, label, and purify N-glycans, utilizing pre-filled plates and reagents. One script run handles 24 samples; accessories are available to scale throughput as needed.
Q: What are the main advantages of using RapiFluor-MS labeling in glycan analysis?
A: RapiFluor-MS enables rapid and robust fluorescent labeling, providing enhanced MS signal response and high recovery rates (typically over 90%). This improves sensitivity, accuracy, and reproducibility in glycan profiling compared to conventional methods.