Qiagen REPLI-g Screening Kit (200)

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Product Details:

Instruments Type Molecular Biology Kit
Measurement Range Nanogram to microgram range of DNA input
Usage Type Laboratory/Research
Features High fidelity amplification, uniform genome coverage, rapid workflow
Function Amplifies genomic DNA for downstream genomic analysis
Storage Instructions -20C; protect from light
Shelf Life 12 months
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Qiagen REPLI-g Screening Kit (200) Price And Quantity

Qiagen REPLI-g Screening Kit (200) Product Specifications

Shelf Life 12 months
Equipment Type DNA Amplification Kit
Features High fidelity amplification, uniform genome coverage, rapid workflow
Storage Instructions -20C; protect from light
Function Amplifies genomic DNA for downstream genomic analysis
Measurement Range Nanogram to microgram range of DNA input
Real-Time Operation No
Usage Type Laboratory/Research
Condition New
Instruments Type Molecular Biology Kit
Operating Type Manual
Use Whole Genome Amplification for screening applications
Technology Multiple Displacement Amplification (MDA)
Portable Yes
Wall Mounted No
Color White
Accuracy High (suitable for sensitive molecular analysis)
Material Reagents and Buffer Solutions

Product Description

Brand Qiagen

Product Type PCR Kit

Pack Size 200T

CAT No 150126

Application Molecular Reagent

Limitations on the number and type of genomic analyses that can be performed due to insufficient quantities of genomic DNA can be overcome by global amplification of all DNA within a sample (whole genome amplification). REPLI?g Kits provide DNA polymerase, buffers and reagents for highly uniform whole genome amplification with minimal sequence bias and can be used with various starting materials, including genomic DNA, fresh or dried blood, buccal swabs, fresh or frozen tissue and cells. QIAGEN REPLI-g Kits are available in several different sizes and configurations which allows researchers to build the best workflow for their application and lab space. Typical REPLI-g yields range from approx. 7 g to 40 g of amplified DNA, depending on which kit and workflow is used.

Reliable and Efficient Screening

The REPLI-g Screening Kit provides dependable, high yield DNA amplification suitable for applications requiring accurate genomic representation. Its robust design ensures uniform genome coverage and high fidelity, making it a powerful tool for sensitive molecular analyses in research laboratories.

Adaptable for Various Workflows

With its compatibility for automated and manual processing, this kit streamlines workflows in a range of laboratory environments. Its straightforward protocol, provided within the package, facilitates ease of use and reliable results in screening projects of varying scales.

Broad Sample Compatibility

The kit is designed to amplify DNA from diverse sources, including human, animal, and plant genomes. This broad compatibility allows researchers in genomics, diagnostics, and agricultural studies optimal flexibility with a single, versatile DNA amplification solution.

FAQ's of Qiagen REPLI-g Screening Kit (200):

Q: How do I use the Qiagen REPLI-g Screening Kit for whole genome amplification?

A: To use the REPLI-g Screening Kit, prepare input DNA (1-10 ng), mix with the provided reagents and buffers, and incubate the reaction for approximately 8 hours according to the included protocol. The amplified product can then be used for downstream genomic analyses.

Q: What are the primary benefits of using this kit in molecular biology research?

A: The REPLI-g Screening Kit offers rapid amplification, high yield (up to 40 g per reaction), and excellent genome coverage with high fidelity. These qualities support sensitive and reliable results, making it ideal for screening and downstream DNA applications.

Q: When should I use this kit in my workflow?

A: This kit is best deployed whenever high-quality whole genome amplification is needed for screening a variety of DNA sources, such as when starting with limited material or preparing for large-scale genomic studies.

Q: Where can the REPLI-g Screening Kit be used?

A: The kit is suitable for laboratory settings in research, diagnostics, agriculture, and academia. Its design is not specific to any one environment and is widely used in settings where molecular DNA analysis is required.

Q: What DNA samples are compatible with the kit?

A: The REPLI-g Screening Kit is optimized for amplification of human, animal, and plant genomic DNA, allowing broad species compatibility for numerous research applications.

Q: What is the shelf life and recommended storage conditions for the kit?

A: The kit has a 12-month shelf life and should be stored at -20C, protected from light, to maintain the integrity of its reagents and ensure reliable performance.

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