Qiagen EpiTect 96 Bisulfite Kit (2)

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Product Details:

Instruments Type Laboratory reagent kit
Function DNA bisulfite conversion for methylation profiling
Shelf Life 12 months (from production date under recommended conditions)
Storage Instructions Store at 28C (reagents)
Features High conversion efficiency, convenient 96-well format, optimized protocols, complete reagents included, minimized DNA degradation
Accuracy >99% conversion of unmethylated cytosines
Usage Type Laboratory, Research use only
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Qiagen EpiTect 96 Bisulfite Kit (2) Price And Quantity

Qiagen EpiTect 96 Bisulfite Kit (2) Product Specifications

Shelf Life 12 months (from production date under recommended conditions)
Portable Yes
Condition New
Color Standard (as per chemical/reagent standards)
Use DNA methylation analysis, bisulfite conversion for downstream PCR, sequencing, and methylation-specific analysis
Technology Sodium bisulfite conversion of unmethylated cytosines in DNA
Weight Approx. 3.5 kg (kit, including all components)
Material Plastic consumables, chemicals, reagents
Storage Instructions Store at 28C (reagents)
Real-Time Operation No
Equipment Type Bisulfite Conversion Kit (High-Throughput, 96-well Format)
Accuracy >99% conversion of unmethylated cytosines
Features High conversion efficiency, convenient 96-well format, optimized protocols, complete reagents included, minimized DNA degradation
Operating Type Manual or Automated (compatible with liquid handlers)
Measurement Range 502000 ng input DNA per reaction
Dimension (L*W*H) Kit box dimensions vary (consult datasheet)
Instruments Type Laboratory reagent kit
Function DNA bisulfite conversion for methylation profiling
Usage Type Laboratory, Research use only
Wall Mounted No

Product Description

Brand Qiagen

Product Type PCR Kits

Pack Size 96 Wells

CAT No 59110

Application Molecular Reagent

piTect Bisulfite Kits enable complete conversion of unmethylated cytosines to uracils and subsequent purification in 6 hours. The EpiTect 96 Bisulfite procedure takes less than 7 hours. The highly sensitive method utilizes innovative protection against DNA degradation that guarantees sensitive results, even from 1 ng DNA, and ensures high conversion rates of over 99%. Due to its unique DNA Protect technology, EpiTect Bisulfite converted DNA is highly suitable for whole bisulfitome amplification using EpiTect Whole Bisulfitome Kits. This enables reliable amplification of DNA in cases where bisulfite converted DNA is limited. EpiTect Bisulfite Kits include a spin-column or 96-well format procedure, and can be processed using a centrifuge or vacuum manifold. In addition, the EpiTect Bisulfite Kit can be fully automated on theQIAcube Connect.

Efficient High-Throughput Bisulfite Conversion

Handle up to 96 samples simultaneously with the EpiTect 96 Bisulfite Kit's convenient 96-well plate format. This allows for increased sample throughput, consistency in DNA processing, and compatibility with both manual and automated platforms, making it ideal for modern, high-demand research laboratories.

Optimized for Accurate Methylation Analysis

Achieve reliable methylation profiling thanks to the kit's high bisulfite conversion efficiency (>99%) and minimized DNA degradation. The kit includes all necessary reagents and follows refined protocols, ensuring reproducible results for downstream applications such as PCR, NGS, and Sanger sequencing.

Complete Solution for DNA Methylation Studies

Supplied with all consumables and chemicals-buffers, bisulfite solution, DNA protect and desulfonation buffers, plates, and sealing films-this kit covers every step of bisulfite conversion from DNA extraction to sample prep for further analysis. It suits research laboratories focused on epigenetic studies and biomarker discovery.

FAQ's of Qiagen EpiTect 96 Bisulfite Kit (2):

Q: How does the Qiagen EpiTect 96 Bisulfite Kit facilitate DNA methylation analysis?

A: The kit enables sodium bisulfite conversion of unmethylated cytosines in genomic DNA, preparing samples for downstream methylation analysis by methods such as PCR, sequencing, and NGS.

Q: What is the minimum and maximum amount of DNA input required per reaction?

A: Each reaction requires between 50 ng and 2000 ng of input genomic DNA, providing a broad range for sample types and experimental needs.

Q: What types of applications is this kit compatible with?

A: The kit is suitable for PCR, methylation-specific PCR (MSP), pyrosequencing, Sanger sequencing, and next-generation sequencing (NGS) after bisulfite conversion.

Q: How long does the complete bisulfite conversion process take?

A: The total processing time, including all incubation steps, is approximately 6 hours for up to 96 samples.

Q: Can this kit be used with automated liquid handling systems?

A: Yes, the kit is compatible with robotic liquid handling platforms as well as manual operation, enhancing its flexibility for high-throughput laboratories.

Q: Where should the kit reagents be stored to maintain stability?

A: All reagents should be stored at 2-8C as indicated to ensure optimal shelf life and performance, which is 12 months from production.

Q: What are the key benefits of using this bisulfite conversion kit?

A: The kit offers high conversion efficiency, reduced DNA degradation, a complete set of necessary reagents, streamlined protocols, suitability for multiple downstream analyses, and compatibility with high-throughput and automated workflows.

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