Qiagen HotStarTaq DNA Polymerase (25000)

Qiagen HotStarTaq DNA Polymerase (25000)

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Product Details:

Instruments Type Molecular Biology Reagent
Shelf Life Typically 24 months from date of manufacture
Function Thermostable DNA polymerase for PCR
Measurement Range Template DNA from 10 pg to 1 g
Usage Type Laboratory reagent
Storage Instructions Store at -20C
Features HotStart activation, high specificity, reduced primer-dimer formation
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Qiagen HotStarTaq DNA Polymerase (25000) Price And Quantity

Qiagen HotStarTaq DNA Polymerase (25000) Product Specifications

Instruments Type Molecular Biology Reagent
Material Enzyme, supplied in storage buffer
Color Colorless (enzyme solution)
Use PCR amplification of DNA
Shelf Life Typically 24 months from date of manufacture
Function Thermostable DNA polymerase for PCR
Condition New
Portable Yes (reagent)
Technology Hot-start PCR technology
Wall Mounted No
Measurement Range Template DNA from 10 pg to 1 g
Real-Time Operation No
Equipment Type DNA Polymerase
Usage Type Laboratory reagent
Storage Instructions Store at -20C
Operating Type Manual (requires thermal cycler)
Features HotStart activation, high specificity, reduced primer-dimer formation
Accuracy High specificity and yield in PCR

Catalog Number 203205
Buffer Included 10x PCR buffer (with MgCl2) provided
Supplier Qiagen
Application Standard and complex PCR, genotyping, cloning, sequencing templates
Packaging Size 25,000 units
Stabilizer Supplied in stabilizing storage buffer
Compatibility Compatible with most PCR thermal cyclers
Activation Method 15 min initial heating at 95C required
Concentration 5 U/l in storage buffer

Product Description

Brand Qiagen

Product Type PCR Kits

Pack Size 2500 ul

CAT No 203209

Application Molecular Reagent

HotStarTaq DNA Polymerase uses a chemically mediated hot start that, unlike, antibody-mediated systems, leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR.HotStarTaq DNA Polymerase is supplied with the unique QIAGEN PCR Buffer, which minimizes nonspecific amplification products, primer dimers, and background. Q-Solution, a novel additive that enables efficient amplification of "difficult" (e.g., GC rich) templates, is also provided.

Hot-Start Activation for Enhanced PCR Specificity

Qiagen HotStarTaq DNA Polymerase harnesses hot-start PCR technology, activated by a simple 15-minute incubation at 95C. This process blocks enzyme activity at low temperatures, drastically reducing non-specific amplification and primer-dimer formation. The result is a streamlined workflow for both routine and challenging PCR applications requiring high precision.

Reliable Performance for Diverse Applications

Designed to handle everything from standard to complex PCR, genotyping, cloning, and DNA sequencing, this enzyme offers high specificity and robust amplification from template DNA ranging from 10 pg to 1 g. Its compatibility with most thermal cyclers and inclusion of a 10x PCR buffer make it a versatile choice for laboratories with diverse needs.

Convenient, Large-Scale Packaging for Laboratories

Packaged with 25,000 units at 5 U/l in a stabilizing storage buffer, Qiagen HotStarTaq DNA Polymerase ensures reliability and cost-effectiveness for laboratories with high throughput demands. The enzyme retains stability for up to 24 months when stored at -20C, ensuring consistent results throughout its shelf life.

FAQ's of Qiagen HotStarTaq DNA Polymerase (25000):

Q: How do I properly activate Qiagen HotStarTaq DNA Polymerase before my PCR run?

A: HotStarTaq DNA Polymerase requires a 15-minute initial heating step at 95C to activate the enzyme. This step is essential for the hot-start technology to provide high specificity and reduce non-specific amplification during PCR.

Q: What types of applications is this polymerase suitable for?

A: This enzyme is optimized for standard and complex PCR, genotyping, cloning, and sequencing templates, making it a versatile reagent for molecular biology laboratories.

Q: When should I use Qiagen HotStarTaq DNA Polymerase over a regular Taq enzyme?

A: Use this hot-start polymerase when high specificity is crucial, such as in multiplex PCR, complex templates, or when primer-dimer formation is a concern. The hot-start mechanism improves accuracy and yields in difficult PCR reactions.

Q: Where can Qiagen HotStarTaq DNA Polymerase be used?

A: This reagent is intended for use in laboratories with standard PCR equipment. It is compatible with most commercially available thermal cyclers, offering flexibility for different experimental setups.

Q: What processes are simplified by the inclusion of 10x PCR buffer with MgCl2?

A: The supplied 10x PCR buffer streamlines PCR setup by providing optimized conditions for DNA amplification, eliminating the need to prepare separate buffers and ensuring reliable performance.

Q: How should I store HotStarTaq DNA Polymerase for optimal shelf life?

A: To maintain enzyme activity and stability, store the HotStarTaq DNA Polymerase at -20C. The enzyme is stable for up to 24 months from the date of manufacture under these conditions.

Q: What are the main benefits of using this polymerase for PCR amplification?

A: Key benefits include high specificity due to hot-start technology, robust yields across a wide range of template concentrations, reduced primer-dimer formation, and straightforward integration into various PCR workflows.

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