Qiagen REPLI-g Cell WGA & WTA Kit (48)

Get a Price/Quote

Product Details:

Measurement Range From single cell up to 1,000 cells per reaction
Storage Instructions Store at -20C
Usage Type Laboratory use only
Features High amplification yield, accurate genome representation, suitable for single cells, parallel WGA and WTA workflows
Instruments Type Molecular biology reagent kit
Accuracy High-fidelity amplification (isothermal polymerase)
Function Simultaneous whole genome amplification (WGA) and whole transcriptome amplification (WTA)
Click to View more
Share Your Product:

Request to Call Back Send Inquiry

Qiagen REPLI-g Cell WGA & WTA Kit (48) Price And Quantity

Qiagen REPLI-g Cell WGA & WTA Kit (48) Product Specifications

Storage Instructions Store at -20C
Portable Yes (kit format)
Color White/Transparent (reagents in kit)
Measurement Range From single cell up to 1,000 cells per reaction
Technology Multiple Displacement Amplification (MDA) and Reverse Transcription (RT)
Material Reagents and Enzymes
Use Amplification of genomic DNA and cDNA synthesis from single or few cells
Wall Mounted No
Condition New
Shelf Life Refer to product packaging; usually 12 months from manufacturing
Real-Time Operation No
Equipment Type Whole Genome Amplification & Transcription Kit
Operating Type Manual
Features High amplification yield, accurate genome representation, suitable for single cells, parallel WGA and WTA workflows
Usage Type Laboratory use only
Function Simultaneous whole genome amplification (WGA) and whole transcriptome amplification (WTA)
Accuracy High-fidelity amplification (isothermal polymerase)
Instruments Type Molecular biology reagent kit

Product Description

Brand Qiagen

Product Type WTA Kit

Pack Size 48 Wells

CAT No 150054

Application Molecular Reagent

The REPLI-g Cell WGA & WTA Kit uniquely enables uniform whole genome amplification (WGA) and whole transcriptome amplification (WTA) in parallel reactions, allowing direct correlation of the genome with the transcriptome from very small samples (251000 cells). Dedicated buffers and reagents undergo a unique, controlled decontamination procedure to block amplification of contaminating nucleic acids by the REPLI-g method. The innovative lysis buffer effectively stabilizes cellular nucleic acids, ensuring accurate representation of both genomic DNA and RNA levels. Unbiased amplification of genomic DNA and total RNA or mRNA (poly A+) is achieved using innovative Multiple Displacement Amplification (MDA) technology and the novel REPLI-g SensiPhi DNA Polymerase. In contrast to PCR-based amplification technologies, high fidelity rates are increased up to 1000-fold, avoiding costly false positive or negative results.

Comprehensive Single-Cell Analysis

The REPLI-g Cell WGA & WTA Kit streamlines amplification of both genomic DNA and cDNA from single cells or small cell populations. Its robust reagents deliver unmatched sensitivity and high yields, making it an indispensable tool for researchers working in single-cell genomics, transcriptomics, and downstream NGS library prep.

Efficient and Accurate Amplification

Utilizing Multiple Displacement Amplification and advanced RT chemistry, this kit achieves strong, unbiased genome and transcriptome representation. High-fidelity polymerase minimizes sequence errors, supporting the generation of reliable data from minimal input. Reaction times are optimized: WGA completes in ~8 hours, while WTA is finished in ~2.5 hours.

Flexible Application and User-Friendly Design

This kit is compatible with a broad range of eukaryotic samples, including human and animal cells. It comes with all necessary reagents and detailed protocols. The kit is manually operated and portable, suitable for various laboratory setups. Its dry ice shipping ensures enzyme integrity, and components remain stable at -20C.

FAQ's of Qiagen REPLI-g Cell WGA & WTA Kit (48):

Q: How does the REPLI-g Cell WGA & WTA Kit amplify DNA and cDNA from single cells?

A: The kit uses Multiple Displacement Amplification (MDA) for whole genome amplification and Reverse Transcription (RT) for whole transcriptome amplification, enabling simultaneous high-yield and accurate DNA and cDNA generation from single or few cells.

Q: What types of samples are compatible with this kit?

A: This kit is designed for single cells or small numbers of sorted cells from human, animal, or other eukaryotic species, allowing for flexibility in research applications.

Q: When should I choose this kit for my experiments?

A: Select the REPLI-g Cell WGA & WTA Kit when you require high-fidelity amplification from minute cellular material-for example, in single-cell genomics, gene expression profiling, or next-generation sequencing library preparation.

Q: Where should the kit be stored and are there specific storage requirements?

A: Store the kit at -20C to maintain reagent activity and shelf life. Refer to the product packaging for detailed expiry information, typically 12 months from the manufacturing date.

Q: What is the process for using the kit, and what is included?

A: The process involves cell lysis, reverse transcription (for WTA), and isothermal amplification. The kit contains Cell Lysis Buffer, REPLI-g sc DNA Polymerase, Mastermix, RT Mix, Nuclease-Free Water, and a protocol booklet to guide your work.

Q: Can the kit support downstream next-generation sequencing workflows?

A: Yes, the high-yield and unbiased amplification products generated by the kit are suitable for various downstream applications, including NGS library preparation for both DNA and RNA analyses.

Q: What are the primary benefits of using this kit in a laboratory setting?

A: Its key advantages include high amplification yields, excellent genome and transcriptome representation, parallel WGA and WTA capabilities, compatibility with diverse eukaryotic samples, and manual, user-friendly operation.

Tell us about your requirement

Price:

Quantity

Select Unit

50
100
200
250
500
1000+

Additional detail

Mobile number

Other Products in 'Medical Reagents' category

Snibe MAGLUMI Vitamin B12 (CLIA) Storage Instructions : Store at 28C Function : Vitamin B12 Quantification Features : High Sensitivity, Specificity, Rapid Results, Traceable Calibration, Reproducible Usage Type : Invitro Diagnostic Use Only Use : Quantitative Detection of Vitamin B12 in Human Serum/Plasma Technology : Chemiluminescent Immunoassay (CLIA) Portable : No Color : White (Reagent Bottles) Instruments Type : Reagent Kit for MAGLUMI CLIA Series Wall Mounted : No Condition : New Material : Other, Diagnostic Reagent, Solution and Components Operating Type : Other, Fully Automatic Accuracy : 95% (as per manufacturer data) Measurement Range : 502000 pg/mL Shelf Life : 12 Months Weight : Not Applicable (Reagent Kit) Noise Level : Low/Negligible Equipment Type : Vitamin B12 Analyzer Reagent Kit Dimension (L*W*H) : Not Applicable (Reagent Bottles) Real-Time Operation : Yes Frequency : Depends on Instrument Protocol Power Source : Other, External Automated Analyzer Required

Send Inquiry

Mindray Calcium Reagent Kits Storage Instructions : Store at 28C, avoid direct sunlight Function : Quantitative estimation of calcium in serum Features : Ready to use reagents, high accuracy, compatible with Mindray analyzers Usage Type : Laboratory, Clinical Diagnosis Use : Serum Calcium Determination for Clinical Laboratory Technology : Colorimetric Assay Principle Portable : Yes, as kit Color : Clear/Colorless reagent Instruments Type : Compatible with Mindray Chemistry Analyzers Wall Mounted : No Condition : New Material : Other, Plastic/Glass Vials (Reagent Grade) Operating Type : Other, Automatic (for use with Mindray analyzers) Accuracy : CV 2% Measurement Range : 0.3 20 mg/dL Shelf Life : 24 months from manufacturing date Weight : Approx. 200g per kit Noise Level : 58 dB Equipment Type : Calcium Reagent Kit Dimension (L*W*H) : Standard kit packaging (refer to manual) Real-Time Operation : Yes Frequency : As per analyzer protocol Power Source : Other, Not Applicable (analyzer powered)

Send Inquiry

Thermo Fisher Scientific Invitrogen PureLink PCR Micro Kit Storage Instructions : Store at room temperature (1530C) Function : Extraction and purification of DNA from PCR reactions Features : Efficient DNA recovery, rapid protocol, compatible with microscale samples Usage Type : Laboratory/Research use (for in vitro studies only) Use : For purification of PCR products (DNA) from enzymes, primers, and dNTPs Technology : Silica membrane spin column technology Portable : Yes Color : White (columns) / Multicolor labeling for kit components Instruments Type : Molecular Biology Kit Wall Mounted : No Condition : New Material : Other, Highquality reagent grade chemicals, silicabased membrane Operating Type : Manual Accuracy : Up to 95% recovery of DNA fragments Measurement Range : Purifies fragments from 100 bp to 12 kb Shelf Life : 1 year from date of manufacture Weight : Not Applicable (Kit format) Equipment Type : PCR Purification Kit Dimension (L*W*H) : Not Applicable (Kit format) Real-Time Operation : No

Send Inquiry

FUJIFILM FUJI DRI-CHEM SLIDE CHE-P Storage Instructions : Store in a cool dry place Function : Diagnostic Testing Features : Accurate Easy to Use Usage Type : OneTime Use Use : Clinical Diagnostics Technology : Dry Chemistry Portable : Yes Color : White/Green Instruments Type : Diagnostic Consumable Wall Mounted : No Condition : New Material : Chemical/Plastic, Other Operating Type : Other, Automated Accuracy : High Measurement Range : Depends on Test Type Shelf Life : Standard Shelf Life (Refer to packaging) Weight : Lightweight Noise Level : Silent

Send Inquiry

SEND INQUIRY CALL ME FREE