Qiagen REPLI-g WTA Single Cell Kit (96)

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Product Details:

Usage Type Laboratory/Research
Storage Instructions Store components at -20C; some buffers at 28C as per datasheet
Shelf Life 12 months
Function Amplifies and converts minute amounts of RNA from single cells to cDNA for downstream applications
Measurement Range Single cell RNA (~10 pg RNA per cell)
Instruments Type Molecular biology reagent kit
Features High sensitivity and yield, single-cell total RNA amplification, isothermal reaction, streamlined workflow
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Qiagen REPLI-g WTA Single Cell Kit (96) Price And Quantity

Qiagen REPLI-g WTA Single Cell Kit (96) Product Specifications

Technology Multiple displacement amplification (MDA) of cDNA
Use Amplification of total RNA from single cells for transcriptome analysis
Accuracy High reproducibility for single-cell cDNA synthesis
Instruments Type Molecular biology reagent kit
Equipment Type Single Cell Whole Transcriptome Amplification Kit
Features High sensitivity and yield, single-cell total RNA amplification, isothermal reaction, streamlined workflow
Storage Instructions Store components at -20C; some buffers at 28C as per datasheet
Color White/Blue (as per Qiagen standard kit packaging)
Shelf Life 12 months
Portable Yes
Material Biological reagents (specially formulated enzymatic components, buffers and primers)
Dimension (L*W*H) Kit box dimensions approx. 27cm x 20cm x 13cm
Condition New
Function Amplifies and converts minute amounts of RNA from single cells to cDNA for downstream applications
Measurement Range Single cell RNA (~10 pg RNA per cell)
Wall Mounted No
Operating Type Manual
Real-Time Operation No
Weight Approx. 1.2 kg
Usage Type Laboratory/Research

Product Description

Brand Qiagen

Product Type PCR Kit

Pack Size 96 Wells

CAT No 150065

Application Molecular Reagent

The REPLI-g WTA Single Cell Kit enables reliable investigation of effects on transcription regulation at the single-cell transcriptome level and allows uniform amplification of all transcripts from just single cells (11000 cells). Dedicated buffers and reagents undergo a unique, controlled decontamination procedure to block amplification of contaminating nucleic acids by the REPLI-g method. The innovative lysis buffer effectively stabilizes cellular RNA, ensuring the resulting RNA accurately reflects the in vivo gene expression profile. All enzymatic steps have been developed to enable efficient processing of RNA for accurate amplification of cDNA, which is achieved with negligible sequence bias using innovative Multiple Displacement Amplification (MDA) technology.

Reliable Amplification from Single Cells

This kit is engineered to amplify and convert minuscule RNA quantities directly from single cells into cDNA, making it possible to analyze gene expression at the individual cell level. Its advanced enzymatic formulation ensures high sensitivity and consistent results, crucial for projects in transcriptomics and single-cell studies.

Flexible Application Range

From fresh to frozen samples, including both microdissected and FACS-sorted cells, the kit's flexibility supports broad research purposes. Its compatibility with major downstream analysis systems, including next-generation sequencing (NGS) and quantitative PCR, streamlines your transition from sample preparation to data acquisition and interpretation.

Kit Convenience and Quality Assurance

All components-SensiPhi DNA Polymerase, buffers, primers, nuclease-free water, and quality control RNA-are individually packaged to ensure reagent integrity and workflow clarity. The kit also features a detailed protocol, barcoded packaging for traceability, and a reliable shelf life when stored according to instructions.

FAQ's of Qiagen REPLI-g WTA Single Cell Kit (96):

Q: How does the Qiagen REPLI-g WTA Single Cell Kit amplify RNA from single cells?

A: The kit utilizes multiple displacement amplification (MDA) technology to synthesize and amplify cDNA from total RNA in single cells. SensiPhi DNA Polymerase and specially formulated reagents enable high-fidelity, isothermal amplification, yielding sufficient cDNA for downstream analysis.

Q: What types of samples can be processed with this kit?

A: You can use single cells from a variety of sources-fresh, frozen, microdissected, or sorted (such as by FACS). This versatility allows the kit to be integrated into many workflows addressing different research needs.

Q: When should I use this kit in my research workflow?

A: The kit is ideal when you require amplification of transcriptomes from extremely small samples-such as where single-cell RNA quantity is limiting. It is typically used prior to applications like RNA-seq, gene expression profiling, or single-cell analysis.

Q: Where is the kit typically used?

A: This kit is intended for laboratory and research use. It is a key tool in molecular biology labs working on single-cell genomics, transcriptomics, or applications requiring sensitive cDNA amplification from limited starting material.

Q: What is the process duration and throughput for a standard run?

A: A single reaction typically takes about 4-6 hours. Each kit contains enough reagents to perform 96 reactions, supporting high-throughput studies or parallel sample analysis.

Q: How are the kit components stored and what is their shelf life?

A: Reagents should be stored according to the datasheet: most at -20C, with some buffers between 2-8C. When stored properly, the kit maintains performance for up to 12 months from the date of manufacture.

Q: What are the main benefits of using this kit for single-cell analysis?

A: It provides exceptional sensitivity and yield, high reproducibility for cDNA synthesis from minimal RNA, and broad compatibility with NGS and qPCR platforms. The streamlined workflow and robust quality controls enhance reliability and ease-of-use.

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