Qiagen Cell and Gene Therapy Viral Vector Lysis Kit (100 rxn)

Qiagen Cell and Gene Therapy Viral Vector Lysis Kit (100 rxn)

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Product Details:

Usage Type Research Use Only
Accuracy Optimized for maximum lysis and nucleic acid yield
Instruments Type Sample Preparation Kit
Shelf Life 12 months from date of manufacture
Features High efficiency lysis, compatible with various viral vectors
Storage Instructions Store at 28C
Function Lysis of viral vectors for downstream nucleic acid extraction
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Qiagen Cell and Gene Therapy Viral Vector Lysis Kit (100 rxn) Price And Quantity

Qiagen Cell and Gene Therapy Viral Vector Lysis Kit (100 rxn) Product Specifications

Use Cell and gene therapy, viral vector processing for nucleic acid purification
Dimension (L*W*H) Kit box dimensions: 20 cm 18 cm 8 cm (approx.)
Instruments Type Sample Preparation Kit
Shelf Life 12 months from date of manufacture
Condition New
Usage Type Research Use Only
Wall Mounted No
Equipment Type Viral Vector Lysis Kit
Accuracy Optimized for maximum lysis and nucleic acid yield
Storage Instructions Store at 28C
Operating Type Manual
Weight Approx. 0.85 kg
Real-Time Operation No
Function Lysis of viral vectors for downstream nucleic acid extraction
Material High-purity chemical reagents
Technology Optimized buffer chemistry for viral vector lysis
Portable Yes
Features High efficiency lysis, compatible with various viral vectors
Color White/Transparent

Product Description

Brand Qiagen

Product Type Vector Lysis Kit

Pack Size 100T

CAT No 250272

Application Molecular Reagent

The CGT Viral Vector Lysis Kit is provided with reagents for 100 or 1000 reactions in two boxes for AAV vectors. The kit is suitable for the lysis of AAV2, AAV5, AAV6, AAV8 and AAV9. The lysates are optimized forQIAcuity Cell and Gene Therapy dPCR Assaysin combination with theQIAcuity Probe PCR Kit. These assays enable singleplex as well as multiplex cell and gene therapy applications, including viral titer and vector copy number measurements. The kit does not include restriction enzymes (for e.g., Hpall) that are stated in the protocol.

Optimized for Versatile Viral Vectors

This lysis kit is designed to efficiently disrupt a broad spectrum of viral vectors, such as adeno-associated virus (AAV), lentivirus, adenovirus, and more. Its advanced buffer chemistry maximizes nucleic acid yield while preserving sample quality, making it invaluable for cell and gene therapy environments.

Streamlined Workflows and Reliable Results

Supplied with straightforward protocols and pre-formulated buffers, the kit integrates effortlessly into manual and automated sample preparation pipelines. It is optimized for high lysis efficiency, enabling consistent, high-purity nucleic acid recovery that accelerates downstream applications in research.

Robust and Safe for Research Use Only

Composed of high-purity, single-use reagents, the kit offers robust performance and minimizes cross-contamination. Each kit comes with a unique lot number and clear storage instructions, ensuring reliable results with every use. Not intended for diagnostic procedures; suitable strictly for research purposes.

FAQ's of Qiagen Cell and Gene Therapy Viral Vector Lysis Kit (100 rxn):

Q: How do I use the Qiagen Viral Vector Lysis Kit in my sample preparation workflow?

A: To use the kit, simply add the provided lysis buffer to your viral vector sample according to the protocol insert. Incubate as directed to achieve complete lysis, then proceed with downstream nucleic acid purification. The kit is designed for ease of use in both manual and automated workflows.

Q: What types of viral vectors can this kit efficiently lyse?

A: The kit effectively lyses a variety of viral vectors commonly used in cell and gene therapy, including adeno-associated virus (AAV), lentivirus, adenovirus, and other recombinant viral formats.

Q: When should this kit be used during the nucleic acid purification process?

A: The kit is intended for the initial sample preparation step-cell and viral lysis-immediately before nucleic acid extraction from viral vectors in research applications.

Q: Where should I store the Qiagen Viral Vector Lysis Kit for optimal shelf life?

A: Store the kit at 2-8C as specified in the protocol to maintain reagent stability and ensure the 12-month shelf life from the manufacture date.

Q: What makes this kit beneficial for researchers working in cell and gene therapy applications?

A: Researchers benefit from the kit's optimized lysis buffers, which maximize nucleic acid yield and purity, are compatible with various viral vectors, and streamline sample preparation for reliable and reproducible results.

Q: Can the kit be used for diagnostic or clinical purposes?

A: No, this kit is strictly for research use only and is not intended for diagnostic or clinical procedures.

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