Qiagen Type-it HRM PCR Kit (400)

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Product Details:

Shelf Life 1 year (if stored as recommended)
Function Enables precise melting curve analysis after real-time PCR
Accuracy High genotyping and methylation analysis accuracy
Storage Instructions Store at -20C
Usage Type Laboratory
Measurement Range Dependent on DNA input and detection system
Features Optimized for HRM, high specificity, reproducible results, suitable for multiplex PCR
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Qiagen Type-it HRM PCR Kit (400) Price And Quantity

Qiagen Type-it HRM PCR Kit (400) Product Specifications

Power Source Not applicable (used with external PCR instrument)
Measurement Range Dependent on DNA input and detection system
Function Enables precise melting curve analysis after real-time PCR
Operating Type Automated/Manual PCR Thermocycler Compatible
Technology HRM PCR (High Resolution Melting Polymerase Chain Reaction)
Shelf Life 1 year (if stored as recommended)
Condition New
Real-Time Operation Yes
Dimension (L*W*H) Not applicable (supplied in reagent kit form)
Accuracy High genotyping and methylation analysis accuracy
Features Optimized for HRM, high specificity, reproducible results, suitable for multiplex PCR
Color Clear/Transparent (reagents)
Equipment Type High Resolution Melting (HRM) PCR Kit
Material Nucleic Acid Reagents, PCR Enzymes, Buffer Solution
Instruments Type PCR Reagent Kit
Wall Mounted No
Usage Type Laboratory
Use Detection and analysis of genetic variations, SNP genotyping, methylation analysis
Portable No
Storage Instructions Store at -20C

Product Description

Brand Qiagen

Product Type PCR Kit

Pack Size 400 x 25 ?l

CAT No 206544

Application Molecular Reagent

The Type-it HRM PCR Kit provides a reliable solution for fast and accurate genotyping via HRM analysis. The kit is optimized to even enable successful analysis of genomic loci that are difficult to amplify. The kit is highly suitable for mutation scanning and can be used to screen samples for unknown mutations. Development of new HRM genotyping assays is easy and straightforward. Specific amplification products, reduced nonspecific amplification,and reliable results are consistently ensured time-consuming optimization of PCR parameters is no longerrequired. This leads to standardization and flexibility, in addition to substantial time and cost savings. The kit is compatible with all real-time instruments suitable for HRM analysis, such as the Rotor-Gene Q,the Rotor-Gene 6000, the LightCycler 480, and the Applied Biosystems 7500 Fast System.

Advanced Genotyping and Mutation Detection

Designed for high-precision research, this kit is optimized for applications like SNP genotyping, mutation scanning, and methylation analysis. Its integrated HotStart technology and reagent formulation promote high specificity and reproducibility, making it invaluable for molecular genetics laboratories.

Comprehensive and Compatible

The kit suits a variety of real-time PCR cyclers with HRM capabilities, simplifying integration into existing workflows. It is formulated for up to 25 l reaction volumes per well, and includes enhancer and control DNA to ensure each run's accuracy. Detailed cycling protocols are provided for consistent results.

Reliable Performance and Storage

All components are non-hazardous and easy to handle under standard laboratory conditions. When stored at -20C, the kit maintains stability for up to one year, offering reliability and convenience for routine and high-throughput operations in genetic research environments.

FAQ's of Qiagen Type-it HRM PCR Kit (400):

Q: How does the Qiagen Type-it HRM PCR Kit enhance genetic variation detection?

A: The kit utilizes HRM PCR technology, providing high-resolution melting curve analysis that enables sensitive and accurate detection of genetic variations such as SNPs, mutations, and methylation changes after real-time PCR.

Q: What instruments are compatible with this HRM PCR kit?

A: The kit is compatible with real-time PCR cyclers capable of HRM analysis. Ensure your instrument supports both real-time data acquisition and high-resolution melting protocols for optimal performance.

Q: When should I use this kit for my research?

A: Use this kit when your experiments require precise genotyping, mutation scanning, methylation analysis, or robust quality control of nucleic acid samples. It is suitable for both routine and specialized molecular genetics applications.

Q: Where should the Qiagen Type-it HRM PCR Kit be stored?

A: Store all reagents at -20C immediately upon receipt. Proper storage ensures stability and consistent performance across the kit's shelf life of one year.

Q: What is the recommended process for setting up an HRM PCR reaction with this kit?

A: Follow the protocol provided with the kit: prepare a master mix using the supplied 2x HRM PCR Master Mix, HRM Enhancer, and control DNA, adjust to a final reaction volume up to 25 l per well, and use the optimized cycling conditions on a compatible real-time HRM PCR instrument.

Q: How does the HotStart technology benefit my experiments?

A: The integrated HotStarTaq Plus DNA Polymerase prevents non-specific amplification before the initial PCR activation step, enhancing specificity, and yielding clean, reproducible data, especially valuable in HRM assays.

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