Thermo Fisher Scientific Invitrogen Platinum SuperFi II PCR Master Mix

Thermo Fisher Scientific Invitrogen Platinum SuperFi II PCR Master Mix

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Product Details:

Features Ultra-high fidelity, room temperature stable, ready-to-use, hot-start mechanism, robust performance
Usage Type Laboratory reagent
Accuracy Ultra-high fidelity PCR amplification
Shelf Life 12 months (at -20C)
Instruments Type PCR reagent
Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Function DNA amplification for molecular biology applications
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Thermo Fisher Scientific Invitrogen Platinum SuperFi II PCR Master Mix Price And Quantity

Enzyme Platinum SuperFi II DNA Polymerase
Fidelity >300X higher than Taq DNA polymerase
Application Cloning, mutagenesis, sequencing, gene expression
Reaction Setup Ready-to-use master mix format
Temperature Stability Stable at room temperature for up to 24 hours
Format 2X Master Mix
Package Size Varies (e.g. 100 reactions, 500 reactions)
Hot Start Yes, antibody-mediated
Product Name Platinum SuperFi II PCR Master Mix
MgCl2 Concentration 4 mM (when used at 1X)
Sample Types Genomic DNA, cDNA, plasmid, lambda DNA
dNTP Concentration 0.4 mM each (when used at 1X)
PCR Product Size Range Up to 20 kb (genomic DNA), up to 40 kb (lambda DNA)

Thermo Fisher Scientific Invitrogen Platinum SuperFi II PCR Master Mix Product Specifications

Wall Mounted No
Use PCR amplification of DNA
Power Source Not required (used inside thermal cycler)
Color Clear solution
Function DNA amplification for molecular biology applications
Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Instruments Type PCR reagent
Equipment Type PCR Master Mix
Shelf Life 12 months (at -20C)
Material Enzyme mix, buffer, dNTPs, MgCl2
Operating Type Manual pipetting
Technology SuperFi DNA polymerase-based high-fidelity PCR
Real-Time Operation Compatible with real-time PCR
Features Ultra-high fidelity, room temperature stable, ready-to-use, hot-start mechanism, robust performance
Accuracy Ultra-high fidelity PCR amplification
Portable Yes, in tube format
Condition New
Usage Type Laboratory reagent

Enzyme Platinum SuperFi II DNA Polymerase
Fidelity >300X higher than Taq DNA polymerase
Application Cloning, mutagenesis, sequencing, gene expression
Reaction Setup Ready-to-use master mix format
Temperature Stability Stable at room temperature for up to 24 hours
Format 2X Master Mix
Package Size Varies (e.g. 100 reactions, 500 reactions)
Hot Start Yes, antibody-mediated
Product Name Platinum SuperFi II PCR Master Mix
MgCl2 Concentration 4 mM (when used at 1X)
Sample Types Genomic DNA, cDNA, plasmid, lambda DNA
dNTP Concentration 0.4 mM each (when used at 1X)
PCR Product Size Range Up to 20 kb (genomic DNA), up to 40 kb (lambda DNA)

Product Description

Brand	Thermo Fisher Scientific
Product Type	PCR Master Mix
Pack Size	500T
CAT No	12368050
Application	Molecular Biology

Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification. Platinum SuperFi II DNA Polymeraseis a proofreading DNA polymerase that combines superior fidelity with innovative SuperFi II Buffer, enabling universal primer annealing for the highest success in PCR. It is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Ultra-High Fidelity PCR Amplification

Leveraging Platinum SuperFi II DNA Polymerase, this master mix achieves greater than 300X fidelity compared to conventional Taq polymerase, making it ideal for cloning, mutagenesis, and sequencing where accuracy is paramount. Its reliable amplification supports multiple DNA templates, ensuring consistent results even for complex genetic targets.

Convenient, Ready-to-Use Format

Supplied as a 2X master mix, Platinum SuperFi II simplifies reaction setup and minimizes pipetting errors. The hot-start mechanism, facilitated by antibody-mediated inhibition, enhances specificity while the room temperature stability for 24 hours allows flexible workflow integration in busy labs.

Versatile Applications and Compatibility

Engineered for use with genomic DNA, cDNA, plasmid, and lambda DNA, this PCR master mix excels in molecular cloning, mutagenesis, gene expression, and sequencing studies. It supports both endpoint and real-time PCR platforms, making it a valuable reagent in research and diagnostic laboratories.

FAQ's of Thermo Fisher Scientific Invitrogen Platinum SuperFi II PCR Master Mix:

Q: How should I store Platinum SuperFi II PCR Master Mix for optimal performance?

A: Platinum SuperFi II PCR Master Mix must be stored at -20C to maintain enzyme activity and longevity. It is recommended to avoid repeated freeze-thaw cycles, as these can reduce performance and fidelity.

Q: What types of DNA samples are compatible with this master mix?

A: This master mix is suitable for various sample types, including genomic DNA, cDNA, plasmid DNA, and lambda DNA. Its robust formulation allows efficient amplification across a breadth of molecular biology protocols.

Q: When can I benefit from the hot-start feature of this PCR master mix?

A: The antibody-mediated hot-start feature prevents non-specific amplification and primer-dimer formation during reaction setup. This is particularly beneficial if you need improved specificity or are working with complex templates.

Q: Where can the Platinum SuperFi II PCR Master Mix be used in the laboratory?

A: Platinum SuperFi II PCR Master Mix is designed for laboratory use, ideal for applications such as gene cloning, site-directed mutagenesis, and sequencing, as well as gene expression research and other molecular diagnostics.

Q: What process should I follow to set up a PCR reaction using this master mix?

A: To set up a PCR reaction, simply combine the master mix with your template DNA and primers according to the recommended volumes for each reaction. Its 2X formulation means minimal pipetting steps, simplifying your workflow.

Q: How does the ultra-high fidelity of the Platinum SuperFi II benefit my experiments?

A: The superior fidelity-over 300 times greater than Taq polymerase-ensures accurate DNA amplification with minimal errors, which is essential for applications where sequence accuracy is critical, such as cloning and mutagenesis.

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