Product Description
Brand BioRad
Product Type DNA Kit
Pack Size 450 l
CAT No 4561021
Application Molecular Biology
Use these Mini-PROTEAN TGX Precast Protein Gels with IPG/prep wells for either preparative electrophoresis or as the second dimension after isoelectric focusing (IEF). Long-life TGX (Tris-Glycine eXtended) Gels have a novel formulation and can be used for both standard denaturing protein separations as well as native electrophoresis.
High Capacity and ConsistencyEach Mini-PROTEAN TGX gel features a 7 cm IPG/prep well capable of holding up to 450 l, supporting preparative or analytical sample loading. The TGX chemistry ensures consistent performance, high-resolution separation in the 15-100 kD range, and reproducible results across experiments.
Rapid and Reliable ElectrophoresisWith a typical electrophoresis run time of around 35 minutes, these gels streamline laboratory workflows. The ready-to-use polyacrylamide format eliminates the need for casting, while the gels remain stable for 12 months if properly stored at 2-8C (do not freeze), offering convenience for routine usage.
Seamless Compatibility and Easy StorageDesigned to fit with the Bio-Rad Mini-PROTEAN Tetra Cell and similar electrophoresis systems, these gels are portable, easy to handle, and supplied in clear cassettes for straightforward use. Their durability and clear labeling make them the trusted choice for laboratories across India and globally.
FAQ's of BioRad 7.5% Mini-PROTEAN TGX Precast Protein Gels, 7 cm IPG/prep well, 450 ul:
Q: How should I store BioRad 7.5% Mini-PROTEAN TGX Precast Protein Gels?
A: Store the gels at 2-8C and avoid freezing to maintain their stability and performance throughout their 12-month shelf life.
Q: What is the maximum sample volume per prep well for these gels?
A: Each 7 cm IPG/prep well can accommodate up to 450 l, allowing for preparative or larger sample analysis.
Q: When is it optimal to use a 7.5% gel for protein separation?
A: A 7.5% gel is ideal for resolving proteins in the 15-100 kD molecular weight range, though it can be used for a broader range with some compromise in resolution.
Q: Where can these gels be used, and with which instruments are they compatible?
A: These gels are designed for laboratory use and are compatible with Bio-Rad's Mini-PROTEAN Tetra Cell and other similar vertical electrophoresis systems.
Q: What benefits do the TGX Precast Protein Gels provide over traditional hand-cast gels?
A: TGX Precast gels offer ready-to-use convenience, consistent results, enhanced shelf life, and eliminate the variability and labor associated with hand-casting gels.
Q: How long does a typical electrophoresis run take using these gels?
A: A standard SDS-PAGE run on these gels takes approximately 35 minutes under typical conditions.
Q: What is the primary application or use for these Mini-PROTEAN TGX gels?
A: These gels are mainly used for the separation and analysis of proteins by SDS-PAGE in research laboratories.