Product Description
| Brand |
Thermo Fisher Scientific |
| Product Type |
RNA Kit |
| Pack Size |
96 Preps |
| CAT No |
AM1830 |
| Application |
Molecular Biology |
The MagMAX-96 Total RNA Isolation Kit is designed for the rapid, moderate-throughput purification of RNA from animal and plant cells and tissue. RNA purified using the kit is ideal for PCR and RT-PCR applications. The kit uses magnetic beadabased purification and includes sufficient reagents for 96 reactions.
High-Throughput RNA ExtractionProcess up to 96 samples simultaneously with the MagMAX-96 kit, ideal for laboratories requiring reliable, large-scale RNA isolation. The workflow is designed for efficient and rapid processing, significantly saving time in high-demand research environments.
Versatile Sample CompatibilityThe kit supports total RNA purification from a wide variety of biological sources including cultured cells, tissues, bacteria, and blood. This flexibility makes it an excellent choice for researchers handling diverse sample types in their workflows.
Optimized for Sensitive ApplicationsAchieve typically over 90% nucleic acid recovery, delivering high-purity RNA suitable for sensitive downstream applications such as qPCR, RT-PCR, next-generation sequencing (NGS), and microarrays. Optional DNase treatment can be added for DNA-free RNA preparations.
Streamlined Protocols and Convenient SetupThis kit's magnetic bead technology and inclusion of ready-to-use reagents allow for a straightforward workflow. It is compatible with both manual and automated 96-well formats, providing versatility for various research and clinical laboratory settings.
FAQ's of Thermo Fisher Scientific Invitrogen MagMAX-96 Total RNA Isolation Kit:
Q: How do I use the MagMAX-96 Total RNA Isolation Kit for different sample types?
A: Follow the included protocol, which details sample preparation steps for cultured cells, tissues, bacteria, blood, and other biological materials. Adjust input volumes accordingly (1-10^6 cells or 20-200 l per well) for optimal RNA yield.
Q: What downstream applications are compatible with RNA purified using this kit?
A: RNA purified with the MagMAX-96 kit is of high purity and integrity, making it suitable for sensitive applications such as qPCR, RT-PCR, next-generation sequencing (NGS), and microarrays.
Q: When should I perform optional DNase treatment during the RNA isolation process?
A: You may include DNase treatment after the initial extraction if you need to remove residual genomic DNA. DNase is not included in the kit and must be supplied separately.
Q: Where should I store the kit and its reagents?
A: Store the entire MagMAX-96 Total RNA Isolation Kit at 2-8C as recommended to preserve reagent stability and kit performance throughout its one-year shelf life.
Q: What is the typical extraction time and throughput for this kit?
A: The MagMAX-96 kit enables rapid extraction, processing up to 96 samples in approximately 45 minutes, making it suitable for laboratories that require high-throughput workflows.
Q: How does the magnetic bead-based technology benefit RNA isolation?
A: Magnetic bead technology ensures efficient capture and purification of RNA, resulting in high recovery rates (usually over 90%) and high-purity RNA suitable for sensitive and quantitative downstream experimentation.
Q: Can I use this kit with automated liquid handlers or must I process samples manually?
A: The kit is compatible with both manual workflows and automated liquid handling robots in a 96-well format, providing flexibility based on laboratory resources and throughput needs.