Qiagen QIAamp MinElute ccfDNA Midi Kit (50)

Qiagen QIAamp MinElute ccfDNA Midi Kit (50)

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Product Details:

Features Efficient and rapid recovery of ccfDNA, Processing of plasma volumes up to 8 ml per column, Minimal hands-on time, MinElute spin columns for elution in small volumes
Instruments Type DNA extraction kit
Accuracy High-yield recovery of pure ccfDNA suitable for downstream applications
Shelf Life As indicated on label (typically up to 1 year)
Usage Type Laboratory
Storage Instructions Store at room temperature (15-25C); Proteinase K should be stored at 2-8C on arrival
Measurement Range Up to 8 ml plasma per sample
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Qiagen QIAamp MinElute ccfDNA Midi Kit (50) Price And Quantity

Qiagen QIAamp MinElute ccfDNA Midi Kit (50) Product Specifications

Technology Silica-based membrane technology (spin column)
Function Purification of circulating cell-free DNA for research or diagnostic applications
Measurement Range Up to 8 ml plasma per sample
Use Extraction and purification of circulating cell-free DNA (ccfDNA) from human plasma
Wall Mounted No
Real-Time Operation No
Storage Instructions Store at room temperature (15-25C); Proteinase K should be stored at 2-8C on arrival
Accuracy High-yield recovery of pure ccfDNA suitable for downstream applications
Usage Type Laboratory
Shelf Life As indicated on label (typically up to 1 year)
Condition New
Equipment Type ccfDNA Extraction Kit
Portable No
Instruments Type DNA extraction kit
Material Reagents and consumables for nucleic acid purification
Features Efficient and rapid recovery of ccfDNA, Processing of plasma volumes up to 8 ml per column, Minimal hands-on time, MinElute spin columns for elution in small volumes
Color White/Transparent (kit components)
Operating Type Manual

Product Description

Brand Qiagen

Product Type DNA Extraction Kit

Pack Size 50T

CAT No 55284

Application Molecular Reagent

QIAamp MinElute ccfDNA Kits greatly simplify concentration and purification of free-circulating DNA from plasma or serum. The procedure can be partially automated on the QIAcube Connect.

High-Efficiency ccfDNA Extraction

The QIAamp MinElute ccfDNA Midi Kit maximizes DNA recovery from plasma volumes up to 8 ml, providing researchers with sufficient amounts of high-quality cell-free DNA for diverse molecular applications. The process is designed for ease of use, with minimal hands-on time and rapid processing.

Seamless Compatibility with Downstream Applications

This kit ensures the extracted ccfDNA is of exceptional purity, making it well-suited for sensitive applications such as PCR, qPCR, and Next Generation Sequencing (NGS). The streamlined workflow enables straightforward integration into existing laboratory protocols.

Reliable Performance and Convenience

With manual operating type and easy-to-follow instructions, the MinElute spin columns allow for elution volumes as low as 20 l, maximizing DNA concentration. Provided reagents, as well as specified storage conditions, ensure consistent results throughout the kit's shelf life.

FAQ's of Qiagen QIAamp MinElute ccfDNA Midi Kit (50):

Q: How does the QIAamp MinElute ccfDNA Midi Kit extract DNA from plasma samples?

A: The kit utilizes silica-based membrane spin columns, which selectively bind circulating cell-free DNA from plasma. After binding, contaminants are washed away and pure ccfDNA is eluted in low volumes, using the included buffers and reagents.

Q: What plasma sample volumes can be processed with this kit?

A: You can process between 1 ml and 8 ml of human plasma per preparation, providing flexibility for varying sample inputs and maximizing DNA yield as needed for your study.

Q: When should I use the QIAamp MinElute ccfDNA Midi Kit over other extraction kits?

A: This kit is ideal when you need high-yield, high-purity ccfDNA from larger plasma volumes, especially for applications like PCR, qPCR, or NGS studies. Its ability to process up to 8 ml per sample and elute in small volumes is particularly beneficial for low-abundance DNA targets.

Q: Where can I use the QIAamp MinElute ccfDNA Midi Kit?

A: The kit is intended for laboratory use only and must be handled by trained personnel for research purposes. It is not to be used for diagnostic procedures.

Q: What are the main steps involved in the ccfDNA extraction process with this kit?

A: The workflow includes plasma pre-treatment with Proteinase K and buffers, binding the DNA to the MinElute column, washing contaminants away, and then eluting the purified DNA in as little as 20 l. The entire process takes about one hour per sample.

Q: How should the kit and its contents be stored?

A: Store the kit components at room temperature (15-25C). Upon arrival, store Proteinase K at 2-8C to maintain its stability. Always refer to the label for detailed storage instructions and shelf life information.

Q: What are the benefits of using the QIAamp MinElute ccfDNA Midi Kit for ccfDNA extraction?

A: The kit provides efficient and rapid recovery of cell-free DNA with minimal hands-on time, optimal processing of plasma volumes, and high purity of DNA, ensuring reliable results in PCR, qPCR, and NGS research applications.

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