Product Description
Brand Qiagen
Product Type RNA Kit
Pack Size 12 x 96 Wells
CAT No 74182
Application Molecular Reagent
RNeasy Kits are the gold standard for total RNA isolation. They provide fast purification of high-quality RNA from small to large amounts of cells, tissues, and yeast using silica membrane RNeasy spin columns or 96-well plates. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor II or TissueLyser II or LT system. The RNeasy 96 Kit enables high-throughput purification of total RNA from up to 96 cultured cell samples using silica membrane RNeasy 96 plates. A dedicated RNeasy QIAcube Kit enables automated purification of 112 samples on the QIAcube Connect.
High-Throughput RNA PurificationWith the Qiagen RNeasy 96 Kit, laboratories can process up to 96 samples simultaneously in a single run. This efficiency is ideal for studies requiring large-scale or parallel RNA extractions, offering speed and uniformity without compromising yield or purity.
Versatile Sample CompatibilityThis kit is suitable for various research contexts, supporting the extraction of total RNA from animal cells, tissues, yeast, and bacteria. Its adaptability makes it a favored option for multidisciplinary research teams, streamlining the workflow for diverse sample types.
Consistent and Reliable ResultsUtilizing proven silica membrane technology, the RNeasy 96 Kit consistently delivers high-quality RNA with A260/A280 ratios around 2.0. The high reproducibility and ready-to-use reagents enable researchers to achieve trustworthy results, experiment after experiment.
FAQ's of Qiagen RNeasy 96 Kit (12):
Q: How does the Qiagen RNeasy 96 Kit streamline the RNA purification process?
A: The RNeasy 96 Kit enables simultaneous processing of up to 96 samples in a 96-well plate format, significantly increasing throughput and reducing hands-on time compared to single-tube extraction methods.
Q: What types of samples can be processed with this kit?
A: The kit supports total RNA isolation from a wide range of sample types, including animal cells, tissues, yeast, and bacteria, making it suitable for varied research applications.
Q: When should I use the RNeasy 96 Kit in my laboratory workflow?
A: The kit is ideal when high-throughput RNA extraction from multiple samples is needed, such as gene expression studies, RNA profiling, or other research requiring consistent, high-quality RNA yields.
Q: Where is the Qiagen RNeasy 96 Kit best utilized?
A: This kit is designed for use in research laboratories that require efficient, parallel RNA processing-especially those equipped with compatible centrifuges, vacuum manifolds, or certain robotic systems.
Q: What is the typical RNA yield per well with this kit?
A: Depending on sample type and input amount, the kit can yield up to 10 g of total RNA per well with elution volumes ranging from 60-120 l.
Q: How do I ensure proper storage and shelf life for the kit?
A: For optimal performance and to maintain a 12-month shelf life, store the kit components at room temperature (15-25C) as specified in the user manual.
Q: What are the main benefits of using the RNeasy 96 Kit for RNA isolation?
A: The kit provides rapid, reproducible results with high RNA purity, ready-to-use reagents, and scalability for high-throughput research, supporting consistent and reliable experimental outcomes.