Qiagen QIAseq FX DNA Library Kit HT A-D (384)

Qiagen QIAseq FX DNA Library Kit HT A-D (384)

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Product Details:

Features High-throughput, enzymatic fragmentation, rapid protocol, high yield, compatible with Illumina platforms
Function Simultaneous DNA fragmentation and library prep
Accuracy High fidelity, minimizes sequence bias
Storage Instructions Store at -20C
Shelf Life 12 months from manufacturing date
Usage Type Laboratory use
Measurement Range Compatible with DNA input 10 ng - 1 g
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Qiagen QIAseq FX DNA Library Kit HT A-D (384) Price And Quantity

Qiagen QIAseq FX DNA Library Kit HT A-D (384) Product Specifications

Measurement Range Compatible with DNA input 10 ng - 1 g
Use Preparation of DNA libraries for Next-Generation Sequencing
Condition New
Portable Yes
Color Multi-color (as per reagent)
Operating Type Manual
Usage Type Laboratory use
Shelf Life 12 months from manufacturing date
Features High-throughput, enzymatic fragmentation, rapid protocol, high yield, compatible with Illumina platforms
Storage Instructions Store at -20C
Weight Approx. 1 kg (shipping weight)
Power Source Not required (compatible with common laboratory thermal cycler)
Wall Mounted No
Technology Enzymatic Fragmentation and Library Prep (NGS)
Material Reagents, Enzyme Mix, Buffers, Solution, Tubes
Dimension (L*W*H) Kit dimensions vary; standard laboratory kit size
Accuracy High fidelity, minimizes sequence bias
Real-Time Operation No
Instruments Type NGS Library Preparation Kit
Equipment Type DNA Library Preparation Kit
Function Simultaneous DNA fragmentation and library prep

Input DNA Quality High molecular weight genomic DNA
PCR-free Option No
Sample Throughput High-throughput 384-sample format (HT A-D 384)
Fragmentation Method Proprietary enzymatic fragmentation
Typical Workflow Time ~2 hours
Barcode Support Index/adapter/barcoding compatible
Kit Compatibility Illumina sequencing platforms
Product Code 180475
Application Area Human, animal, plant, microbial genomics, and metagenomics
Plate Format 4 x 96-well plates
Hazard Information Non-hazardous components, MSDS available on request
Pack Size 384 reactions (HT A-D plates included)

Product Description

Brand Qiagen

Product Type PCR Kit

Pack Size 384 Wells

CAT No 180487

Application Molecular Reagent

Our QIAseq FX technology incorporates an all-enzymatic DNA fragmentation into a streamlined, optimized protocol that doesnt require additional sample clean-up before adapter ligation. A simple, three-reaction protocol enables straightforward automation of library preparation on various liquid-handling platforms, reducing hands-on time and run-to-run variability.

Efficient High-Throughput Library Preparation

This kit streamlines DNA library preparation for next-generation sequencing, accommodating 384 samples in a single run. The process is optimized for laboratories demanding efficiency, scalability, and reproducibility, facilitating research in genomics and metagenomics across multiple domains-from human and animal studies to plant and microbial investigations.

Advanced Enzymatic Fragmentation

By leveraging proprietary enzymatic technology, the kit enables highly accurate and uniform library construction. This method reduces sequence bias and ensures consistent results, which is critical for downstream applications where reproducibility and data integrity are essential.

User-Friendly Format and Compatibility

Packed as four 96-well plates, the kit provides a flexible and organized solution, seamlessly integrating with common laboratory workflows. It is fully compatible with Illumina sequencing systems, includes indexing/barcode options, and supports a wide DNA input range, making it suitable for various project scales.

FAQ's of Qiagen QIAseq FX DNA Library Kit HT A-D (384):

Q: How do I use the QIAseq FX DNA Library Kit HT A-D (384) in my laboratory workflow?

A: The kit is designed for manual use in standard laboratory settings. Simply add your high molecular weight DNA samples into the 96-well plates provided, follow the included protocol for enzymatic fragmentation and library preparation, and proceed with sample indexing and clean-up before sequencing.

Q: What are the main benefits of this library preparation kit for high-throughput sequencing projects?

A: This kit allows rapid preparation of up to 384 DNA libraries in under 2 hours, maintains high accuracy with minimal sequence bias, and is compatible with diverse DNA sources. Its scalability and efficiency reduce hands-on time and maximize laboratory productivity.

Q: When should I choose this kit for my DNA sequencing project?

A: Select this kit when you require high-throughput processing, especially if you are working with large sample sets or requiring quick turnaround. It is particularly suitable for human, animal, plant, microbial, and metagenomics research that uses Illumina platforms.

Q: What DNA input quality and quantity are required for optimal results?

A: The kit is optimized for high molecular weight genomic DNA with input amounts ranging from 10 ng to 1 g per reaction. Using high-quality DNA ensures efficient enzymatic fragmentation and consistent library yields.

Q: Where should the kit be stored, and what is its shelf life?

A: Store the QIAseq FX DNA Library Kit HT A-D (384) at -20C to maintain its integrity. The kit has a shelf life of 12 months from the manufacturing date when stored appropriately.

Q: Is the kit safe to use, and are there any hazardous components?

A: The components are classified as non-hazardous, and the kit is safe for standard laboratory use. Material Safety Data Sheets (MSDS) are available upon request for your records or compliance needs.

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