Product Description
| Brand |
Thermo Fisher Scientific |
| Product Type |
RNA Mini Kit |
| Pack Size |
250 Preps |
| CAT No |
12183025 |
| Product Class |
Molecular Biology |
The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment. The kit includes RNase-free lysis and wash solutions that protect RNA from RNases while liberating the RNA from DNA, proteins, and other cellular debris. The fast spin-column workflow is ideal for processing low to mid-throughput batch sizes. The advanced PureLink RNA Mini spin column design allows for maximum sample input (200 mg of tissue) and RNA recovery (up to 1000 I14g). This means that users can process both small and large sample sizes with the same RNA isolation kit.
Versatile RNA Isolation for Various SamplesThe PureLink RNA Mini Kit is adaptable for isolating RNA from diverse biological samples such as animal tissues, cultured cells, blood, bacteria, and yeast, including specimens stored in RNAlater. Its broad compatibility makes it an ideal tool for laboratories working with multiple sample types in gene expression and molecular biology research.
Fast, Efficient, and High-Purity ResultsThis kit utilizes innovative silica-membrane spin column technology, allowing rapid processing and high RNA yields with minimal contamination. You can expect to process each sample in under 30 minutes, obtaining RNA with the purity and integrity required for demanding downstream applications.
FAQ's of Thermo Fisher Scientific Invitrogen PureLink RNA Mini Kit:
Q: How does the PureLink RNA Mini Kit ensure the purity and integrity of isolated RNA?
A: The kit employs silica-membrane spin column technology and RNase-free reagents to efficiently purify RNA while minimizing the risk of contamination. Each kit batch is quality controlled for RNA yield, purity, and integrity, ensuring reproducible high-quality results.
Q: What types of samples can be processed with this RNA isolation kit?
A: The kit supports a wide range of sample types, including animal tissues, cultured mammalian or bacterial cells, blood, bacteria, and yeast, as well as samples stored in RNAlater.
Q: When should I use the PureLink RNA Mini Kit in my research workflow?
A: Use this kit when you require rapid and reliable isolation of total RNA for downstream applications such as RT-PCR, qPCR, northern blotting, or microarray analysis. It is suitable for laboratories performing routine or high-throughput RNA extraction.
Q: Where do I store the kit and how long can I use it?
A: The kit should be stored at room temperature (15-30C). Under recommended conditions, it maintains optimal performance with a shelf life of 12 months from the date of manufacture.
Q: What is the typical process for isolating RNA using this kit?
A: The process involves lysing the sample with the provided buffer, binding RNA to the spin column, washing away contaminants, and eluting pure RNA in RNase-free water. The whole procedure takes less than 30 minutes per sample.
Q: How much RNA can I expect to obtain from this kit?
A: Depending on the sample type and input amount, each preparation can yield up to 100 micrograms of total RNA, with the column capable of binding up to 100 g RNA per run.
Q: What are the main benefits of using the Invitrogen PureLink RNA Mini Kit?
A: The kit is portable, easy to use, and delivers rapid, high-yield, and high-purity RNA suitable for sensitive molecular experiments. Its flexibility accommodates various sample types and ensures reliable results for research purposes.