Product Description
Brand Qiagen
Product Type PCR Kits
Pack Size 50T
CAT No 51194
Application Molecular Reagent
QIAamp DNA Blood Kits provide silica-membrane-based DNA purification from whole blood, plasma, serum and other body fluids. The kits are designed for a range of sample sizes from 200 l up to 10 ml fresh or frozen human whole blood. QIAamp spin columns can be easily processed in a centrifuge or on vacuum manifolds. A convenient 96-well format using centrifugation enables purification of DNA for labs that need high-throughput DNA purification from blood, buffy coat, plasma, serum, bone marrow, lymphocytes and body fluids. A dedicated kit is also available for automated purification of 112 samples on the QIAcube Connect.
Efficient and Fast DNA IsolationThe QIAamp DNA Blood Maxi Kit streamlines DNA purification from large blood volumes, delivering up to 400 g of DNA per prep within 40 minutes. Utilizing silica membrane technology ensures rapid processing, reducing wait times and expediting workflows for laboratories needing fast, high-purity DNA preparation.
High Yield and Purity for Reliable ResultsThis kit consistently achieves high DNA yield and purity from whole blood, plasma, or buffy coat. With an elution volume of 500-1,000 l, extracted DNA is compatible with the majority of downstream molecular biology applications, including PCR, sequencing, and blotting techniques.
Simple Manual Operation, Broad CompatibilityDesigned for manual processing, the kit requires no specialized equipment and fits seamlessly into laboratory settings. Its DNA is ready for use in a wide range of applications, making it highly versatile for research, clinical, and molecular diagnostic purposes.
FAQ's of Qiagen QIAamp DNA Blood Maxi Kit (50):
Q: How does the Qiagen QIAamp DNA Blood Maxi Kit purify DNA from blood samples?
A: This kit employs a silica-based membrane technology, which binds DNA while impurities are washed away. The process is performed manually using polypropylene columns to ensure efficient separation and reliable DNA recovery from whole blood, plasma, or buffy coat samples.
Q: What types of samples can be processed with this kit, and what is the maximum input?
A: The kit is suitable for use with whole blood, plasma, and buffy coat, allowing for up to 10 ml of whole blood per preparation, and consistently produces high yields of DNA across sample types.
Q: When should this kit be used in a laboratory workflow?
A: It is ideal for instances when high-purity, high-yield genomic DNA is needed promptly for downstream applications such as PCR, qPCR, genotyping, sequencing, or Southern blotting.
Q: Where can researchers use the DNA isolated with this kit?
A: The purified DNA is compatible with most downstream molecular biology processes-including research, diagnostic, and genetic analysis workflows-in standard laboratory environments.
Q: What is the typical processing time for a single DNA preparation?
A: One prep typically takes around 40 minutes, making it a time-efficient solution for labs requiring fast turnaround without sacrificing DNA quality.
Q: How should the kit be stored to ensure optimal performance?
A: Store the kit at room temperature (15-25C) as per the manufacturer's guidelines. Adhering to proper storage ensures a shelf life of 12-24 months.
Q: What are the key benefits of using this kit over alternative methods?
A: The kit offers extremely fast processing, high yield and purity, reliable results for sensitive applications, easy manual handling, and broad applicability across molecular biology techniques.