Qiagen EpiTect MethyLight PCR +ROX Vial Kit (200)

Qiagen EpiTect MethyLight PCR +ROX Vial Kit (200)

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Product Details:

Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Features High specificity and sensitivity, Compatible with ROX reference dye
Usage Type Laboratory
Measurement Range Quantitative detection of methylated DNA
Shelf Life 12 months (when stored at -20C)
Instruments Type Real-Time PCR compatible
Function Quantification of DNA methylation by Real-Time PCR
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Qiagen EpiTect MethyLight PCR +ROX Vial Kit (200) Price And Quantity

Qiagen EpiTect MethyLight PCR +ROX Vial Kit (200) Product Specifications

Real-Time Operation Yes
Power Source Not Required (requires compatible PCR instrument)
Technology MethyLight Real-Time PCR
Portable No
Measurement Range Quantitative detection of methylated DNA
Color White/Transparent (Reagents)
Equipment Type PCR Kit
Material Reagents and Enzymes
Display Type Not Applicable (used with PCR instrument)
Operating Type Automated
Accuracy High specificity for methylated DNA
Usage Type Laboratory
Weight Approx. 200 reactions (kit size), not applicable
Wall Mounted No
Noise Level Silent, Not Applicable
Features High specificity and sensitivity, Compatible with ROX reference dye
Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Condition New
Use DNA Methylation Analysis
Instruments Type Real-Time PCR compatible
Function Quantification of DNA methylation by Real-Time PCR
Shelf Life 12 months (when stored at -20C)

Product Description

Brand Qiagen

Product Type dPCR Kit

Pack Size 200 x 50 L

CAT No 59496

Application Molecular Reagent

The EpiTect MethyLight PCR Kit and the EpiTect MethyLight PCR+ROX Vial Kit enable sensitive and reliable quantification for TaqMan or dual-labeled probe-based methylation analysis. The kits are available in a ready-to-use format, eliminating the need for further optimization. The kits are compatible with a wide range of real-time cyclers. The EpiTect MethyLight PCR Kit is suitable for most cyclers from Applied Biosystems, with the exception of the Applied Biosystems 7500 systems. The EpiTect MethyLight PCR+ROX Vial Kit is suitable for all other cyclers, including the Applied Biosystems 7500 and instruments from Bio-Rad, Cepheid, Corbett, Eppendorf, Roche, and Stratagene.

Sensitive Quantitative Methylation Detection

The EpiTect MethyLight PCR +ROX Vial Kit is engineered for the sensitive and quantitative assessment of DNA methylation using MethyLight technology. Its high specificity ensures accurate results, even with low-abundance methylated DNA, making it ideal for research and diagnostic applications.

User-Friendly and Flexible Design

With individually packaged reagents and enzyme vials, as well as a separate ROX reference dye, this kit supports both flexibility and ease of use. The reagents are optimized for compatibility with a wide range of Real-Time PCR instruments using ROX normalization, allowing straightforward integration into your existing workflow.

Optimized for Laboratory Precision

Designed for medium to high sample throughput, the kit supports consistent and reproducible results. A dedicated buffer, reliable enzyme formulation, and clear storage at -20C contribute to assay stability and performance throughout the kit's 12-month shelf life.

FAQ's of Qiagen EpiTect MethyLight PCR +ROX Vial Kit (200):

Q: How should the Qiagen EpiTect MethyLight PCR +ROX Vial Kit be stored for optimal performance?

A: Store all kit components at -20C and avoid repeated freeze-thaw cycles to maintain reagent quality over the 12-month shelf life.

Q: What type of DNA template is compatible with this kit?

A: This kit is specifically designed for use with bisulfite-converted DNA, ensuring precise detection and quantification of DNA methylation levels.

Q: When is the ROX reference dye used in the protocol?

A: The ROX reference dye is used during Real-Time PCR runs for normalization purposes. Add it as instructed for your compatible instrument to ensure accurate quantitative analysis.

Q: Where can the EpiTect MethyLight PCR +ROX Kit be used?

A: This kit is intended for laboratory use, specifically in settings requiring sensitive and automated DNA methylation analysis using Real-Time PCR technology.

Q: What is the process for DNA methylation quantification using this kit?

A: Follow a standard MethyLight Real-Time PCR protocol: prepare your bisulfite-converted DNA, mix with the PCR Master Mix, buffer, and ROX dye, then run your samples on a compatible Real-Time PCR instrument.

Q: What are the main benefits of using this kit for methylation analysis?

A: The kit offers high specificity and sensitivity for methylated DNA detection, supports medium to high sample throughput, and is compatible with most Real-Time PCR instruments utilizing ROX normalization.

Q: How many reactions are supported, and what is the sample throughput?

A: The kit supports up to 200 reactions per box, making it suitable for laboratories with medium to high-throughput requirements.

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