Product Description
Brand Qiagen
Product Type PCR Kits
Pack Size 1000 mL
CAT No 158026
Application Molecular Reagent
Puregene Kits enable purification of high-molecular-weight (100 200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows samples to be purified for use as long-term references.
Efficient DNA Extraction for Molecular ApplicationsWith the Qiagen Puregene Blood Kit, researchers can consistently isolate pure genomic DNA from a range of sample types, including whole blood, buffy coat, and cultured cells. Its robust protocol ensures high yields and purity, making it an excellent choice for various molecular biology, genomics, and clinical research applications. Each kit streamlines workflow with ready-to-use reagents and supports scalability for small to high-throughput laboratories.
User-Friendly and Reliable PerformanceThis kit is designed to simplify DNA extraction processes without compromising results. The included buffers facilitate each step, from cell lysis to DNA hydration, ensuring reproducible outcomes. It is compatible with manual handling and requires no specialized equipment, making it suitable for diverse research settings. Silent in operation and portable, it integrates smoothly into laboratory environments.
FAQ's of Qiagen Puregene Blood Kit (1000 ml):
Q: How can the Qiagen Puregene Blood Kit be used to extract DNA from blood samples?
A: The kit employs a silica-membrane spin column technique to isolate genomic DNA. Simply follow the manual steps: lyse cells, precipitate proteins, wash with supplied buffers, and hydrate DNA, resulting in high-purity DNA suitable for downstream applications.
Q: What sample types are compatible with the Puregene Blood Kit?
A: The kit is designed to process whole blood, buffy coat, and cultured cells, making it versatile for various laboratory and clinical samples.
Q: When should the kit be stored and how long is its shelf life?
A: Store the kit at 2-25C, away from direct light. When stored properly, reagents retain performance for 12-18 months from the date of manufacture.
Q: Where is this kit most commonly utilized?
A: It is widely used in research and diagnostics laboratories for molecular biology, genomics, biobanking, and clinical research applications requiring high-quality DNA isolation.
Q: What are the typical DNA yields and measurement range per sample?
A: From 4 ml of blood, the kit typically yields 10-50 g of DNA. It is suitable for sample volumes ranging from 0.3 to 10 ml per preparation.
Q: What benefits does the Puregene kit offer researchers?
A: The kit provides high yield and consistent purity of DNA, supports a scalable number of preps per kit, and ensures the DNA meets quality demands for sensitive downstream analyses.
Q: What is included in the kit and what are the main steps of the process?
A: The kit contains all required buffer solutions: cell lysis, protein precipitation, DNA hydration, and washing buffers. The process involves sample lysis, protein removal, DNA binding to the membrane, washing, and final DNA hydration.