Thermo Scientific ABsolute QPCR Mix, low ROX

Thermo Scientific ABsolute QPCR Mix, low ROX

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Product Details:

Function Enables amplification and real-time detection in qPCR
Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Instruments Type qPCR instruments compatible with low ROX
Shelf Life One year from date of manufacture (when stored correctly)
Accuracy High (for qPCR quantification)
Measurement Range Wide dynamic range (up to 8 logs)
Usage Type Laboratory/Research
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Thermo Scientific ABsolute QPCR Mix, low ROX Price And Quantity

Thermo Scientific ABsolute QPCR Mix, low ROX Product Specifications

Portable Yes
Function Enables amplification and real-time detection in qPCR
Use For quantitative PCR detection of DNA and RNA targets
Accuracy High (for qPCR quantification)
Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Dimension (L*W*H) Not applicable (supplied in vials/tubes)
Condition New
Material Aqueous solution containing dNTPs, buffer, and proprietary enhancers
Color Clear/Colorless solution
Instruments Type qPCR instruments compatible with low ROX
Technology Real-Time PCR (qPCR)
Noise Level Silent (not applicable)
Wall Mounted No
Real-Time Operation Yes
Measurement Range Wide dynamic range (up to 8 logs)
Shelf Life One year from date of manufacture (when stored correctly)
Weight Variable, as per vial size (commonly 1.25 mL or 5 mL formats)
Equipment Type qPCR Mix
Usage Type Laboratory/Research
Power Source Not required (used as a reagent)
Operating Type Manual pipetting (for reaction setup)
Features Low ROX for compatibility with a range of real-time PCR systems, high specificity and sensitivity

Detection Chemistry SYBR Green or probe-based assays
Template Compatibility DNA and cDNA
Format Ready-to-use 2X Master Mix
ROX Reference Dye Concentration Low ROX formulation
Enzyme Included Thermostable DNA polymerase
Reaction Volume Recommended 1050 L
Application Gene expression analysis, pathogen detection, genotyping
Catalog Number AB-1158/A
Barcode Yes (if required for inventory control)

Product Description

Brand	Thermo Fisher Scientific
Product Type	QPCR Mix
Pack Size	400 Reactions
CAT No	AB1319A
Application	Molecular Biology

Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility. ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye. There is also a master mix version optimized for capillary instruments.

High Sensitivity and Broad Compatibility

ABsolute QPCR Mix, low ROX, is engineered to deliver precise quantification across a wide dynamic range, suitable for DNA and cDNA templates. Its low ROX dye formulation allows the mix to be used with multiple real-time PCR systems, maximizing flexibility in laboratory workflows.

Efficient and Convenient Workflow

The 2X ready-to-use format of this master mix streamlines reaction setup, minimizing pipetting steps and ensuring consistency between samples. The mix contains all necessary components for amplification, requiring only the addition of primers, probe, and template, reducing manual errors.

Applications in Modern Molecular Research

This qPCR mix supports a variety of research applications, including gene expression profiling, genetic typing, and pathogen identification. Its robust performance makes it a valuable tool for laboratories engaged in genomics, diagnostics, or biotechnology research.

FAQ's of Thermo Scientific ABsolute QPCR Mix, low ROX:

Q: How should I store the Thermo Scientific ABsolute QPCR Mix, low ROX?

A: Store the master mix at -20C immediately upon receipt. To maintain stability and performance, avoid repeated freeze-thaw cycles. Always return unused mix promptly to cold storage after each use.

Q: What qPCR instruments are compatible with the low ROX formulation?

A: This mix is compatible with real-time PCR instruments that require or accept low ROX reference dye, allowing broad use across various platforms commonly found in research laboratories.

Q: When should I choose this low ROX qPCR mix over other formulations?

A: Select the low ROX formulation if your qPCR instrument recommends or requires a low level of ROX as a passive reference. This ensures accurate normalization of fluorescence signals and reliable data.

Q: Where can I use the ABsolute QPCR Mix in my research applications?

A: ABsolute QPCR Mix, low ROX, is ideal for a range of applications including gene expression analysis, pathogen detection, and genotyping across DNA and RNA templates in laboratory and research settings.

Q: What are the recommended reaction volumes and setup steps?

A: For optimal results, use reaction volumes between 10-50 L. Simply combine the 2X master mix with your primers, probe (if applicable), and DNA or cDNA template, then proceed with manual pipetting for reaction setup.

Q: How does the mix ensure high sensitivity and accurate quantification?

A: The master mix features a thermostable DNA polymerase, optimized buffer, dNTPs, and proprietary enhancers, ensuring efficient amplification, high specificity, and a dynamic range extending up to 8 logs.

Q: What are the main benefits of using this ready-to-use qPCR master mix?

A: This master mix simplifies your workflow, saves time, and increases consistency between runs, while providing precise, sensitive, and reproducible results for quantitative PCR applications.

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