Product Description
Brand Qiagen
Product Type Extraction Kit
Pack Size 50 T
CAT No 80234
Application Molecular Reagent
For reliable comparison of genomic and transcriptomic data, purification of DNA and RNA from the same sample is essential. The AllPrep DNA/RNA FFPE Kit uses a patent-pending solubilization method to purify DNA and RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified analytes are suitable for use in applications such as real-time PCR and Pyrosequencing. The kit can be automated on theQIAcube Connect.
Simultaneous Extraction of DNA and RNAWith the Qiagen AllPrep DNA/RNA FFPE Kit, you can purify both DNA and RNA from the same FFPE tissue section, streamlining sample processing for molecular biology research workflows. The kit's dual extraction protocol ensures efficient recovery of both nucleic acid types while minimizing sample loss.
Optimized for FFPE SamplesThis kit is specifically tailored for formalin-fixed, paraffin-embedded tissue sections, addressing the challenges of cross-linking and fragmentation. Its robust protocol and included reagents maximize yield and integrity of extracted nucleic acids for reliable research outcomes.
Easy Manual Operation and Short Processing TimeRequiring only standard laboratory equipment-such as a microcentrifuge, heat block, and pipette-each run processes up to ten samples in roughly 85 minutes. The manual, spin column-based workflow is straightforward, minimizing hands-on time and technical barriers.
FAQ's of Qiagen AllPrep DNA/RNA FFPE Kit (50):
Q: How does the Qiagen AllPrep DNA/RNA FFPE Kit work for extracting nucleic acids from FFPE tissue?
A: The kit employs silica-membrane spin column technology to simultaneously isolate high-quality genomic DNA and total RNA from FFPE tissue sections. Samples undergo deparaffinization, lysis, and specific binding conditions to separate DNA and RNA, which are then eluted for further study.
Q: What types of samples and sample amounts can be processed with this kit?
A: It is optimized for formalin-fixed, paraffin-embedded (FFPE) tissue sections, with each sample accommodating up to 10 m-thick sections. This ensures compatibility with common pathology sample formats used in research laboratories.
Q: When should I use this kit in my workflow, and what are the advantages?
A: Use this kit when simultaneous purification of DNA and RNA from a single FFPE sample is necessary, such as for integrated genomics or transcriptomics studies. It offers high yields and saves sample material, enabling comprehensive analyses from limited tissue sections.
Q: Where can the Qiagen AllPrep DNA/RNA FFPE Kit be used?
A: This kit is suitable for research laboratories equipped with basic molecular biology tools. Its portable, manual design allows for use in various settings, requiring only a microcentrifuge, heat block, and pipette.
Q: What is the total processing time, and how many samples can be prepared per kit?
A: Processing time is approximately 85 minutes for 10 samples, and each kit contains sufficient reagents for 50 purifications, allowing efficient, mid-throughput workflows.
Q: How should I store the Qiagen AllPrep DNA/RNA FFPE Kit and what is its shelf life?
A: Store the kit at room temperature (15-25C) as indicated on the label. The typical shelf life ranges from 12 to 24 months, so always refer to the manufacturer's expiration date for optimal performance.
Q: What benefits does simultaneous purification offer for research?
A: Simultaneous DNA and RNA extraction conserves precious FFPE sample material, reduces processing time, and provides parallel datasets from the same specimen, which is valuable for integrative molecular analyses in research settings.