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Qiagen REPLI g Midi Kit (100) Qiagen REPLI g Midi Kit (100) Qiagen REPLI g Midi Kit (100)
Qiagen REPLI g Midi Kit (100)
Qiagen REPLI g Midi Kit (100) Qiagen REPLI g Midi Kit (100)

Qiagen REPLI g Midi Kit (100)

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Product Details:

  • Usage Type Laboratory
  • Shelf Life Refer to manufacturers instructions
  • Instruments Type Kit
  • Storage Instructions Store at 20C
  • Measurement Range Up to 40 g amplified DNA
  • Features High yield, uniform genome amplification, suitable for downstream applications
  • Function Genomic DNA amplification
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Qiagen REPLI g Midi Kit (100) Price And Quantity

Qiagen REPLI g Midi Kit (100) Product Specifications

  • Multiple Displacement Amplification (MDA)
  • DNA Amplification Kit
  • High fidelity amplification
  • Whole genome amplification from small amounts of DNA
  • No
  • Genomic DNA amplification
  • No
  • Plastic, Reagents
  • High yield, uniform genome amplification, suitable for downstream applications
  • Up to 40 g amplified DNA
  • Store at 20C
  • Kit
  • Manual
  • White/Blue (kit components)
  • New
  • Refer to manufacturers instructions
  • Laboratory
  • No

Product Description

Brand Qiagen

Product Type PCR Kit

Pack Size 100T

CAT No 150045

Application Molecular Reagent



The REPLI-g Mini and Midi Kits offer a single tube workflow with higher yields of amplified DNA, while the REPLI-g UltraFast Kit enables highly uniform and accurate whole genome amplification in just 6090 minutes. For high-throughput processing in 96-well format, the streamlined, room-temperature procedure of the REPLI-g Screening Kit enables straightforward liquid handling that requires just 20 minutes. The REPLI-g Screening kit is intended for rapid screening using a range of genotyping, PCR, sequencing or microarray assays.




High Yield Whole Genome Amplification

With the Qiagen REPLI-g Midi Kit, achieve up to 40 g of uniformly amplified DNA from small starting amounts, ensuring ample genetic material for various analyses. This high yield, combined with high accuracy, supports demanding applications including sequencing, array studies, and SNP analysis.


Broad Sample Compatibility

This kit is versatile, supporting whole genome amplification from genomic DNA, whole blood, cultured or sorted cells, and microdissected samples across human, animal, plant, and microbial sources. Its broad compatibility makes it ideal for research laboratories handling diverse specimens.


Ease of Use and Storage

The REPLI-g Midi Kit comes packaged with pre-aliquoted reagents and an easy-to-follow protocol. For optimal performance, store the kit at -20C according to the manufacturer's instructions. The manual, non-portable format is designed for reliable operation in laboratory settings.

FAQ's of Qiagen REPLI g Midi Kit (100):


Q: How do I use the Qiagen REPLI-g Midi Kit for DNA amplification?

A: To use the kit, combine your DNA sample (10 ng to 1 g) with the provided pre-aliquoted reagents per the included protocol. Incubate the reaction at the recommended temperature for 8 hours to achieve whole genome amplification.

Q: What sample types are compatible with this kit?

A: The kit supports genomic DNA from a wide variety of sources, including whole blood, cultured cells, sorted cells, microdissected samples, and is compatible with human, animal, plant, and microbial DNA.

Q: When should I use this kit in my workflow?

A: Use the REPLI-g Midi Kit when you need to generate large amounts of genomic DNA from low-input samples for downstream applications such as sequencing, SNP genotyping, array analysis, or DNA archiving.

Q: Where should I store the Qiagen REPLI-g Midi Kit?

A: Store the kit at -20C as specified in the manufacturer's instructions to maintain reagent integrity and ensure reliable performance.

Q: What is the process and typical yield per reaction?

A: The amplification process requires about 8 hours, and each reaction can yield up to 40 g of high-fidelity, uniformly amplified DNA for downstream analysis.

Q: What are the key benefits of using this DNA amplification kit?

A: The main advantages include high DNA yield, uniform amplification across the genome, compatibility with multiple sample types, and reliable results suited for sensitive applications like genotyping, sequencing, and archiving.

Q: Is the kit suited for real-time or portable operations?

A: No, the REPLI-g Midi Kit is designed for manual use in laboratories. It is not intended for real-time or portable applications.

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