Thermo Fisher Scientific Applied Biosystems Absolute Q Universal DNA Digital PCR Master Mix (5X)

Thermo Fisher Scientific Applied Biosystems Absolute Q Universal DNA Digital PCR Master Mix (5X)

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Product Details:

Features High precision DNA quantification; optimized for digital PCR efficiency and sensitivity; compatible with hydrolysis probe and intercalating dye chemistries
Shelf Life 12 months when stored properly
Accuracy High, suitable for absolute quantification of DNA
Function Facilitates partitioned digital PCR assays
Instruments Type For use with Absolute Q Digital PCR System
Storage Instructions Store at -20C; avoid repeated freezethaw cycles
Usage Type Single-use (consumable reagent)
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Thermo Fisher Scientific Applied Biosystems Absolute Q Universal DNA Digital PCR Master Mix (5X) Price And Quantity

Thermo Fisher Scientific Applied Biosystems Absolute Q Universal DNA Digital PCR Master Mix (5X) Product Specifications

Condition New
Color Clear to off-white (solution)
Measurement Range Wide dynamic range; suited for rare and abundant target detection
Real-Time Operation Yes, compatible with Absolute Q dPCR System
Usage Type Single-use (consumable reagent)
Use Quantification of DNA targets using digital PCR
Storage Instructions Store at -20C; avoid repeated freezethaw cycles
Shelf Life 12 months when stored properly
Accuracy High, suitable for absolute quantification of DNA
Function Facilitates partitioned digital PCR assays
Technology Digital PCR (dPCR)
Features High precision DNA quantification; optimized for digital PCR efficiency and sensitivity; compatible with hydrolysis probe and intercalating dye chemistries
Equipment Type Digital PCR Master Mix
Operating Type Manual/Automated (for use in dPCR workflows)
Instruments Type For use with Absolute Q Digital PCR System
Power Source Not Applicable (reagent only)
Wall Mounted No
Material Proprietary enzyme blend and reaction buffer

Product Description

Brand	Thermo Fisher Scientific
Product Type	PCR Master Mix
Pack Size	200 Reactions
CAT No	A72710
Application	Molecular Biology

The Applied Biosystems Absolute Q Universal DNA dPCR Master Mix (5X) is designed to lower the barrier to entry for digital PCR (dPCR). It provides you with access to more than 20 million predesigned Applied Biosystems TaqMan Assays for use on your Applied Biosystems QuantStudio Absolute Q Digital PCR System and QuantStudio Absolute Q AutoRun dPCR Suite. Hereas how it can help revolutionize your research.

Optimized Digital PCR Performance

This master mix is formulated specifically for the Absolute Q Digital PCR System, ensuring high efficiency during DNA target quantification. Its proprietary enzyme blend and balanced buffer system maximize sensitivity and compatibility with both hydrolysis probes and intercalating dyes, supporting precise and reproducible results even for rare targets.

Flexible DNA Sample Compatibility

Compatible with a variety of DNA types-including genomic DNA (gDNA), complementary DNA (cDNA), or pre-amplified amplicons-this master mix streamlines workflows. Whether quantifying abundant sequences or rare mutations, consistent reaction conditions allow researchers to detect and quantify targets with confidence.

Reliable & Stable Reagent for Advanced Genetics

Storing the master mix at -20C ensures up to twelve months of consistent performance, provided freeze-thaw cycles are minimized. The clear to off-white solution is a ready-to-use consumable, ideal for single-use digital PCR applications and reliable across a wide dynamic range of DNA concentrations.

FAQ's of Thermo Fisher Scientific Applied Biosystems Absolute Q Universal DNA Digital PCR Master Mix (5X):

Q: How should I store the Absolute Q Universal DNA Digital PCR Master Mix (5X) for optimal stability?

A: Store the master mix at -20C and avoid repeated freeze-thaw cycles to maintain product integrity. When stored properly, it remains stable for up to twelve months.

Q: What types of DNA samples are compatible with this master mix?

A: The mix is suitable for genomic DNA (gDNA), cDNA, and DNA amplicons, facilitating diverse applications in molecular genetic analyses.

Q: When is it recommended to use this master mix?

A: Use this product whenever precise absolute quantification, copy number variation analysis, or rare mutation detection is required in research or diagnostics using the Absolute Q Digital PCR System.

Q: Where can I use this digital PCR master mix?

A: This reagent is intended for use exclusively with the Applied Biosystems Absolute Q Digital PCR System and is best utilized in laboratories equipped with this platform.

Q: What is the process for preparing reactions with this master mix?

A: Begin by preparing your DNA sample and detecting chemistry (hydrolysis probe or intercalating dye). Mix the 5X master mix with your template and detection chemistry, and follow the Absolute Q System's protocol for partitioning and digital PCR cycling.

Q: How does this master mix benefit advanced genetic analysis?

A: The proprietary formulation ensures high accuracy, sensitivity, and reproducibility, enabling confident detection and quantification of both abundant and rare DNA targets within digital PCR applications.

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