Thermo Fisher Scientific Applied Biosystems Absolute Q Universal DNA Digital PCR Master Mix (5X)

Thermo Fisher Scientific Applied Biosystems Absolute Q Universal DNA Digital PCR Master Mix (5X)

Get a Price/Quote

Product Details:

Instruments Type For use with Absolute Q Digital PCR System
Storage Instructions Store at -20C; avoid repeated freezethaw cycles
Features High precision DNA quantification; optimized for digital PCR efficiency and sensitivity; compatible with hydrolysis probe and intercalating dye chemistries
Usage Type Single-use (consumable reagent)
Measurement Range Wide dynamic range; suited for rare and abundant target detection
Function Facilitates partitioned digital PCR assays
Shelf Life 12 months when stored properly
Click to View more
Share Your Product:

Request to Call Back Send Inquiry

Thermo Fisher Scientific Applied Biosystems Absolute Q Universal DNA Digital PCR Master Mix (5X) Price And Quantity

Sample Type DNA (including gDNA, cDNA, or amplicons)
Format 5X concentrated master mix
Reaction Volume Optimized for microfluidic partition volume of Absolute Q System
Chemistries Supported Hydrolysis probe (TaqMan), intercalating dye
Enzyme Type Hot-start DNA polymerase
Compatible Platform Applied Biosystems Absolute Q Digital PCR System
Stability Stable for up to twelve months at -20°C
Application Absolute quantification, copy number variation, rare mutation detection, and other advanced molecular genetic analyses
Catalog Number A41537

Thermo Fisher Scientific Applied Biosystems Absolute Q Universal DNA Digital PCR Master Mix (5X) Product Specifications

Real-Time Operation Yes, compatible with Absolute Q dPCR System
Shelf Life 12 months when stored properly
Technology Digital PCR (dPCR)
Accuracy High, suitable for absolute quantification of DNA
Function Facilitates partitioned digital PCR assays
Condition New
Equipment Type Digital PCR Master Mix
Operating Type Manual/Automated (for use in dPCR workflows)
Color Clear to off-white (solution)
Wall Mounted No
Usage Type Single-use (consumable reagent)
Measurement Range Wide dynamic range; suited for rare and abundant target detection
Features High precision DNA quantification; optimized for digital PCR efficiency and sensitivity; compatible with hydrolysis probe and intercalating dye chemistries
Power Source Not Applicable (reagent only)
Use Quantification of DNA targets using digital PCR
Instruments Type For use with Absolute Q Digital PCR System
Storage Instructions Store at -20C; avoid repeated freezethaw cycles
Material Proprietary enzyme blend and reaction buffer

Sample Type DNA (including gDNA, cDNA, or amplicons)
Format 5X concentrated master mix
Reaction Volume Optimized for microfluidic partition volume of Absolute Q System
Chemistries Supported Hydrolysis probe (TaqMan), intercalating dye
Enzyme Type Hot-start DNA polymerase
Compatible Platform Applied Biosystems Absolute Q Digital PCR System
Stability Stable for up to twelve months at -20°C
Application Absolute quantification, copy number variation, rare mutation detection, and other advanced molecular genetic analyses
Catalog Number A41537

Product Description

Brand	Thermo Fisher Scientific
Product Type	PCR Master Mix
Pack Size	200 Reactions
CAT No	A72710
Application	Molecular Biology

The Applied Biosystems Absolute Q Universal DNA dPCR Master Mix (5X) is designed to lower the barrier to entry for digital PCR (dPCR). It provides you with access to more than 20 million predesigned Applied Biosystems TaqMan Assays for use on your Applied Biosystems QuantStudio Absolute Q Digital PCR System and QuantStudio Absolute Q AutoRun dPCR Suite. Hereas how it can help revolutionize your research.

Optimized Digital PCR Performance

This master mix is formulated specifically for the Absolute Q Digital PCR System, ensuring high efficiency during DNA target quantification. Its proprietary enzyme blend and balanced buffer system maximize sensitivity and compatibility with both hydrolysis probes and intercalating dyes, supporting precise and reproducible results even for rare targets.

Flexible DNA Sample Compatibility

Compatible with a variety of DNA types-including genomic DNA (gDNA), complementary DNA (cDNA), or pre-amplified amplicons-this master mix streamlines workflows. Whether quantifying abundant sequences or rare mutations, consistent reaction conditions allow researchers to detect and quantify targets with confidence.

Reliable & Stable Reagent for Advanced Genetics

Storing the master mix at -20C ensures up to twelve months of consistent performance, provided freeze-thaw cycles are minimized. The clear to off-white solution is a ready-to-use consumable, ideal for single-use digital PCR applications and reliable across a wide dynamic range of DNA concentrations.

FAQ's of Thermo Fisher Scientific Applied Biosystems Absolute Q Universal DNA Digital PCR Master Mix (5X):

Q: How should I store the Absolute Q Universal DNA Digital PCR Master Mix (5X) for optimal stability?

A: Store the master mix at -20C and avoid repeated freeze-thaw cycles to maintain product integrity. When stored properly, it remains stable for up to twelve months.

Q: What types of DNA samples are compatible with this master mix?

A: The mix is suitable for genomic DNA (gDNA), cDNA, and DNA amplicons, facilitating diverse applications in molecular genetic analyses.

Q: When is it recommended to use this master mix?

A: Use this product whenever precise absolute quantification, copy number variation analysis, or rare mutation detection is required in research or diagnostics using the Absolute Q Digital PCR System.

Q: Where can I use this digital PCR master mix?

A: This reagent is intended for use exclusively with the Applied Biosystems Absolute Q Digital PCR System and is best utilized in laboratories equipped with this platform.

Q: What is the process for preparing reactions with this master mix?

A: Begin by preparing your DNA sample and detecting chemistry (hydrolysis probe or intercalating dye). Mix the 5X master mix with your template and detection chemistry, and follow the Absolute Q System's protocol for partitioning and digital PCR cycling.

Q: How does this master mix benefit advanced genetic analysis?

A: The proprietary formulation ensures high accuracy, sensitivity, and reproducibility, enabling confident detection and quantification of both abundant and rare DNA targets within digital PCR applications.

Tell us about your requirement

Price:

Quantity

Select Unit

50
100
200
250
500
1000+

Additional detail

Mobile number

Other Products in 'Medical Reagents' category

Cepheid Xpert HIV-1 Viral Load Features : Rapid results, cartridgebased sample processing, minimal handson time, integrated quality control Function : Detection and quantification of HIV1 viral load Real-Time Operation : Yes Usage Type : Laboratory or PointofCare Operating Type : Other, Automatic/Automated Equipment Type : Molecular Diagnostic System Use : Quantitative detection of HIV1 RNA in human plasma Material : Other, Plastic Components, Electronic Parts Storage Instructions : Store cartridges at 228C Instruments Type : Molecular Diagnostic Analyzer Shelf Life : 18 months for cartridges (unopened at recommended storage) Technology : RealTime PCR (Polymerase Chain Reaction) Portable : Yes Wall Mounted : No Color : White with blue accents Condition : New Accuracy : High (meets WHO performance criteria) Measurement Range : 40 to 10,000,000 copies/mL Power Source : Electric Weight : 10 kg (Approx.) Dimension (L*W*H) : 27 cm x 36 cm x 35 cm (Approx.) Display Type : Digital/LED Display Frequency : 5060 Hz Voltage : 100240V AC, 5060Hz Power Consumption : 60 W Noise Level : Low

Send Inquiry

Cepheid Xpert Xpress Flu/RSV Features : Sampletoanswer system, Random access, Automated results interpretation, CLIA waived Function : Multiplex detection of viral RNA Real-Time Operation : Yes Usage Type : Clinical Laboratory, PointofCare Operating Type : Other, Automated Equipment Type : Molecular Diagnostic Instrument Use : Detection of Influenza A, Influenza B, and Respiratory Syncytial Virus (RSV) Material : Other, Highgrade medical plastics and electronic components Storage Instructions : Store cartridges at 228C Instruments Type : Automated Analyzer Shelf Life : Cartridges: up to 12 months (check packaging) Technology : RealTime RTPCR Portable : Yes Wall Mounted : No Color : White / Blue Condition : New Accuracy : 95% Measurement Range : Qualitative detection Power Source : Electric Weight : <7 kg Dimension (L*W*H) : 27.4 x 36.6 x 18.4 cm Display Type : Touchscreen LCD Frequency : 50/60 Hz Voltage : 100240 V AC Power Consumption : Approx. 60 VA Noise Level : Low

Send Inquiry

Qiagen dPCR CGT Assay GFP (FAM) Features : Highly specific GFP gene detection, optimized for cell and gene therapy workflows, high sensitivity Function : Quantitative detection of GFP gene for CGT applications Real-Time Operation : Other, Compatible with realtime digital PCR platforms Usage Type : Laboratory/Research Operating Type : Other, Automated/Manual (depending on platform) Equipment Type : Digital PCR (dPCR) Assay Kit Use : Detection and quantification of GFP (Green Fluorescent Protein) gene Material : Other, Reagent mix for PCR analysis Storage Instructions : Store at 15C to 25C Instruments Type : Digital PCR instruments (for example, QIAcuity dPCR System) Shelf Life : Refer to kit label or datasheet; typically 612 months Technology : dPCR Digital Polymerase Chain Reaction Portable : Yes (kit format) Wall Mounted : No Color : Standard reagent colorless/clear; packaging as per manufacturer Condition : New Accuracy : High accuracy for absolute quantification (dependent on instrument calibration) Measurement Range : Broad dynamic range (platformdependent) Power Source : Other, Not Applicable (Used with compatible dPCR instruments) Weight : ~40100 g (complete kit) Dimension (L*W*H) : Kit packaging dimensions vary

Send Inquiry

Thermo Fisher Scientific Applied Biosystems TaqPath 1-Step Multiplex Master Mix (No ROX) Features : Sensitive and specific multiplex detection, fast RT, thermostable enzymes, dUTP/UNG contamination control, no ROX reference dye Function : Onestep reverse transcription and quantitative PCR in multiplex format Real-Time Operation : Yes Usage Type : Laboratory, Research, Diagnostic Operating Type : Other, Automated, compatible with realtime PCR instruments Equipment Type : Multiplex RealTime PCR Master Mix Use : Multiplex detection of RNA targets in onetube RTqPCR reactions (e.g. pathogen detection, gene expression studies) Material : Other, Proprietary enzyme blend and optimized buffer ingredients (contains dNTPs, thermostable MMLV reverse transcriptase, thermostable DNA polymerase) Storage Instructions : Store at 20C; avoid repeated freezethaw cycles Instruments Type : For use with realtime PCR instruments (without ROX normalization) Shelf Life : 12 months from date of manufacture Technology : 1Step RealTime RTqPCR (Multiplex) Portable : No (requires laboratory equipment) Wall Mounted : No Color : Colorless/clear liquid Condition : New Accuracy : Highly accurate for multiplex quantitative RNA analysis Measurement Range : Wide dynamic range suitable for high and low target copy detection Weight : Not Applicable (reagent format) Dimension (L*W*H) : Not Applicable (supplied in reagent vial/pack)

Send Inquiry

SEND INQUIRY CALL ME FREE