Qiagen dPCR CGT Assay GFP (FAM)

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Product Details:

Storage Instructions Store at -15C to -25C
Accuracy High accuracy for absolute quantification (dependent on instrument calibration)
Instruments Type Digital PCR instruments (for example, QIAcuity dPCR System)
Usage Type Laboratory/Research
Function Quantitative detection of GFP gene for CGT applications
Measurement Range Broad dynamic range (platform-dependent)
Shelf Life Refer to kit label or datasheet; typically 6-12 months
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Qiagen dPCR CGT Assay GFP (FAM) Price And Quantity

Qiagen dPCR CGT Assay GFP (FAM) Product Specifications

Usage Type Laboratory/Research
Function Quantitative detection of GFP gene for CGT applications
Shelf Life Refer to kit label or datasheet; typically 6-12 months
Dimension (L*W*H) Kit packaging dimensions vary
Measurement Range Broad dynamic range (platform-dependent)
Weight ~40-100 g (complete kit)
Features Highly specific GFP gene detection, optimized for cell and gene therapy workflows, high sensitivity
Wall Mounted No
Real-Time Operation Compatible with real-time digital PCR platforms
Portable Yes (kit format)
Use Detection and quantification of GFP (Green Fluorescent Protein) gene
Power Source Not Applicable (Used with compatible dPCR instruments)
Storage Instructions Store at -15C to -25C
Technology dPCR Digital Polymerase Chain Reaction
Operating Type Automated/Manual (depending on platform)
Condition New
Equipment Type Digital PCR (dPCR) Assay Kit
Accuracy High accuracy for absolute quantification (dependent on instrument calibration)
Color Standard reagent colorless/clear; packaging as per manufacturer
Material Reagent mix for PCR analysis
Instruments Type Digital PCR instruments (for example, QIAcuity dPCR System)

Product Description

Brand Qiagen

Product Type PCR and RT-PCR Kit

Pack Size 500 x 12 L

CAT No 250236

Application Molecular Reagent

Adeno-associated virus (AAV) is a widely used viral vector in gene therapy applications. However, the generation and purification of the viral vectors require rigorous quality control to enable safe and reliable dosing during clinical studies or patient care. The ability to accurately quantify vector titers and detect contamination is critical for safe and effective AAV-based gene therapies.

Advanced Digital PCR Technology for Reliable Quantification

Employing digital PCR, the assay delivers absolute quantification of the GFP gene, making it ideal for vector copy number determination and precise quality control in cell and gene therapy workflows. The high sensitivity ensures detection even at low copy numbers, meeting rigorous research and clinical demands.

Convenient All-in-One Kit Format

This assay kit arrives fully equipped with all necessary reagents and a comprehensive protocol, streamlining laboratory workflows. Its compatibility with QIAcuity and other leading dPCR systems offers researchers flexibility and confidence in their platform choice, while ensuring accurate GFP gene measurement.

Optimized for Research and Clinical Applications

Designed for laboratory and research use, the Qiagen dPCR CGT Assay GFP (FAM) supports a broad range of applications, including research studies, clinical sample analysis, and monitoring gene therapy vector integration. Its robust reagent formulation guarantees reproducibility and high data quality across studies.

FAQ's of Qiagen dPCR CGT Assay GFP (FAM):

Q: How does the Qiagen dPCR CGT Assay GFP (FAM) detect the GFP gene?

A: The assay uses a probe-based digital PCR format with FAM fluorescence to specifically detect and quantify the Green Fluorescent Protein (GFP) gene in DNA samples, ensuring high sensitivity and specificity.

Q: What types of samples can be analyzed with this kit?

A: You can use DNA samples from research or clinical sources, making it suitable for applications in both laboratory investigations and cell/gene therapy quality control.

Q: When should I use this assay in my workflow?

A: This assay is ideal for use during quality control steps in gene or cell therapy projects, research studies involving GFP, or when you need accurate vector copy number determination.

Q: Where can I operate this assay?

A: The kit is designed for laboratory settings and is compatible with digital PCR instruments such as the QIAGEN QIAcuity, ensuring flexibility for various research environments.

Q: What is the process for using this kit?

A: Simply follow the included full protocol to prepare your DNA sample, add reagents, and use a compatible dPCR platform for analysis. The workflow is streamlined for both automated and manual digital PCR systems.

Q: How does this kit benefit GFP gene quantification studies?

A: With high sensitivity and accuracy, the assay provides reliable absolute quantification of the GFP gene, even at low copy numbers. This feature is crucial for vector copy number determination and robust quality control in gene therapy applications.

Q: What are the storage requirements and shelf life of the kit?

A: Store the kit at -15C to -25C as instructed on the label or datasheet. The typical shelf life is 6-12 months, ensuring long-term usability for scheduled research projects.

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