Product Description
Brand Qiagen
Product Type PCR Kit
Pack Size 2400T
CAT No 221417
Application Molecular Reagent
The QIAprep& Viral RNA UM Kit is an innovative liquid-based approach to viral RNA epidemiology. The kit is optimized for ultrafast sample preparation and sensitive real-time PCR detection of enveloped RNA viruses such as coronaviruses from human samples (nasal, oro- and nasopharyngeal swabs) collected in non-fixation transport media like UTM, VTM, PBS, 0.9% NaCl, Virocult and eSwab. The kit is also compatible with neat saliva and gargle samples. Fewer and simpler workflow steps accelerate the turnaround time and increase testing frequency while decreasing plastic usage, cost and hands-on time.
Simplified Workflow for High-Throughput Viral RNA ExtractionThe QIAprep& Viral RNA UM Kit streamlines the extraction process, making it suitable for laboratories handling large numbers of samples. Its magnetic bead-based technology ensures consistent, reliable results, while compatibility with automated liquid handling systems enhances productivity. The protocol can also be performed manually, offering flexibility for different laboratory settings.
Reliable Results for Downstream Molecular ApplicationsOptimized for human swab specimens, this kit guarantees high RNA purity and yield, contributing to the accuracy and sensitivity of RT-PCR and other molecular diagnostic assays. The inclusion of UNG decontamination and room temperature shipping further supports the integrity and ease of use of the kit.
Comprehensive Kit Components and Easy StorageEach kit provides all necessary reagents, including lysis buffer, wash buffer, magnetic beads, and elution buffer, along with clear instructions. The kit features a shelf life of 12 months from the manufacturing date and should be stored at 2-8C, ensuring stability and readiness for immediate use in research applications.
FAQ's of Qiagen QIAprep& Viral RNA UM Kit (2400):
Q: How does the QIAprep& Viral RNA UM Kit simplify the RNA extraction process?
A: This kit uses magnetic bead-based technology for efficient isolation and purification of viral RNA. It provides a streamlined workflow requiring minimal hands-on time and supports both automated and manual protocols, accommodating various laboratory setups.
Q: What types of samples are compatible with this viral RNA extraction kit?
A: The kit is designed for use with human swab specimens, specifically nasopharyngeal and oropharyngeal samples, making it suitable for studies involving respiratory viruses.
Q: When should I use this kit in my research workflow?
A: Use this kit when there is a need to isolate and purify viral RNA prior to performing RT-PCR or other molecular diagnostics in a research setting. Its high throughput capacity is ideal for studies processing large numbers of samples quickly.
Q: Where can the Qiagen QIAprep& Viral RNA UM Kit be utilized?
A: This kit is suitable for research laboratories, clinical research centers, and molecular biology facilities in India and other regions, provided it is used strictly for research as it is not approved for diagnostic procedures everywhere.
Q: What is the process of using this kit for RNA extraction?
A: The kit process involves adding lysis buffer to the sample, binding RNA to the magnetic beads, washing away impurities, and finally eluting purified RNA in 20-50 l. The entire protocol can be completed in approximately 30 minutes per run, with detailed instructions provided.
Q: What are the benefits of using this viral RNA extraction kit?
A: Key benefits include high yield and purity of viral RNA, rapid processing time, compatibility with both automated and manual platforms, and stable storage at 2-8C. The kit also allows for room temperature shipment, enhancing convenience.
Q: Is this kit suitable for diagnostic use?
A: The QIAprep& Viral RNA UM Kit is intended for research use only (RUO) and is not approved for diagnostic applications in all regions. It should be used in accordance with local regulations and laboratory standards.