Qiagen RT SYBR Green ROX qPCR Mastermix (24)

Qiagen RT SYBR Green ROX qPCR Mastermix (24)

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Product Details:

Usage Type Laboratory / Research
Instruments Type Compatible with qPCR thermal cyclers supporting ROX
Function Facilitates real-time detection and quantitation of PCR products
Accuracy High specificity and sensitivity for qPCR
Features Contains SYBR Green dye, ROX reference dye, for quantitative detection
Shelf Life 12 months (when stored properly)
Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
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Qiagen RT SYBR Green ROX qPCR Mastermix (24) Price And Quantity

Qiagen RT SYBR Green ROX qPCR Mastermix (24) Product Specifications

Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Material Reagent (liquid chemical mixture)
Portable Yes (small reagent vials)
Use Real-time quantitative PCR (qPCR) detection
Dimension (L*W*H) Not Applicable (reagent vials)
Measurement Range Dependent on qPCR instrument and assay, typically 10^1 to 10^7 copies
Condition New
Color Clear to light yellow (solution)
Real-Time Operation Yes
Shelf Life 12 months (when stored properly)
Wall Mounted No
Features Contains SYBR Green dye, ROX reference dye, for quantitative detection
Accuracy High specificity and sensitivity for qPCR
Technology SYBR Green dye-based qPCR
Equipment Type qPCR Mastermix
Function Facilitates real-time detection and quantitation of PCR products
Operating Type Manual for reaction setup, Automated for instrument
Usage Type Laboratory / Research
Instruments Type Compatible with qPCR thermal cyclers supporting ROX

Product Description

Brand Qiagen

Product Type qPCR Kit

Pack Size 24 x 1.35 ml

CAT No 330523

Application Molecular Reagent

RT2 SYBR Green qPCR Mastermixes are highly suited for real-time PCR applications using SYBR Green, and are required for guaranteed qPCR performance of the RT2 qPCR Primer Assays, RT2 Profiler PCR Arrays, RT2 lncRNA qPCR Assays, and RT2 lncRNA PCR Arrays. This Mastermix should be used in qPCR instruments under standard cycling conditions. RT2 SYBR Green qPCR Mastermixes are also suitable for use with EpiTect ChIP and EpiTect Methyl II qPCR Assays and PCR Arrays.

Superior Performance in Real-Time qPCR

The Qiagen RT2 SYBR Green ROX qPCR Mastermix delivers consistent and accurate results for quantitative PCR, thanks to its optimized buffer system. Its engineered formulation ensures precise amplification, reducing the risk of non-specific products. Ideal for research and diagnostic laboratories, this Mastermix enables reliable detection across a broad dynamic range and is compatible with common qPCR platforms.

User-Friendly and Versatile Application

With its all-in-one liquid composition, the Mastermix simplifies qPCR setup and minimizes experimental variation. It is suitable for gene expression analysis, copy number variation, and pathogen detection, streamlining a wide array of molecular biology studies. The clear, ready-to-use solution ensures convenience and consistency for every reaction.

Long Shelf Life and Easy Storage

This product retains its stability and performance when stored at -20C, with a shelf life of up to 12 months. The vials are compact and portable, making storage and handling straightforward. Proper storage protocol helps avoid repeated freeze-thaw cycles, maintaining reagent integrity for reliable results.

FAQ's of Qiagen RTAA2 SYBR Green ROX qPCR Mastermix (24):

Q: How is the Qiagen RT2 SYBR Green ROX qPCR Mastermix used in gene expression analysis?

A: The Mastermix is used to prepare qPCR reactions for quantitative gene expression analysis. Simply add your template DNA and primers to the provided Mastermix and process the reactions in a compatible real-time PCR thermal cycler. The SYBR Green dye enables detection of DNA amplification in real time.

Q: What equipment is required to use this qPCR Mastermix effectively?

A: The Mastermix requires a qPCR thermal cycler that supports SYBR Green dye detection and ROX passive reference normalization. It is suitable for use with various instruments commonly found in research and diagnostics laboratories.

Q: When should the Mastermix be stored and handled in a specific way?

A: To ensure stability and reliability, the Mastermix should be stored at -20C and protected from repeated freeze-thaw cycles. Always follow the storage instructions indicated on the packaging and only thaw the aliquot needed for immediate use.

Q: Where can the Qiagen RT2 SYBR Green ROX qPCR Mastermix be sourced in India?

A: The product can be obtained through authorized dealers, distributors, exporters, manufacturers, suppliers, traders, and wholesalers across India. Verify catalog numbers and lot details for accurate ordering.

Q: What is the process for setting up reactions with this Mastermix?

A: To set up a reaction, combine the Mastermix with your specific assay components-target DNA and primers-in the recommended volumes. Mix gently, aliquot into qPCR tubes or plates, and load into the instrument. Follow the cycling conditions recommended for your assay.

Q: How does the inclusion of ROX dye benefit qPCR results?

A: ROX serves as a passive reference dye, allowing for normalization of signal variations that may arise from pipetting or instrumentation differences. This leads to more accurate and reproducible results across all reactions.

Q: What advantages does this Mastermix offer over traditional PCR reagents?

A: This Mastermix reduces setup time, minimizes manual error, and provides high specificity and sensitivity. Its optimized composition supports a wide measurement range, enhancing detection reliability and reproducibility in real-time quantitative PCR.

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