Product Description
Brand Waters Corp
Product Type Bulk Media
Pack Size 25 g
CAT No WAT030653
Application Protein
Waters Protein-Pak Epoxy-Activated affinity products are silica-based materials with nominal 40 m particle diameter and nominal 500 pore size. The packing is epoxy-activated for ligand coupling. It is capable of rapid purification of large sample volumes at high flow rates.
Efficient and Versatile Affinity MaterialThe Waters Protein-Pak Bulk Epoxy Activated Affinity Material provides researchers with an efficient matrix for affinity chromatography, enabling multiple applications such as protein purification, enzyme immobilization, and antibody capture. Its epoxy activation supports flexible ligand coupling, making it suitable for varied laboratory workflows.
Robust Matrix for Reliable ResultsComprising crosslinked agarose beads with optimal particle and pore sizes, this material ensures reliable separation performance. Its compatibility with a broad pH range and recommended storage conditions provides long-term stability and consistent results across experiments.
FAQ's of Waters Corp Protein-Pak Bulk Epoxy Activated Affinity Material, 25 g:
Q: How is the Protein-Pak Epoxy Activated Affinity Material used in protein purification?
A: The material is employed in affinity chromatography workflows, where proteins are selectively captured via ligand-coupled epoxy-activated agarose beads. After ligand coupling, protein samples are passed through the resin to isolate and purify target molecules.
Q: What are the recommended storage conditions for this material?
A: Store the Protein-Pak Epoxy Activated Affinity Material at 2C to 8C, suspended in the provided 20% ethanol/water solution, and always keep the bottle tightly capped to maintain product integrity.
Q: When should I perform ligand coupling, and what is the capacity?
A: Ligand coupling should be conducted before exposure to the target sample. The resin typically accommodates 10-20 mg of protein per ml, allowing sufficient binding for most laboratory-scale applications.
Q: Where can this product be used and who supplies it?
A: This product is intended for laboratory use in research and industrial settings across India and beyond, and is available through dealers, distributors, exporters, manufacturers, suppliers, traders, and wholesalers.
Q: What is the benefit of epoxy activation in this affinity material?
A: Epoxy activation enables straightforward, stable ligand coupling under mild conditions, enhancing versatility for capturing proteins, immobilizing enzymes, or binding antibodies efficiently.
Q: What is the shelf life and safety recommendation for handling this resin?
A: When stored unopened under recommended conditions, the shelf life is at least two years. Use suitable laboratory safety precautions when handling, such as gloves and eye protection, to minimize exposure risks.
Q: Is this epoxy activated material compatible with automation systems?
A: This model is designed for manual processing, making it ideal for research labs that require hands-on control in affinity chromatography applications.