Qiagen AllTaq PCR Core Kit (5000 U)

Get a Price/Quote

Product Details:

Usage Type Laboratory
Function Enzymatic amplification of DNA templates
Instruments Type Reagent kit
Shelf Life 12 months from manufacture if stored properly
Storage Instructions Store at 20C
Accuracy Highly accurate amplification, minimal non-specific products
Features High specificity, rapid cycling, robust performance, hot-start capability
Click to View more
Share Your Product:

Request to Call Back Send Inquiry

Qiagen AllTaq PCR Core Kit (5000 U) Price And Quantity

Qiagen AllTaq PCR Core Kit (5000 U) Product Specifications

Instruments Type Reagent kit
Color Colorless or as per reagent
Material Reagent chemicals, buffer solutions, enzyme (AllTaq DNA Polymerase)
Equipment Type PCR Core Kit
Technology PCR (Polymerase Chain Reaction)
Operating Type Manual
Use Amplification of DNA by PCR
Usage Type Laboratory
Function Enzymatic amplification of DNA templates
Real-Time Operation No
Portable Yes
Features High specificity, rapid cycling, robust performance, hot-start capability
Accuracy Highly accurate amplification, minimal non-specific products
Storage Instructions Store at 20C
Wall Mounted No
Condition New
Shelf Life 12 months from manufacture if stored properly

Product Description

Brand Qiagen

Product Type MasterMix

Pack Size 5000 U

CAT No 203127

Application Molecular Reagent

The AllTaq Master Mix Kit and the AllTaq PCR Core Kit provide a convenient format for highly sensitive and specific hot-start PCR using any DNA or cDNA template. Both kits are highly suited for all PCR applications and provide the following features: visual pipetting controls, gel loading and tracking dyes, an ultrafast cycling protocol, extreme room temperature stability during and after reaction setup and a 4x-concentrated master mix format, allowing a higher sample input volume.

Superior PCR Performance for Challenging Templates

AllTaq PCR Core Kit offers exceptional amplification efficiency, particularly for complex or GC-rich DNA templates. Its optimized buffer system and Q-Solution improve yield and accuracy, ensuring reliable results even with difficult samples. This makes the kit an excellent choice for diverse PCR needs in research and diagnostic laboratories.

Hot-Start Technology for High Specificity

The antibody-mediated hot-start feature of the AllTaq DNA Polymerase prevents unwanted activity prior to the initial denaturation step, reducing the formation of primer-dimers and non-specific products. This technology results in clean, specific bands and allows for shorter cycling times, enhancing overall PCR workflow efficiency.

Comprehensive, User-Friendly PCR Solution

The kit provides all critical components required for standard and complex PCR, including enzyme, buffer, and additive solutions. With a shelf life of 12 months (when stored at 20C) and compatibility with multiple DNA templates, this reagent kit is both versatile and convenient for routine use in laboratory settings.

FAQ's of Qiagen AllTaq PCR Core Kit (5000 U):

Q: How do I use the Qiagen AllTaq PCR Core Kit for DNA amplification?

A: To use the AllTaq PCR Core Kit, combine the supplied AllTaq DNA Polymerase, 5x PCR Buffer, Q-Solution, dNTP Mix, primers, template DNA, and nuclease-free water as per the protocol. Adjust the reaction volume (10-50 L) and set up the PCR cycling conditions, taking advantage of the kit's reduced cycling times.

Q: What DNA templates are compatible with this PCR kit?

A: The Qiagen AllTaq PCR Core Kit is optimized for amplifying genomic DNA, cDNA, plasmid DNA, and lambda DNA, making it suitable for a wide range of PCR applications, including complex or GC-rich template amplification.

Q: When should I use Q-Solution included in the kit?

A: Q-Solution is recommended when amplifying challenging templates, such as those with high GC content or complex secondary structures. It modifies the melting behavior of DNA, helping to achieve efficient and specific PCR amplification in these cases.

Q: Where should the Qiagen AllTaq PCR Core Kit be stored to maintain its shelf life?

A: For optimal stability and performance, store the kit components at 20C. This ensures the kit remains effective for up to 12 months from the date of manufacture.

Q: What are the benefits of the hot-start feature in this PCR kit?

A: The antibody-mediated hot-start technology prevents premature enzymatic activity during reaction setup. This increases PCR specificity, reduces non-specific amplification, and enables clean results with minimal background noise.

Q: How does this kit improve PCR speed and accuracy?

A: AllTaq PCR Core Kit's robust enzyme formulation and optimized buffer enable reduced cycling times without compromising accuracy. The hot-start mechanism and Q-Solution further enhance specificity, resulting in high-quality, reproducible PCR products with minimal non-specific bands.

Tell us about your requirement

Price:

Quantity

Select Unit

50
100
200
250
500
1000+

Additional detail

Mobile number

Other Products in 'Medical Reagents' category

Mindray Free Prostate Specific Antigen (CLIA) Equipment Type : Free Prostate Specific Antigen (CLIA) Material : Other, Diagnostic reagents, kit components (specific for Mindray platform) Technology : Chemiluminescence Immunoassay (CLIA) Real-Time Operation : Yes Features : High sensitivity, specific detection, suitable for clinical laboratory use, automated application Wall Mounted : No Shelf Life : 1218 months (check label) Instruments Type : Immunoassay reagent kit Use : Detection of free prostate specific antigen (fPSA) in human serum or plasma Operating Type : Automatic Portable : No (requires compatible analyzer) Function : Quantitative detection of fPSA Usage Type : Clinical laboratory diagnosis Storage Instructions : Store at 28C, avoid freezing Accuracy : High (as per Mindray analyzer data) Condition : New Color : As per reagent pack (typically clear/white bottles) Measurement Range : 0.01100 ng/mL (may vary by analyzer) Dimension (L*W*H) : N/A (kit/consumable packaging) Power Source : Other, N/A (used in compatible immunoassay analyzers)

Send Inquiry

Qiagen QIAseq FastSelect Epidemiology Kit (96) Equipment Type : Epidemiology Library Preparation Kit Material : Other, Molecular Biology Grade Reagents and Enzymes Technology : FastSelect rRNA and Viral RNA Depletion Real-Time Operation : No Features : Ultrafast protocol; Compatible with Illumina platforms; Efficient depletion of rRNA and viral RNA from human, mouse, rat, and other epidemiological samples Wall Mounted : No Shelf Life : 12 months (when stored as instructed) Instruments Type : Nucleic Acid Preparation Kit Use : For Preparing RNASeq Libraries by Depleting Host and Virus rRNA/RNA from Epidemiology Samples Operating Type : Manual Portable : Yes Function : Removes unwanted ribosomal and viral RNA for improved RNASeq analysis Usage Type : Laboratory Research Use Only Storage Instructions : Store at 20C upon receipt Accuracy : High specificity for rRNA and viral RNA depletion Condition : New Color : White/Colorless (Reagents in Vials) Dimension (L*W*H) : Box Size: Approximately 20 x 15 x 8 cm Power Source : Other, Not Required (Enzymatic/Chemical Reagents) Weight : Approx. 0.5 kg

Send Inquiry

Thermo Fisher Scientific Applied Biosystems TaqPath 1-Step Multiplex Master Mix (No ROX) Equipment Type : Multiplex RealTime PCR Master Mix Material : Other, Proprietary enzyme blend and optimized buffer ingredients (contains dNTPs, thermostable MMLV reverse transcriptase, thermostable DNA polymerase) Technology : 1Step RealTime RTqPCR (Multiplex) Real-Time Operation : Yes Features : Sensitive and specific multiplex detection, fast RT, thermostable enzymes, dUTP/UNG contamination control, no ROX reference dye Wall Mounted : No Shelf Life : 12 months from date of manufacture Instruments Type : For use with realtime PCR instruments (without ROX normalization) Use : Multiplex detection of RNA targets in onetube RTqPCR reactions (e.g. pathogen detection, gene expression studies) Operating Type : Other, Automated, compatible with realtime PCR instruments Portable : No (requires laboratory equipment) Function : Onestep reverse transcription and quantitative PCR in multiplex format Usage Type : Laboratory, Research, Diagnostic Storage Instructions : Store at 20C; avoid repeated freezethaw cycles Accuracy : Highly accurate for multiplex quantitative RNA analysis Condition : New Color : Colorless/clear liquid Measurement Range : Wide dynamic range suitable for high and low target copy detection Dimension (L*W*H) : Not Applicable (supplied in reagent vial/pack) Weight : Not Applicable (reagent format)

Send Inquiry

Thermo Fisher Scientific Invitrogen ERCC RNA Spike-In Mix. Equipment Type : RNA SpikeIn Mix Material : Other, Synthetic RNA sequences Technology : External RNA Controls Consortium (ERCC) standards Real-Time Operation : Other, Yes, suitable for realtime qPCR and RNASeq workflows Features : Composed of a set of 92 polyadenylated RNA transcripts, spanning a sixlog concentration range Wall Mounted : No Shelf Life : 6 months at 80C (in aliquots) Instruments Type : Compatible with realtime PCR, microarrays, RNA sequencing instruments Use : RNA spikein controls for normalization and quality control in gene expression experiments Operating Type : Other, Manual pipetting Portable : Yes, in its supplied vial Function : Normalization, performance benchmarking, and process monitoring in transcriptomics Usage Type : For research use only, not for diagnostic procedures Storage Instructions : Store at 80C upon receipt; avoid repeated freezethaw cycles Accuracy : Highly precise with known concentration for absolute quantification Condition : New Color : Colorless (in solution) Measurement Range : Six orders of magnitude (from 1 to 10^6 molecules/l) Dimension (L*W*H) : 1.5 ml tube

Send Inquiry

SEND INQUIRY CALL ME FREE