QIAGEN Plasmid Mega Kit (25)

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Product Details:

Usage Type Laboratory, Molecular Biology Research
Instruments Type Nucleic Acid Purification Kit
Function Isolation and purification of plasmid DNA
Storage Instructions Store at room temperature (1525C)
Features High DNA yield, rapid protocol, high purity DNA suitable for all applications
Measurement Range Up to 5000 g plasmid DNA per preparation
Accuracy High efficiency DNA recovery
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QIAGEN Plasmid Mega Kit (25) Price And Quantity

QIAGEN Plasmid Mega Kit (25) Product Specifications

Features High DNA yield, rapid protocol, high purity DNA suitable for all applications
Equipment Type Plasmid DNA Purification Kit
Measurement Range Up to 5000 g plasmid DNA per preparation
Storage Instructions Store at room temperature (1525C)
Portable Yes
Instruments Type Nucleic Acid Purification Kit
Function Isolation and purification of plasmid DNA
Usage Type Laboratory, Molecular Biology Research
Material Plastic (columns, bottles), paper (manual)
Operating Type Manual
Real-Time Operation No
Condition New
Noise Level Silent operation
Shelf Life 12 months
Use Purification of plasmid DNA from bacterial cultures
Accuracy High efficiency DNA recovery
Technology Silica-membrane technology
Color White/Transparent
Wall Mounted No

Product Description

Brand Qiagen

Product Type QIAfilter

Pack Size 100T

CAT No 12245

Application Molecular Reagent

QIAGEN Plasmid Kits provide gravity-flow, anion-exchange tips for purification of transfection-grade plasmid DNA. Lysate clearing and isopropanol precipitation are achieved by centrifugation.

Efficient and Rapid DNA Purification

The kit's streamlined silica-membrane technology facilitates highly efficient isolation of plasmid DNA. Each preparation takes less than an hour, saving valuable laboratory time. High binding capacity ensures that large yields are recovered, making it ideal for high-throughput molecular biology applications.

Optimized for High Yield and Purity

With the ability to purify up to 5000 g of plasmid DNA from up to 150 ml culture volume, this kit provides high-purity DNA suitable for sensitive downstream workflows including sequencing, cloning, and transfection. The protocol ensures removal of contaminants to support reproducible results.

Convenient and Reliable Research Solution

Supplied with all necessary plastic components and an easy-to-follow manual, the QIAGEN Plasmid Mega Kit is designed for laboratory convenience. Its robust shelf life of 12 months at room temperature and silent, manual operation make it a reliable choice for researchers.

FAQ's of QIAGEN Plasmid Mega Kit (25):

Q: How does the QIAGEN Plasmid Mega Kit achieve high DNA yield and purity?

A: The kit uses advanced silica-membrane technology to selectively bind plasmid DNA while contaminants are washed away, resulting in high recovery (up to 5000 g) of pure DNA suitable for sensitive genetic applications.

Q: What types of samples are compatible with this kit?

A: This kit is specifically designed for the purification of plasmid DNA from bacterial cell cultures, accommodating up to 150 ml of culture per preparation.

Q: When should I use the QIAGEN Plasmid Mega Kit for my molecular biology research?

A: You should use this kit when you require large amounts of high-purity plasmid DNA for downstream applications such as sequencing, cloning, transfection, or restriction digestion.

Q: Where should the kit be stored to maintain optimal performance?

A: Store the QIAGEN Plasmid Mega Kit at room temperature (15-25C) to preserve its reagent stability throughout its 12-month shelf life.

Q: What is the protocol process and how long does each preparation take?

A: Each preparation follows a manual protocol involving cell lysis, DNA binding to the silica membrane, washing, and elution. The entire process is completed in less than one hour.

Q: Does the kit require any special equipment for operation?

A: No special or wall-mounted instruments are required. The kit operates manually and is portable, making it suitable for standard laboratory environments.

Q: What are the main benefits of using this kit in a laboratory setting?

A: Key benefits include rapid processing, high efficiency DNA recovery, compatibility with a variety of downstream applications, and consistent, reliable results for research use.

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