Thermo Fisher Scientific Applied Biosystems SYBR Green Universal Master Mix

Thermo Fisher Scientific Applied Biosystems SYBR Green Universal Master Mix

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Product Details:

Storage Instructions Store at -20C, protect from light
Features High specificity, convenience, room temperature stability, compatible with all Applied Biosystems real-time instruments
Function Enables detection, quantification, and analysis of DNA targets in real time PCR
Shelf Life 12 months from date of manufacture
Measurement Range Wide dynamic range, up to 8 logs of input DNA
Accuracy High precision in quantification of nucleic acids
Instruments Type Compatible with Applied Biosystems and other standard real-time PCR instruments
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Thermo Fisher Scientific Applied Biosystems SYBR Green Universal Master Mix Price And Quantity

Thermo Fisher Scientific Applied Biosystems SYBR Green Universal Master Mix Product Specifications

Dimension (L*W*H) Not Applicable (Supplied in 5 mL or 25 mL bottles)
Measurement Range Wide dynamic range, up to 8 logs of input DNA
Condition New
Accuracy High precision in quantification of nucleic acids
Real-Time Operation Yes
Instruments Type Compatible with Applied Biosystems and other standard real-time PCR instruments
Usage Type For research use only (RUO)
Wall Mounted No
Color Fluorescent green (solution appears colorless to light green)
Operating Type Automatic/manual setup for PCR thermal cyclers
Shelf Life 12 months from date of manufacture
Material Proprietary reagent mix (buffer, dNTPs, Taq DNA polymerase, SYBR Green dye)
Function Enables detection, quantification, and analysis of DNA targets in real time PCR
Technology Real-time PCR (qPCR)
Storage Instructions Store at -20C, protect from light
Equipment Type SYBR Green Universal Master Mix
Features High specificity, convenience, room temperature stability, compatible with all Applied Biosystems real-time instruments
Portable Yes
Use Quantitative PCR amplification and detection of nucleic acids

Dye SYBR Green I
Format 2X Master Mix, ready-to-use
Packaging Available in 5 mL, 25 mL, and 125 mL configurations
Lot Number Provided on the product label
Compatibility Optimized for Applied Biosystems 7500, StepOne, QuantStudio and other qPCR systems
Application Gene expression analysis, microbial quantification, GMO detection, and relative/absolute quantification assays
Enzyme AmpliTaq DNA Polymerase, UP
Volume Per Reaction Typically 1050 L reactions
Passive Reference Dye ROX dye present (for normalization, only in certain SKUs)

Product Description

Brand	Thermo Fisher Scientific
Product Type	Master Mix
Pack Size	10 x 5 mL
CAT No	4312704
Application	Molecular Biology

SYBR Green PCR Master Mix Everything you need for SYBR Green dyeabased PCR amplification and detection in a convenient, single-tube format. Applied Biosystems SYBR Green PCR Master Mix combines SYBR Green I dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

High Precision Real-Time Quantification

Experience exceptional accuracy and broad quantification range with the SYBR Green Universal Master Mix, formulated to deliver reliable and reproducible results across up to 8 logs of DNA input. The inclusion of AmpliTaq DNA Polymerase, UP, enables robust amplification while minimizing non-specific signals, supporting quantitative PCR assays of varying complexity.

Convenient and Flexible PCR Workflow

Save valuable laboratory time with this ready-to-use master mix compatible with manual or automatic sample setup. Its format minimizes pipetting errors, and compatibility with all Applied Biosystems real-time platforms streamlines workflow integration, making it an ideal choice for diverse applications such as gene expression and microbial detection.

Optimized Stability and Compatibility

Engineered for room temperature stability during handling, the master mix must be stored at -20C and protected from light for long-term reliability. Available in multiple packaging options, it offers flexibility for research scale-up or routine testing. ROX dye is included for normalization in designated SKUs, further enhancing experimental consistency.

FAQ's of Thermo Fisher Scientific Applied Biosystems SYBR Green Universal Master Mix:

Q: How should I store the SYBR Green Universal Master Mix to maintain its stability?

A: Store the master mix at -20C and protect it from light to preserve its stability throughout the 12-month shelf life. Avoid repeated freeze-thaw cycles to ensure consistent performance in your qPCR assays.

Q: What are the main applications of the SYBR Green Universal Master Mix?

A: This master mix is designed for gene expression analysis, microbial quantification, GMO detection, and both relative and absolute quantification assays, delivering reliable performance in real-time PCR workflows.

Q: When is it beneficial to use the ROX passive reference dye included in some SKUs?

A: The ROX dye serves as a passive reference for signal normalization, improving data reproducibility. Use it when running qPCR assays on platforms that require normalization, such as certain Applied Biosystems instruments.

Q: Where can the SYBR Green Universal Master Mix be used?

A: This product is suitable for research laboratories using Applied Biosystems real-time PCR instruments like the 7500, StepOne, QuantStudio series, and other compatible qPCR systems.

Q: How does the master mix contribute to high-precision qPCR quantification?

A: The proprietary formulation includes optimized buffer components, dNTPs, AmpliTaq DNA Polymerase, and SYBR Green I dye, ensuring high specificity, accurate quantification, and detection of nucleic acids over a wide dynamic range.

Q: What is the recommended reaction volume per qPCR run?

A: Typical reaction volumes range from 10 to 50 L per well, offering flexibility for various throughput requirements and experimental setups.

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