Thermo Fisher Scientific Applied Biosystems SYBR Green Universal Master Mix

Thermo Fisher Scientific Applied Biosystems SYBR Green Universal Master Mix

Get a Price/Quote

Product Details:

Measurement Range Wide dynamic range, up to 8 logs of input DNA
Function Enables detection, quantification, and analysis of DNA targets in real time PCR
Accuracy High precision in quantification of nucleic acids
Features High specificity, convenience, room temperature stability, compatible with all Applied Biosystems real-time instruments
Instruments Type Compatible with Applied Biosystems and other standard real-time PCR instruments
Shelf Life 12 months from date of manufacture
Storage Instructions Store at -20C, protect from light
Click to View more
Share Your Product:

Request to Call Back Send Inquiry

Thermo Fisher Scientific Applied Biosystems SYBR Green Universal Master Mix Price And Quantity

Thermo Fisher Scientific Applied Biosystems SYBR Green Universal Master Mix Product Specifications

Color Fluorescent green (solution appears colorless to light green)
Real-Time Operation Yes
Material Proprietary reagent mix (buffer, dNTPs, Taq DNA polymerase, SYBR Green dye)
Wall Mounted No
Features High specificity, convenience, room temperature stability, compatible with all Applied Biosystems real-time instruments
Portable Yes
Operating Type Automatic/manual setup for PCR thermal cyclers
Technology Real-time PCR (qPCR)
Function Enables detection, quantification, and analysis of DNA targets in real time PCR
Dimension (L*W*H) Not Applicable (Supplied in 5 mL or 25 mL bottles)
Measurement Range Wide dynamic range, up to 8 logs of input DNA
Use Quantitative PCR amplification and detection of nucleic acids
Accuracy High precision in quantification of nucleic acids
Equipment Type SYBR Green Universal Master Mix
Condition New
Shelf Life 12 months from date of manufacture
Instruments Type Compatible with Applied Biosystems and other standard real-time PCR instruments
Usage Type For research use only (RUO)
Storage Instructions Store at -20C, protect from light

Format 2X Master Mix, ready-to-use
Volume Per Reaction Typically 1050 L reactions
Packaging Available in 5 mL, 25 mL, and 125 mL configurations
Dye SYBR Green I
Compatibility Optimized for Applied Biosystems 7500, StepOne, QuantStudio and other qPCR systems
Passive Reference Dye ROX dye present (for normalization, only in certain SKUs)
Application Gene expression analysis, microbial quantification, GMO detection, and relative/absolute quantification assays
Lot Number Provided on the product label
Enzyme AmpliTaq DNA Polymerase, UP

Product Description

Brand	Thermo Fisher Scientific
Product Type	Master Mix
Pack Size	10 x 5 mL
CAT No	4312704
Application	Molecular Biology

SYBR Green PCR Master Mix Everything you need for SYBR Green dyeabased PCR amplification and detection in a convenient, single-tube format. Applied Biosystems SYBR Green PCR Master Mix combines SYBR Green I dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

High Precision Real-Time Quantification

Experience exceptional accuracy and broad quantification range with the SYBR Green Universal Master Mix, formulated to deliver reliable and reproducible results across up to 8 logs of DNA input. The inclusion of AmpliTaq DNA Polymerase, UP, enables robust amplification while minimizing non-specific signals, supporting quantitative PCR assays of varying complexity.

Convenient and Flexible PCR Workflow

Save valuable laboratory time with this ready-to-use master mix compatible with manual or automatic sample setup. Its format minimizes pipetting errors, and compatibility with all Applied Biosystems real-time platforms streamlines workflow integration, making it an ideal choice for diverse applications such as gene expression and microbial detection.

Optimized Stability and Compatibility

Engineered for room temperature stability during handling, the master mix must be stored at -20C and protected from light for long-term reliability. Available in multiple packaging options, it offers flexibility for research scale-up or routine testing. ROX dye is included for normalization in designated SKUs, further enhancing experimental consistency.

FAQ's of Thermo Fisher Scientific Applied Biosystems SYBR Green Universal Master Mix:

Q: How should I store the SYBR Green Universal Master Mix to maintain its stability?

A: Store the master mix at -20C and protect it from light to preserve its stability throughout the 12-month shelf life. Avoid repeated freeze-thaw cycles to ensure consistent performance in your qPCR assays.

Q: What are the main applications of the SYBR Green Universal Master Mix?

A: This master mix is designed for gene expression analysis, microbial quantification, GMO detection, and both relative and absolute quantification assays, delivering reliable performance in real-time PCR workflows.

Q: When is it beneficial to use the ROX passive reference dye included in some SKUs?

A: The ROX dye serves as a passive reference for signal normalization, improving data reproducibility. Use it when running qPCR assays on platforms that require normalization, such as certain Applied Biosystems instruments.

Q: Where can the SYBR Green Universal Master Mix be used?

A: This product is suitable for research laboratories using Applied Biosystems real-time PCR instruments like the 7500, StepOne, QuantStudio series, and other compatible qPCR systems.

Q: How does the master mix contribute to high-precision qPCR quantification?

A: The proprietary formulation includes optimized buffer components, dNTPs, AmpliTaq DNA Polymerase, and SYBR Green I dye, ensuring high specificity, accurate quantification, and detection of nucleic acids over a wide dynamic range.

Q: What is the recommended reaction volume per qPCR run?

A: Typical reaction volumes range from 10 to 50 L per well, offering flexibility for various throughput requirements and experimental setups.

Tell us about your requirement

Price:

Quantity

Select Unit

50
100
200
250
500
1000+

Additional detail

Mobile number

Other Products in 'Medical Reagents' category

Mindray B-type natriuretic peptide (CLIA) Material : Other, Plastic, reagent chemicals Wall Mounted : No Real-Time Operation : Yes Technology : Chemiluminescence Immunoassay (CLIA) Equipment Type : Diagnostic Reagent Kit Usage Type : Clinical use, laboratory use Instruments Type : Compatible with Mindray CL900i, CL1200i platforms Condition : New Use : For quantitative determination of Btype natriuretic peptide (BNP) in human serum/plasma Operating Type : Automatic Features : Rapid, highsensitivity, reliable results Color : White, Blue Accuracy : 98% Function : Cardiac marker detection Storage Instructions : Store at 28C, avoid sunlight Shelf Life : 12 months Measurement Range : 5 5000 pg/mL Noise Level : Silent operation Portable : Yes Weight : Lightweight kit Dimension (L*W*H) : As per product design Frequency : High throughput compatible Power Source : Electric

Send Inquiry

Erba Mannheim System Pack Path Kit Material : Other, Plastic and Specialized Components Wall Mounted : No Real-Time Operation : Yes Technology : Clinical Chemistry Reagent Kit Equipment Type : System Pack Path Kit Usage Type : Pathology Lab Use Instruments Type : Reagent Kits for Autoanalyzer Condition : New Use : For Automated Pathological Testing Operating Type : Other, Automated Features : High Sensitivity, Batch Consistency, Automation Ready Color : White Accuracy : High, As Per Manufacturer Specification Function : Clinical Pathology Analysis Storage Instructions : Store at 28C, Avoid Direct Sunlight Shelf Life : 1218 Months Measurement Range : Defined By Test Protocol Noise Level : Silent Operation Portable : Yes Weight : Approx. 12 kg (Varies by Configuration) Dimension (L*W*H) : Standard Kit Size Frequency : Ready for Daily Lab Use Power Source : Other, Not Required (Reagent Kit)

Send Inquiry

BioRad iScript Reverse Transcription Supermix, 100 x 20 l rxns, 400 l Material : Other, Liquid enzyme formulation Wall Mounted : No Real-Time Operation : Other, Compatible with realtime PCR Technology : cDNA synthesis via reverse transcription Equipment Type : Reverse Transcription Supermix Kit Usage Type : Single use or multiple use under sterile conditions Instruments Type : Reagents for molecular biology Condition : New Use : Synthesis of complementary DNA (cDNA) from RNA templates Operating Type : Other, Manual operation for in vitro laboratory use Features : Readytouse master mix; optimized for high efficiency; minimizes pipetting steps; RNase inhibitor included Color : Clear or pale yellow liquid Accuracy : High specificity and sensitivity for cDNA synthesis Function : Reverse transcription of RNA to cDNA Storage Instructions : Store at 20C; avoid repeated freezethaw cycles Shelf Life : 1 year (when stored at 20C) Measurement Range : Supports RNA input: 1 pg1 g per reaction Portable : Yes (reagent format) Weight : 400 l total reagent volume Dimension (L*W*H) : Not Applicable (Reagent vial)

Send Inquiry

QIAGEN Plasmid Plus Maxi Kit (100) Material : Other, Highquality plastic components, prefilled buffer tubes Wall Mounted : No Real-Time Operation : No Technology : Anionexchange silica membrane technology Equipment Type : Plasmid DNA Purification Kit Usage Type : Laboratory Use Instruments Type : Life Sciences Research Kit Condition : New Use : Isolation and purification of plasmid DNA from bacterial cultures Operating Type : Manual Features : Rapid purification, high yield, high purity, scalable Color : White/Blue Accuracy : High quality DNA yield (A260/A280 ratio: 1.801.90) Function : Plasmid DNA extraction and purification Storage Instructions : Store at room temperature (1525C) Shelf Life : 12 months Measurement Range : For 150750 ml E. coli culture per prep Portable : Yes

Send Inquiry

SEND INQUIRY CALL ME FREE