Product Description
| Brand |
Thermo Fisher Scientific |
| Product Type |
PCR Amplification Kit |
| Pack Size |
200T |
| CAT No |
4476135 |
| Application |
Molecular Biology |
The GlobalFiler PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support.
Advanced Multiplex STR AnalysisThe GlobalFiler PCR Amplification Kit delivers comprehensive STR analysis in a single reaction, covering 24 loci-including gender discrimination and quality controls-to ensure robust identification. Its compatibility with major Genetic Analyzers and sensitive DNA input requirements help streamline forensic workflows. Laboratories benefit from a reliable, standardized solution for both casework and database applications.
Versatile Format and UsageAvailable in both lyophilized and liquid formats, the GlobalFiler kit supports flexible storage and use. Kit sizes accommodate different laboratory needs, and the reagents arrive ready to use with minimal preparation. Designed for both reference and complex casework, this kit is widely utilized by forensic labs, suppliers, and distributors across India.
FAQ's of Thermo Fisher Scientific Applied Biosystems GlobalFiler PCR Amplification Kit:
Q: How does the GlobalFiler PCR Amplification Kit support forensic DNA analysis?
A: The kit streamlines forensic DNA analysis by multiplexing 24 loci, including 21 autosomal STRs, one Y-STR, Amelogenin for gender determination, and two quality control markers. This high-throughput capability enhances discrimination and accuracy in human identification.
Q: What equipment is compatible with the GlobalFiler kit?
A: The kit is validated for use with Applied Biosystems 3500/3500xL, 3730/3730xl, and 3130 series Genetic Analyzers, ensuring seamless integration in labs already using these platforms. It also works with GeneMapper ID-X Software for efficient data analysis.
Q: When and where should the GlobalFiler kit be stored?
A: For optimal stability, store the kit at temperatures between -15C and -25C immediately upon arrival. It ships on dry ice, ensuring product integrity during transit, and should be stored in appropriate cold storage facilities within your laboratory.
Q: What is the process for setting up a PCR reaction with the GlobalFiler kit?
A: Reaction setup is straightforward and can be performed at room temperature or on ice. Combine the included Master Mix with your DNA samples and run the cycling protocol, which takes about 60-90 minutes. The kit is sensitive enough to detect DNA inputs as low as 0.1 ng.
Q: Who can benefit from using the GlobalFiler PCR Amplification Kit?
A: Forensic scientists, human identification specialists, and laboratory technicians in criminal justice and population genetics benefit from the kit's high discrimination power and proven reliability. Suppliers, manufacturers, and distributors across India also find it ideal for serving diverse forensic needs.