Product Description
Brand Qiagen
Product Type PCR Kits
Pack Size 1000 mL
CAT No 159992
Application Molecular Reagent
Generation DNA Purif. Solution and Generation DNA Elution Solution are used with Generation Capture Kits for purification of genomic DNA from whole blood, bone marrow, buffy coat, body fluids, cultured cells, cell suspensions and Gram-negative bacteria. The kit employs a flexible solid-phase system with a unique trifunctional purification matrix and reagents for lysing cells and capturing and releasing DNA. This method does not require prelysing of the sample or precipitation of the DNA.
Efficient DNA Extraction and PurificationThe Qiagen Generation DNA Purification Solution simplifies the process of extracting genomic DNA from varied biological samples. Its advanced formulation enables rapid lysis and purification, yielding high-quality DNA suitable for downstream applications. Compatible with multiple automation platforms, this buffer offers researchers unparalleled flexibility and convenience.
Ready-to-Use and ReliableThis solution comes pre-formulated and ready-to-use, reducing time spent on preparation and minimizing variability. The clear, colorless buffer is stable for up to 12 months when stored at room temperature (15-25C), offering consistent performance and reliable results for your genomic DNA extraction needs.
FAQ's of Qiagen Generation DNA Purif. Solution (1000 ml):
Q: How do I use the Qiagen Generation DNA Purification Solution for DNA extraction?
A: To use the solution, follow the protocol specific to your extraction platform (spin column, vacuum manifold, or robotic automation). Add the appropriate amount to your sample for cell lysis and proceed with the recommended purification steps. Consult the product manual for detailed instructions.
Q: What samples can be processed with this purification solution?
A: The solution is suitable for extracting genomic DNA from animal tissues, cultured cells, and bacterial cells, making it versatile for various laboratory research applications.
Q: When should the DNA Purification Solution be replaced or discarded?
A: It should be used within 12 months from the date of purchase. If any changes in appearance, performance, or contamination are detected, discontinue use and replace the bottle.
Q: Where should I store the DNA Purification Solution for optimal performance?
A: Store the bottle at room temperature, specifically between 15 and 25C, to maintain stability and efficacy for the full shelf life.
Q: What are the process steps involved in genomic DNA extraction using this buffer?
A: The typical workflow includes lysis of the biological sample, binding of DNA to a silica membrane, washing to remove contaminants, and elution of purified DNA. The buffer is central to the lysis and purification steps.
Q: What benefits does the Qiagen Generation DNA Purification Solution provide over other methods?
A: Benefits include high DNA yield, exceptional purity, compatibility with various platforms, fast protocol execution, and the convenience of a ready-to-use formulation, streamlining laboratory workflows.