Qiagen Ni-NTA Agarose (500 ml)

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Product Details:

Function Binds polyhistidine-tagged proteins for purification
Usage Type Laboratory reagent
Storage Instructions Store at 28C, do not freeze
Shelf Life At least 2 years from date of manufacture under recommended storage
Instruments Type Laboratory reagent
Accuracy High specificity for His-tagged proteins
Features High binding capacity, high specificity, reusable under proper conditions, compatible with batch, gravity flow, or column purification methods
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Qiagen Ni-NTA Agarose (500 ml) Price And Quantity

Qiagen Ni-NTA Agarose (500 ml) Product Specifications

Material Agarose beads, Ni-NTA (Nickel-nitrilotriacetic acid) ligand
Accuracy High specificity for His-tagged proteins
Condition New
Features High binding capacity, high specificity, reusable under proper conditions, compatible with batch, gravity flow, or column purification methods
Color Opaque white to off-white suspension
Instruments Type Laboratory reagent
Shelf Life At least 2 years from date of manufacture under recommended storage
Operating Type Manual
Weight Approximately 700 g for 500 ml suspension
Real-Time Operation No
Technology Affinity chromatography
Wall Mounted No
Use Isolation and purification of histidine-tagged proteins
Function Binds polyhistidine-tagged proteins for purification
Storage Instructions Store at 28C, do not freeze
Portable Yes
Usage Type Laboratory reagent
Equipment Type Agarose resin for protein purification

Chemical Compatibility Compatible with common buffers and additives
Application Areas Protein engineering, structural and functional proteomics, recombinant protein production
pH Stability Stable from pH 2.014.0
Matrix 6% cross-linked agarose
Binding Capacity Approx. 2040 mg 6xHis-tagged protein per ml resin
Regeneration Can be regenerated and reused multiple times with appropriate cleaning
Packing Size 500 ml bottle
Form Suspension in 20% ethanol
Bead Diameter ~45165 m

Product Description

Brand Qiagen

Product Type PCR Kits

Pack Size 500 mL

CAT No 30250

Application Molecular Reagent

Ni-NTA Agarose is an affinity chromatography matrix for purifying recombinant proteins carrying a His tag. Histidine residues in the His tag bind to the vacant positions in the coordination sphere of the immobilized nickel ions with high specificity and affinity. Cleared cell lysates are loaded onto the matrices. His-tagged proteins are bound, and other proteins pass through the matrix. After washing, His-tagged proteins are eluted in buffer under native or denaturing conditions.

Efficient Purification of His-Tagged Proteins

With its high binding capacity and specificity, Qiagen Ni-NTA Agarose enables researchers to achieve effective isolation and purification of polyhistidine-tagged proteins. Its robust chemical compatibility and stability across a wide pH range make it ideal for various downstream applications in protein engineering and proteomics studies.

Versatile and Reusable Agarose Resin

This resin can be regenerated and reused multiple times with the proper cleaning regimen, offering cost-effectiveness and convenience. Its form as a suspension in 20% ethanol ensures ease of handling and reduces contamination risks. Suitable for batch, gravity flow, or column purification, it caters to diverse laboratory workflows.

FAQ's of Qiagen Ni-NTA Agarose (500 ml):

Q: How should I store Qiagen Ni-NTA Agarose to maintain its stability?

A: It is recommended to store the Ni-NTA Agarose at 2-8C and never freeze it. Proper storage under these conditions maintains its performance and extends its shelf life to at least two years from the date of manufacture.

Q: What is the process for purifying His-tagged proteins with this agarose resin?

A: Purification involves equilibrating the resin with an appropriate buffer, loading the protein sample, washing away non-specifically bound proteins, and then eluting the His-tagged proteins using imidazole-containing buffer. The resin is compatible with batch, gravity flow, or column methods.

Q: When should I regenerate the Ni-NTA Agarose, and what does that entail?

A: Regeneration is recommended after each use or when a decline in binding capacity is observed. It involves washing with specific cleaning solutions (commonly mild acid/base washes, followed by water and buffer rinses) to remove residual proteins and restore resin activity.

Q: Where can I use this resin within laboratory workflows?

A: Qiagen Ni-NTA Agarose is ideal for academic, industrial, and biotechnology laboratories involved in recombinant protein expression, functional or structural proteomics, and protein engineering. It serves as a laboratory reagent compatible with standard protein purification setups.

Q: What are the main benefits of using this agarose resin for protein purification?

A: Key benefits include high specificity for His-tagged targets, substantial binding capacity, broad chemical and pH compatibility, and the potential for multiple reuses, enabling reliable protein purification with cost and time efficiency.

Q: Can I use Ni-NTA Agarose with different purification equipment types?

A: Yes, this resin is compatible with manual batch procedures, gravity flow devices, or chromatographic columns, enhancing flexibility for various application needs.

Q: What additives or buffers are compatible with Qiagen Ni-NTA Agarose?

A: The resin is chemically compatible with a wide range of common laboratory additives and buffers, allowing customization of purification protocols to the characteristics of your target protein.

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