Product Description
Brand Qiagen
Product Type DNA Isolation Kits
Pack Size 50 Nos
CAT No 56304
Application Molecular Reagent
QIAamp DNA Kits provide silica-membrane-based nucleic acid purification from tissues, swabs, CSF, blood, body fluids or washed cells from urine. In addition, genomic and mitochondrial DNA can be purified from small amounts of fresh or frozen blood, tissue and dried blood spots. Mechanical homogenization is not required as the tissues are lysed enzymatically. DNA purification from 112 samples can be automated on the QIAcube Connect using the dedicated QIAamp DNA Mini QIAcube Kit. Purification of DNA using the QIAamp DNA Micro Kit and QIAamp DNA Mini Kit is also automatable on theQIAcube Connect.
High-Efficiency DNA Extraction for Low-Input SamplesThis kit is engineered to deliver accurate, high-purity DNA even from minimal or degraded samples. Its proprietary silica-membrane technology ensures optimal recovery, making it highly efficient for labs handling low-volume or precious samples, such as forensic specimens or dried blood spots.
Versatile Sample Compatibility and Compact DesignThe QIAamp DNA Micro Kit adapts seamlessly to various sample types, including blood, body fluids, tissue, and swabs, supporting diverse research needs. Its compact kit format, with pre-packaged reagents and columns, is space-saving and portable, suitable for laboratories of any size.
Quick and Reliable Genomic DNA PurificationWith a processing time of less than one hour, this kit streamlines laboratory workflows without sacrificing quality. The easy manual spin-column protocol includes all required buffer solutions and Proteinase K, ensuring consistent results with minimal hands-on time.
FAQ's of Qiagen QIAamp DNA Micro Kit (50):
Q: How can the QIAamp DNA Micro Kit be used for DNA extraction?
A: This kit utilizes a manual spin column protocol based on silica-membrane technology. Simply add your sample (up to 500 l), treat it with the provided buffers and Proteinase K, and perform the recommended spin steps. The purified DNA is then eluted in 20-100 l for downstream applications.
Q: What types of samples are compatible with this DNA extraction kit?
A: The QIAamp DNA Micro Kit is suitable for blood, body fluids, cultured cells, tissue samples, swabs, dried blood spots, and forensic specimens, offering flexibility for a diverse range of research applications.
Q: When is this kit typically used in the laboratory workflow?
A: This kit is employed whenever there is a need to purify high-quality genomic DNA for applications such as PCR, genotyping, and sequencing, particularly when working with limited or challenging samples.
Q: Where should I store the QIAamp DNA Micro Kit for optimal shelf life?
A: Store the kit at room temperature (15-25C) as instructed to maintain its effectiveness for up to 12 months from the date of manufacture.
Q: What is the process and total time required for DNA isolation using this kit?
A: The entire DNA purification process can be completed in less than one hour, thanks to the simple manual protocol, which includes sample lysis, binding, washing, and elution steps.
Q: How does this kit benefit researchers working with low DNA input samples?
A: Its optimized protocol and high-affinity silica-membrane columns ensure efficient recovery of pure DNA, even from very low-input samples, providing reliable results for sensitive downstream analyses.
Q: What are the intended research applications for the isolated DNA?
A: The purified DNA is ideal for a range of molecular biology applications, including PCR, genotyping, sequencing, and other enzymatic reactions, supporting advanced genetic analysis and research.