Thermo Fisher Scientific Applied Biosystems GlobalFiler Express PCR Amplification Kit

Thermo Fisher Scientific Applied Biosystems GlobalFiler Express PCR Amplification Kit

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Product Details:

Storage Instructions Store at 28C (refrigerated)
Usage Type Single-use, laboratory
Features Rapid amplification, 24-locus multiplex, direct amplification from blood, buccal, and other samples
Shelf Life Up to 12 months (when stored as recommended)
Measurement Range Suitable for low to high template DNA amounts
Instruments Type Compatible with Applied Biosystems Genetic Analyzers
Accuracy High, suitable for legal and forensic applications
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Thermo Fisher Scientific Applied Biosystems GlobalFiler Express PCR Amplification Kit Price And Quantity

Thermo Fisher Scientific Applied Biosystems GlobalFiler Express PCR Amplification Kit Product Specifications

Accuracy High, suitable for legal and forensic applications
Features Rapid amplification, 24-locus multiplex, direct amplification from blood, buccal, and other samples
Measurement Range Suitable for low to high template DNA amounts
Instruments Type Compatible with Applied Biosystems Genetic Analyzers
Usage Type Single-use, laboratory
Portable Yes, kit form
Dimension (L*W*H) Kit size varies (packaged for laboratory use)
Storage Instructions Store at 28C (refrigerated)
Use Forensic DNA analysis and human identification
Wall Mounted No
Color Clear/Colorless reagents
Operating Type Automatic (requires compatible thermal cycler)
Shelf Life Up to 12 months (when stored as recommended)
Function Amplification and detection of 24 STR loci for human identification
Real-Time Operation No
Condition New
Weight Kit weight varies
Material PCR grade reagents, plastics, enzymes, and oligonucleotides
Technology Multiplex PCR, STR (Short Tandem Repeat) Analysis
Equipment Type PCR Amplification Kit

Fluorescent Dye Channels Multiple, 6-dye chemistry
Cycling Time As little as 40 minutes
Sample Types Direct amplification from FTA cards, blood, buccal swabs, purified DNA
Enzyme Type Hot-start DNA polymerase
Template Input Direct or up to 2 ng DNA
Kit Formats Available 100, 500, 2000 reaction sizes
Regulatory Status For Research, Forensic and Human Identification Use
Number of Loci Amplified 24
Quality Control Supplied with positive control DNA

Product Description

Brand	Thermo Fisher Scientific
Product Type	PCR Amplification Kit
Pack Size	1000 Reactions
CAT No	4474665
Application	Molecular Biology

The GlobalFiler Express PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler Express kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples.

Seamless STR Profiling for Forensic Applications

Empowering forensic and human identification workflows, the GlobalFiler Express PCR Amplification Kit simplifies the detection of 24 STR loci in one rapid run. Its validated performance allows analysts to process a variety of sample types directly-reducing handling errors and turnaround times. Ideal for crime laboratories and legal settings, the kit streamlines casework and reference sample processing, thanks to its compatibility with Applied Biosystems Genetic Analyzers.

Unmatched Speed and Flexibility

Accelerate your STR analysis without compromising data quality. With a cycling time as short as 40 minutes and minimal sample preparation, the GlobalFiler Express Kit supports low-to-high DNA template amounts directly from FTA cards, blood, buccal swabs, or purified DNA. Its specialized hot-start DNA polymerase ensures precise amplification, while the multiple dye channels enable efficient multiplexing.

FAQ's of Thermo Fisher Scientific Applied Biosystems GlobalFiler Express PCR Amplification Kit:

Q: How does the GlobalFiler Express PCR Amplification Kit streamline forensic DNA analysis?

A: The kit enables simultaneous amplification of 24 STR loci in a single, rapid workflow, allowing direct processing of FTA cards, blood, buccal swabs, and purified DNA. This significantly reduces sample preparation time and boosts throughput in forensic and identification laboratories.

Q: What sample types are compatible with the kit, and do they require purification?

A: The kit directly accepts FTA cards, whole blood, buccal swabs, and purified DNA, eliminating the need for extensive pre-processing or purification for most sample types, thus saving valuable laboratory time.

Q: When should I use each available kit size (100, 500, or 2000 reactions)?

A: Choose the 100-reaction kit for small casework or validation studies, the 500-reaction kit for routine lab workflows, and the 2000-reaction kit for high-throughput applications such as databasing or large-scale projects.

Q: Where should the GlobalFiler Express kit be stored, and what is its shelf life?

A: The kit should be stored refrigerated at 2-8C. When stored under these conditions, it maintains optimal performance for up to 12 months from the date of manufacture.

Q: What is the process for using this kit with genetic analyzers?

A: Following sample addition and PCR setup, amplification is performed in a compatible thermal cycler. The resultant products are then loaded onto Applied Biosystems Genetic Analyzers for automated detection, analysis, and interpretation of STR profiles.

Q: What are the major benefits of using this kit for human identification or forensic work?

A: Key benefits include high accuracy suitable for legal settings, rapid cycling, broad sample compatibility, strong quality control via supplied positive control DNA, and support for a wide range of DNA inputs, ensuring reliable results in time-sensitive investigations.

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