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Thermo Fisher Scientific Applied Biosystems AmpFLSTR Identifiler Plus PCR Amplification Kit
Product Details:
- Measurement Range 16 loci + Amelogenin (gender) marker
- Function Amplification of STR regions for DNA profiling
- Shelf Life 12 months at -15C to -25C
- Instruments Type PCR and Capillary Electrophoresis Compatible
- Features Cofiler Plus Loci Coverage, Enhanced Sensitivity, 5-dye Chemistry, Ready-to-Use Mix, Comprehensive Protocols
- Usage Type Laboratory
- Storage Instructions Store at -15C to -25C; Stable at 2C to 8C for up to 7 days
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Thermo Fisher Scientific Applied Biosystems AmpFLSTR Identifiler Plus PCR Amplification Kit Price And Quantity
Thermo Fisher Scientific Applied Biosystems AmpFLSTR Identifiler Plus PCR Amplification Kit Product Specifications
- Single-use per reaction
- Clear/Colorless reagents with labeled containers
- Store at -15C to -25C; Stable at 2C to 8C for up to 7 days
- Depends on kit size (approx. <1 kg for 200 reactions kit)
- Laboratory
- No
- Cofiler Plus Loci Coverage, Enhanced Sensitivity, 5-dye Chemistry, Ready-to-Use Mix, Comprehensive Protocols
- PCR Amplification Kit
- DNA Profiling for Human Identification
- High, validated for forensic use
- New
- Yes (Laboratory Use)
- No
- Amplification of STR regions for DNA profiling
- Not Required (Reagents Only)
- 16 loci + Amelogenin (gender) marker
- PCR and Capillary Electrophoresis Compatible
- Manual
- Kit/Boxed Packaging
- STR (Short Tandem Repeat) Analysis using Multiplex PCR
- PCR-Grade Reagents in Stabilized Liquid Format
- 12 months at -15C to -25C
Product Description
| Brand | Thermo Fisher Scientific |
| Product Type | PCR Amplification Kit |
| Pack Size | 200T |
| CAT No | 4427368 |
| Application | Molecular Biology |
AmpFLSTR Identifiler Plus PCR Amplification Kit enables forensic analysts to recover more interpretable results from a wider range of DNA evidence samples with increased confidence. The new kit includes the same primers and allelic ladder as the widely used Identifiler Kit and harnesses next-generation PCR amplification technology to help provide enhanced sensitivity, a cleaner baseline, improved performance on mixtures, and the ability to overcome high levels of PCR inhibition.
Comprehensive STR Analysis for Human Identification
AmpFLSTR Identifiler Plus enables robust amplification of 15 STR loci as well as Amelogenin for gender determination, providing high discrimination power suitable for forensic, parentage, and DNA database applications. Through its multiplex format and validated chemistry, the kit ensures consistent, accurate results essential for human identification.
Enhanced Sensitivity and Ready-to-Use Components
Engineered with PCR-grade reagents in a stabilized liquid format, the kit offers ease of preparation with its ready-to-use mixes and clear, labeled containers. The optimized protocols allow for high sensitivity-reliable amplification from minimal DNA input-making it a proven solution where sample quantity may be limited.
Seamless Integration with Leading Genetic Analyzers
Designed for compatibility with Thermo Fisher's ABI PRISM and Applied Biosystems 310, 3130, 3500, and 3730 Genetic Analyzers, the kit ensures streamlined workflow integration. Laboratories benefit from reliable genotyping supported by comprehensive instructions and robust allelic ladders for accurate analysis.
FAQ's of Thermo Fisher Scientific Applied Biosystems AmpFLSTR Identifiler Plus PCR Amplification Kit:
Q: How is the AmpFLSTR Identifiler Plus PCR Amplification Kit used in forensic laboratories?
A: The kit is utilized for amplification of 15 STR loci and the Amelogenin gender marker from human DNA samples. Its validated protocols guide users through DNA profiling for forensic casework, parentage, and relationship testing, supporting applications like criminal investigations and database sample processing.Q: What instruments are recommended for use with this PCR amplification kit?
A: The kit is compatible with ABI PRISM 310, 3130, 3500, and 3730 Genetic Analyzers, which perform PCR product analysis via capillary electrophoresis. These instruments enable efficient separation and detection of the amplified STR fragments for subsequent genotyping.Q: When should input DNA quantity be considered for reliable results?
A: Reliable amplification can be achieved with as little as 100 pg of input DNA per reaction. This allows for sensitive detection even when sample quantities are limited, though optimal results are obtained by following the recommended protocols provided in the user manual.Q: Where should the kit be stored to maintain reagent integrity?
A: Store the kit at temperatures between -15C and -25C to maintain component stability for up to 12 months. For short-term storage (up to 7 days), the kit can be kept at 2C to 8C. Always consult the storage instructions included with the kit for best practice.Q: What is the process for performing STR analysis with this kit?
A: Users prepare the PCR reaction by mixing sample DNA with the provided primer and reaction mixes. After thermal cycling, the amplified products are loaded onto a compatible genetic analyzer, where the allelic ladder helps calibrate fragment sizing for accurate STR genotyping.Q: How does the kit benefit laboratories conducting DNA profiling?
A: Its validated chemistry, high sensitivity, and comprehensive protocols enable reliable and reproducible DNA profiles. This supports laboratories in meeting regulatory standards for forensic and relationship analyses, while optimizing workflow with streamlined reagent preparation and instrument compatibility.Q: What are the advantages of the 5-dye system utilized in this kit?
A: The 5-dye system (6FAM, VIC, NED, PET, LIZ) allows simultaneous detection of multiple loci within a single PCR reaction, increasing throughput and resolution. This multiplexing reduces labor and processing time while maximizing the amount of information obtained per sample.Tell us about your requirement
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