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Invitrogen SuperScript IV One-Step RT-PCR System with Platinum Taq DNA Polymerase
Product Details:
- Usage Type Research and diagnostic laboratories
- Function Reverse transcription and PCR amplification in a single tube
- Instruments Type Compatible with most thermal cyclers
- Accuracy High accuracy for gene expression analysis
- Features High sensitivity, fast and robust performance, inhibitor resistance
- Shelf Life 6 months at -20C
- Storage Instructions Store at -20C; avoid freeze-thaw cycles
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Invitrogen SuperScript IV One-Step RT-PCR System with Platinum Taq DNA Polymerase Price And Quantity
Invitrogen SuperScript IV One-Step RT-PCR System with Platinum Taq DNA Polymerase Product Specifications
- RT-PCR System Kit
- Reagent mix, enzyme, buffer solution
- Manual protocol
- Compatible with most thermal cyclers
- Synthesis of cDNA and PCR amplification from RNA
- Research and diagnostic laboratories
- Reverse transcription and PCR amplification in a single tube
- High accuracy for gene expression analysis
- Not required (laboratory reagent kit)
- Varies by kit size (e.g., 100 reactions)
- Compatible with real-time RT-PCR instruments
- Silent
- 6 months at -20C
- High sensitivity, fast and robust performance, inhibitor resistance
- No
- Colorless to pale yellow solutions
- Detects as little as 10 copies of target RNA
- Not applicable (kit form)
- One-Step RT-PCR with Platinum Taq DNA Polymerase
- New
- Yes
- Store at -20C; avoid freeze-thaw cycles
Product Description
| Brand | Thermo Fisher Scientific |
| Product Type | PCR Amplified cDNA |
| Pack Size | 100T |
| CAT No | 12595100 |
| Application | Molecular Biology |
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance. With this convenient one-step formulation plus gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube.
Efficient Single-Tube RT-PCR Solution
Experience streamlined workflow with our One-Step RT-PCR system, combining reverse transcription and PCR amplification in a single tube. This minimizes sample handling, simplifies setup, and reduces the risk of contamination, enabling high-throughput and reliable results whether you are conducting gene expression studies or pathogen detection.
Broad Compatibility and Flexibility
This system works seamlessly with various RNA templates-including total RNA, poly(A) mRNA, and even challenging viral or bacterial RNA. Compatible with gene-specific primers, random hexamers, or oligo(dT), the kit supports diverse experimental needs in both basic research and diagnostics.
Outstanding Sensitivity and Robust Performance
Leverage high sensitivity and strong inhibitor resistance to detect even scarce targets such as 10 fg of RNA or as few as 10 copies of RNA. SuperScript IV RT and Platinum Taq DNA Polymerase ensure fast, accurate, and reproducible amplification, making the kit suitable for demanding samples and complex experiments.
FAQ's of Invitrogen SuperScript IV One-Step RT-PCR System with Platinum Taq DNA Polymerase:
Q: How does the Invitrogen SuperScript IV One-Step RT-PCR System benefit my laboratory workflow?
A: By enabling reverse transcription and PCR amplification in a single tube, the system streamlines protocols, minimizes hands-on time, reduces contamination risk, and delivers reliable results rapidly-often in under one hour.Q: What types of RNA templates can be used with this kit?
A: The kit is suitable for total RNA, poly(A) mRNA, as well as viral or bacterial RNA, supporting a wide range of applications in both research and diagnostic setups.Q: When should DNase treatment be applied before using the kit?
A: DNase treatment is optional but can be performed to improve specificity by eliminating potential genomic DNA contamination, particularly when analyzing low abundance transcripts.Q: Where can I store the kit components and how long are they stable?
A: Store the kit at -20C. Under recommended conditions, components are stable for 6 months, and expiration details are indicated on the kit label. The enzymes tolerate multiple freeze-thaw cycles.Q: What process should be followed when setting up a reaction with this system?
A: Prepare a 25 L standard reaction by combining the reagent mix, enzyme, buffer, template RNA (10 fg to 1 g), and suitable primers. Run the protocol on a compatible thermal cycler, following the manual instructions provided.Q: How accurate and sensitive is the SuperScript IV One-Step RT-PCR System?
A: The system is highly accurate for gene expression analysis and can detect as few as 10 copies of target RNA, ensuring reliable quantitative and qualitative results even from challenging samples.Q: What are the main applications for this RT-PCR system?
A: This kit is designed for gene expression analysis, pathogen detection, and NGS library preparation, making it highly valuable for both research and diagnostic laboratories across India.Tell us about your requirement
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