Qiagen QIAamp DNA Mini QIAcube Kit (240)

Qiagen QIAamp DNA Mini QIAcube Kit (240)

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Product Details:

Usage Type Laboratory / Research Use
Accuracy High yield and purity (A260/A280 ratio: 1.71.9)
Shelf Life 12 months
Measurement Range Can process up to 100 l blood or 25 mg tissue per prep
Features High purity DNA extraction, automation compatible, ready-to-use reagents, fast processing
Function Automated DNA isolation and purification
Instruments Type Sample preparation kit for automated instruments
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Qiagen QIAamp DNA Mini QIAcube Kit (240) Price And Quantity

Qiagen QIAamp DNA Mini QIAcube Kit (240) Product Specifications

Real-Time Operation No
Measurement Range Can process up to 100 l blood or 25 mg tissue per prep
Shelf Life 12 months
Wall Mounted No
Material High-grade plastic, Silica-based membrane
Equipment Type DNA Purification Kit
Accuracy High yield and purity (A260/A280 ratio: 1.71.9)
Use Extraction and purification of genomic DNA
Portable No
Power Source Not Applicable (Used with QIAcube instrument)
Color White / Transparent (kit components)
Usage Type Laboratory / Research Use
Condition New
Operating Type Automated with QIAcube instrument
Storage Instructions Store at room temperature (1525C), observe kit manual for specific buffer storage
Technology Spin column and automated QIAcube technology
Function Automated DNA isolation and purification
Instruments Type Sample preparation kit for automated instruments
Features High purity DNA extraction, automation compatible, ready-to-use reagents, fast processing

Product Description

Brand Qiagen

Product Type DNA Extraction Kit

Pack Size 240T

CAT No 51326

Application Molecular Reagent

The QIAamp DNA Micro Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 L. DNA purified using the QIAamp DNA Micro Kit is free of proteins, nucleases and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCRand laser microdissection (LMD) PCR. Purified DNA may also be used in short-tandem repeat (STR) genotyping, single-nucleotide polymorphism (SNP) genotyping or pharmacogenomic research.

Automated High-Purity DNA Extraction

The QIAamp DNA Mini QIAcube Kit leverages advanced spin column and QIAcube automation technology to streamline DNA isolation, minimizing hands-on time and risk of contamination. Ready-to-use reagents and preassembled columns ensure consistent, high-purity yields suitable for sensitive genomic analysis. This automation caters to medium-to-high-throughput laboratories demanding reproducibility and reliability.

Versatile Sample Compatibility

Suitable for a variety of source materials, this kit efficiently processes whole blood, buffy coat, cultured cells, animal tissues, swabs, and bodily fluids. With high DNA recovery from up to 100 l of blood or 25 mg of tissue per sample, researchers can confidently use the kit across multiple projects without changing protocols or reagents.

Reliable Results for Downstream Applications

Extracted DNA achieves stringent purity standards (A260/A280 ratio: 1.7-1.9), supporting applications such as PCR, qPCR, genotyping, sequencing, Southern blotting, and RFLP. The optimized buffers and silica-based columns safeguard sample integrity, ensuring robust and reproducible results for demanding research environments.

FAQ's of Qiagen QIAamp DNA Mini QIAcube Kit (240):

Q: How does the QIAamp DNA Mini QIAcube Kit automate the DNA extraction process?

A: The kit is designed specifically for use with the QIAcube automated instrument, which handles all the manual steps of the spin column DNA purification process. This includes sample loading, lysis, DNA binding, washing, and elution, resulting in a streamlined workflow that reduces manual handling and increases reproducibility.

Q: What sample types can be processed using this kit?

A: The kit supports a wide variety of samples, including whole blood, buffy coat, up to 25 mg of animal tissues, cultured cells, swabs, and various body fluids. This versatility makes it ideal for labs working with diverse specimen types without modifying the protocol.

Q: When should I use the QIAamp DNA Mini QIAcube Kit?

A: Use this kit when you require high-yield, high-purity genomic DNA for downstream applications such as PCR, qPCR, genotyping, sequencing, or Southern blotting. It is particularly advantageous when processing multiple samples where automation will improve efficiency and reproducibility.

Q: Where should the kit be stored and how long is the shelf life?

A: Store the kit at room temperature between 15-25C, in accordance with the package insert instructions. Some buffers may require special storage; always consult the manual. The kit maintains optimal performance for up to 12 months from the date of manufacture.

Q: What is the typical processing time, and how many samples can be run per kit?

A: A complete run on the QIAcube instrument typically takes about 40 minutes per batch, depending on sample type. Each kit supports up to 240 DNA preparations, making it suitable for high-throughput settings.

Q: How much DNA can I expect to obtain, and what is the elution volume range?

A: Each spin column can bind up to 50 g of DNA per prep. The recommended elution volume ranges from 50 to 200 l, allowing flexibility based on your downstream application requirements.

Q: What are the main benefits of using this kit in molecular biology research?

A: This kit offers high yields and purity, minimizes human error through automation, is compatible with many sample types, and provides ready-to-use reagents. The resulting DNA meets stringent quality standards, ensuring suitability for a broad spectrum of genomic applications.

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