Qiagen QIAseq cfDNA All-in-One Kit (96)

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Product Details:

Instruments Type Consumables/Reagent kit
Accuracy High recovery and reproducibility for cfDNA extraction
Function Extraction and NGS-ready library preparation of cell-free DNA
Shelf Life Refer to product label (typically 12-18 months from manufacturing)
Measurement Range Input: 15 mL plasma per sample
Usage Type Laboratory/Research Use Only
Features Streamlined workflow for cfDNA extraction and NGS library construction; reduces hands-on time; high sensitivity and yield
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Qiagen QIAseq cfDNA All-in-One Kit (96) Price And Quantity

Qiagen QIAseq cfDNA All-in-One Kit (96) Product Specifications

Instruments Type Consumables/Reagent kit
Wall Mounted No
Equipment Type cfDNA Extraction and Library Preparation Kit
Technology Magnetic bead-based DNA purification & NGS library prep
Accuracy High recovery and reproducibility for cfDNA extraction
Real-Time Operation No
Function Extraction and NGS-ready library preparation of cell-free DNA
Measurement Range Input: 15 mL plasma per sample
Shelf Life Refer to product label (typically 12-18 months from manufacturing)
Operating Type Automatic / Semi-automatic (protocol dependent)
Usage Type Laboratory/Research Use Only
Material Reagents and Enzymes
Storage Instructions Store at 28C; some components may require -20C
Use Isolation and preparation of cell-free DNA (cfDNA) for NGS workflows
Portable No
Features Streamlined workflow for cfDNA extraction and NGS library construction; reduces hands-on time; high sensitivity and yield
Condition New
Color White reagent box (various reagent colors inside)

Product Description

Brand Qiagen

Product Type PCR Kit

Pack Size 96 Wells

CAT No 180025

Application Molecular Reagent

QIAseq cfDNA All-in-One Kits provide an optimized solution for cfDNA analysis using any NGS platform from Illumina or Ion Torrent. Kits enable optimal conversion of cfDNA at every step from plasma to NGS library and allow sensitive detection of even the rarest variants, providing you with the highest confidence in your results.

Streamlined cfDNA Workflow for NGS

The QIAseq cfDNA All-in-One Kit revolutionizes cfDNA extraction and library preparation by combining both procedures in a unified workflow. By reducing hands-on time and simplifying processes, laboratories can efficiently manage medium- to high-throughput sample loads, optimizing productivity for cancer research and prenatal applications.

Compatibility with Leading NGS Platforms

Engineered for broad compatibility, the kit enables seamless integration with Illumina and other major NGS systems. This makes it a versatile tool for laboratories aspiring to produce high-quality sequencing data from cfDNA, crucial for applications in liquid biopsy and translational research.

FAQ's of Qiagen QIAseq cfDNA All-in-One Kit (96):

Q: How does the Qiagen QIAseq cfDNA All-in-One Kit support high-throughput workflows?

A: The kit is optimized for medium- to high-throughput settings, allowing users to process up to 96 samples per run. Its integrated magnetic bead-based protocol streamlines both cfDNA extraction and NGS library preparation, reducing overall handling time to approximately 3-4 hours.

Q: What types of samples are suitable for use with this kit?

A: The QIAseq cfDNA All-in-One Kit is designed to process cell-free DNA from various liquid samples, including plasma, serum, and similar fluids. It effectively recovers cfDNA from 1-5 mL of plasma per sample.

Q: When should the reagents be stored at -20C versus 2-8C?

A: Most components should be stored at 2-8C. However, refer to the product label for specific storage instructions, as some reagents may require storage at -20C to maintain their stability and performance.

Q: Where can this kit be used?

A: The kit is intended for laboratory and research environments in institutions such as hospitals, academic labs, and biotechnology companies engaged in cfDNA research for oncology or prenatal diagnostics.

Q: What are the main benefits of using the QIAseq cfDNA All-in-One Kit in cancer research or liquid biopsy?

A: The kit offers high sensitivity and yield, which is critical for detecting low-abundance cfDNA in cancer or prenatal samples. Its reproducible results and seamless compatibility with NGS platforms make it reliable for translational and clinical research.

Q: Which NGS platforms are compatible with the libraries generated by this kit?

A: The libraries prepared using this kit are compatible with Illumina as well as other major next-generation sequencing platforms, giving users flexibility in their downstream sequencing choices.

Q: What is the shelf life of the QIAseq cfDNA All-in-One Kit?

A: The typical shelf life is 12-18 months from the date of manufacturing. It is important to check the product label for the exact expiry date and to follow all storage recommendations to ensure product integrity.

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