Qiagen MinElute 96 UF PCR Purification Kit (24)

Qiagen MinElute 96 UF PCR Purification Kit (24)

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Product Details:

Usage Type Laboratory molecular biology applications
Storage Instructions Store at room temperature (1525C), avoid direct sunlight
Function DNA purification after PCR amplification
Features Ultra-fast PCR purification, high-yield, 96-well plate, suitable for automation
Instruments Type Nucleic acid purification system accessory
Shelf Life 12 months
Measurement Range PCR products from 70 bp to 4 kb
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Qiagen MinElute 96 UF PCR Purification Kit (24) Price And Quantity

Qiagen MinElute 96 UF PCR Purification Kit (24) Product Specifications

Features Ultra-fast PCR purification, high-yield, 96-well plate, suitable for automation
Power Source Not required (manual process)
Use PCR product purification and cleanup
Condition New
Function DNA purification after PCR amplification
Storage Instructions Store at room temperature (1525C), avoid direct sunlight
Material High-quality plastic and proprietary purification membrane
Color White/transparent (components)
Usage Type Laboratory molecular biology applications
Equipment Type PCR Purification Kit
Accuracy High DNA recovery efficiency (>80%)
Measurement Range PCR products from 70 bp to 4 kb
Weight Approx. 500g per kit
Shelf Life 12 months
Wall Mounted No
Instruments Type Nucleic acid purification system accessory
Technology Silica membrane technology
Portable Yes
Real-Time Operation No
Operating Type Manual, 96-well plate format
Dimension (L*W*H) Standard 96-well plate size (approx. 12.8 x 8.6 x 1.6 cm)
Noise Level Silent operation

Safety Information Follow standard laboratory safety procedures
Throughput 96 samples per run
Automation Compatibility Compatible with liquid handling systems
Sample Volume 10100 L
Intended User For research use only, not for diagnostic use
Cat No 28051 (24 plate kit)
Processing Time < 30 minutes for 96 reactions
Binding Capacity per Well Up to 5 g DNA
Application Removal of primers, nucleotides, enzymes, and salts from PCR reactions
Elution Volume Minimum 10 L
Kit Contents MinElute 96 UF Plate, Collection microtubes, buffers, protocol booklet
Lot Number Provided on product packaging

Product Description

Brand Qiagen

Product Type PCR Kits

Pack Size 24T

CAT No 28053

Application Molecular Reagent

The MinElute 96 UF PCR Purification Kit provides 96-well plates for high-throughput ultrafiltration-based purification of PCR products 100 bp in size. The plates are designed to allow elution in very small volumes (as little as 20 l) delivering high yields of highly concentrated DNA. The procedure can be automated on the BioRobot Universal System. DNA fragments purified with the MinElute system are ready for direct use in many applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription. The MinElute 96 UF PCR Purification Kit requires the use of the QIAvac Multiwell vacuum manifold (cat. no. 9014579).

High-Throughput Efficiency

Process up to 96 PCR samples in a single run with minimal hands-on time. The MinElute 96 UF PCR Purification Kit is ideal for laboratories looking to increase throughput without compromising on DNA purity or yield, thanks to its user-friendly plate-based design and fast processing workflow.

Superior DNA Recovery and Purity

Leveraging silica membrane technology, this kit achieves high DNA recovery rates (>80%) and removes impurities such as salts, enzymes, and primers from amplified products. The result is a highly purified DNA sample, optimized for downstream molecular biology applications.

Automation and Versatility

Designed to fit seamlessly into automated laboratory systems, the MinElute 96 UF kit is compatible with most liquid handling instruments. It is also suited to manual operation, making it a versatile choice for diverse research environments and sample throughput requirements.

FAQ's of Qiagen MinElute 96 UF PCR Purification Kit (24):

Q: How does the Qiagen MinElute 96 UF PCR Purification Kit improve DNA cleanup after PCR?

A: This kit uses silica membrane technology within a 96-well plate to efficiently bind and purify DNA, rapidly removing residual primers, nucleotides, enzymes, and salts from PCR reactions, resulting in highly purified DNA suitable for downstream applications.

Q: What is the maximum DNA binding capacity per well in this kit?

A: Each well on the MinElute 96 UF Plate can bind up to 5 g of DNA, allowing for efficient purification from a variety of PCR product sizes and concentrations.

Q: When should I use this kit in my workflow?

A: Use the MinElute 96 UF PCR Purification Kit immediately following PCR amplification to quickly and effectively clean up your DNA products before downstream molecular analyses such as sequencing, cloning, or qPCR.

Q: Where can the kit be used, and is it compatible with automated systems?

A: The kit is designed for laboratory use in molecular biology research and is compatible with both manual and automated (liquid handling) systems, making it suitable for high-throughput and automated workflows.

Q: What is the process for purifying DNA with this kit?

A: Add the PCR reaction to the MinElute 96 UF Plate with the supplied buffers, allow DNA to bind the membrane, wash away impurities, and finally elute the purified DNA in as little as 10 L. The entire process takes less than 30 minutes for 96 samples.

Q: How does using this kit benefit my laboratory operations?

A: The kit offers ultra-fast processing times, high DNA recovery efficiency, and compatibility with automation, which collectively increase throughput, ensure consistent sample quality, and save valuable laboratory time.

Q: What are the storage and handling instructions for the Qiagen MinElute 96 UF PCR Purification Kit?

A: Store the kit at room temperature (15-25C), avoid direct sunlight, and follow standard laboratory safety guidelines. Ensure components are kept in optimal condition for up to 12 months shelf life.

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