Qiagen Ni-NTA Superflow

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Product Details:

Function Selective binding and elution of His-tag proteins
Features High capacity, low metal ion leakage, reusable
Instruments Type Chromatography material
Shelf Life 2 years from production
Usage Type Laboratory
Storage Instructions Store at 28C, do not freeze
Equipment Type Chromatography Resin
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Qiagen Ni-NTA Superflow Price And Quantity

Qiagen Ni-NTA Superflow Product Specifications

Use Purification of histidine-tagged proteins
Technology Affinity Chromatography
Function Selective binding and elution of His-tag proteins
Color Beige/Yellowish (Resin appearance)
Wall Mounted No
Features High capacity, low metal ion leakage, reusable
Material Agarose, chelating Ni-NTA (nickel-nitrilotriacetic acid)
Real-Time Operation No
Instruments Type Chromatography material
Portable Yes
Operating Type Manual/Automated compatible
Condition New
Dimension (L*W*H) Varies by packaging (e.g., bottle or column)
Equipment Type Chromatography Resin
Shelf Life 2 years from production
Storage Instructions Store at 28C, do not freeze
Weight Varies by Product Size
Usage Type Laboratory

Product Description

Brand Qiagen

Product Type PCR Kits

Pack Size 500 mL

CAT No 30250

Application Molecular Reagent

Ni-NTA Agarose is an affinity chromatography matrix for purifying recombinant proteins carrying a His tag. Histidine residues in the His tag bind to the vacant positions in the coordination sphere of the immobilized nickel ions with high specificity and affinity. Cleared cell lysates are loaded onto the matrices. His-tagged proteins are bound, and other proteins pass through the matrix. After washing, His-tagged proteins are eluted in buffer under native or denaturing conditions.

Consistent High Efficiency for Protein Purification

Designed for selective binding and elution of histidine-tagged proteins, Qiagen Ni-NTA Superflow ensures efficient capture, high yield, and purity. Its 40 mg/ml resin binding capacity delivers impressive throughput for research and industrial laboratories. The resin can be reused multiple times, making it cost-effective for frequent purification cycles, and supports both denaturing and native conditions.

Robust Structure for Flexibility and Reproducibility

Constructed of 6% cross-linked agarose with bead sizes of 60160 m, this resin offers durability and stability across a wide pH range (214 short term; 48 long term). Its robust particle structure is compatible with pressures up to 0.3 MPa (3 bar), and chemical resistance allows use with standard buffers, denaturants, reducing agents, and 20% ethanol, ensuring versatile laboratory applications.

Easy Handling and Reliable Storage

Qiagen Ni-NTA Superflow arrives as a ready-to-use slurry in 20% ethanol, with recommended storage at 28C (do not freeze) for optimal shelf life of up to two years from production. Available in various quantities and packaging, it accommodates diverse usage requirements. The resins beige/yellowish color provides a visual reference for quality assessment before and during experiments.

FAQs of Qiagen Ni-NTA Superflow:

Q: How is Qiagen Ni-NTA Superflow used for purifying His-tagged proteins?

A: To purify His-tagged proteins with Qiagen Ni-NTA Superflow, you simply load your sample onto a column packed with the resin. The resins Ni-NTA groups selectively bind histidine tags, allowing non-specific proteins to be washed away. Elution with imidazole or similar agents releases the purified His-tagged protein for further analysis or applications.

Q: What are the recommended storage conditions for Ni-NTA Superflow resin?

A: Store the resin at 28C in its original slurry containing 20% ethanol to prevent microbial growth. Do not freeze the resin as this may damage the agarose bead structure and affect its performance.

Q: When should the resin be regenerated and how many times can it be reused?

A: Regenerate the resin after each purification cycle using standard Ni-NTA cleaning procedures. The resin can typically be reused multiple times if properly cleaned and inspected for performance, maximizing cost efficiency for frequent users.

Q: Where can I obtain different packaging sizes of this resin?

A: Qiagen offers Ni-NTA Superflow in various bottle and column formats. Refer to Qiagens catalog or authorized dealers, distributors, and suppliers in India to select and purchase the appropriate package for your requirements.

Q: What types of buffers and conditions are compatible with this resin?

A: Ni-NTA Superflow is chemically compatible with most commonly used laboratory buffers, including those containing up to 20% ethanol, denaturants, and reducing agents. Always check the buffer composition for compatibility with nickel-affinity chromatography protocols.

Q: What are the main benefits of using Ni-NTA Superflow in protein purification?

A: The resin delivers high binding capacity, low metal ion leakage, chemical stability, and supports high purity yields. It is suitable for both manual and automated systems and contributes to cost savings by allowing multiple regeneration cycles.

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