BioRad Quick Start Bradford Protein Assay Kit 4

BioRad Quick Start Bradford Protein Assay Kit 4

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Product Details:

Usage Type Laboratory
Instruments Type Biochemistry reagent kit
Shelf Life 12 months
Storage Instructions Store at 28C
Measurement Range 112 mg/mL (recommended for BSA standards)
Function Quantification of total protein
Features Rapid, sensitive protein assay, minimal preparation required
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BioRad Quick Start Bradford Protein Assay Kit 4 Price And Quantity

BioRad Quick Start Bradford Protein Assay Kit 4 Product Specifications

Wall Mounted No
Function Quantification of total protein
Condition New
Equipment Type Protein Assay Kit
Use Protein Quantification
Operating Type Manual
Features Rapid, sensitive protein assay, minimal preparation required
Weight Approx. 4 x 5 mL bottles
Technology Bradford dye binding method
Instruments Type Biochemistry reagent kit
Portable Yes
Real-Time Operation No
Usage Type Laboratory
Color Blue (reagent color)
Measurement Range 112 mg/mL (recommended for BSA standards)
Storage Instructions Store at 28C
Material Reagent Kit
Shelf Life 12 months

Protocol Format Compatible with microplate, test tube, and cuvette formats
Catalog Number 500-0205
Limit of Detection Approximately 1 g/mL (with microplate protocol)
Assay Time 510 minutes
Application Area Molecular biology, biochemistry, protein analysis
Standard Curve Recommended using bovine serum albumin (BSA)
Detection Method Colorimetric (absorbance at 595 nm)
Compatible Sample Types Suitable for cell lysates, purified proteins, and a wide range of biological samples
Hazard Information Non-hazardous
Kit Contents Four 5 mL bottles of Bradford reagent, sufficient for up to 200 standard assays

Product Description

Brand BioRad

Product Type Protein Assay Kit

Pack Size 1 L

CAT No 5000204

Application Molecular Biology

The Quick Start Bradford protein assay is a simple and accurate procedure for determining the concentration of protein in solution. The assay supplies ready-to-use dye reagent at 1x concentration and two protein standards at seven prediluted concentrations (0.125, 0.25, 0.5, 0.75, 1.0, 1.5, and 2.0 mg/ml). Protein concentration is determined in one step, with no need to dilute standards. Quick Start Bradford protein assay kits offer either bovine serum albumin or bovine I3-globulin standard sets.

Fast and Sensitive Protein Quantification

Obtain accurate protein concentration measurements in as little as 5-10 minutes using the Bradford dye-binding method. The kit features a highly sensitive colorimetric assay with a detection limit as low as 1 g/mL, making it perfect for a wide range of laboratory protein analyses. Its rapid protocol streamlines your workflow without compromising reliability.

Versatile Sample Compatibility and Flexible Formats

Designed to work with diverse biological samples, including cell lysates and purified proteins, this kit adapts to your laboratory's needs. Whether you use microplates for high-throughput screening or prefer cuvette or test tube formats for individual samples, the assay delivers consistent results across platforms.

Easy Storage and Extended Shelf Life

This reagent kit remains stable for up to 12 months when stored at 2-8C, ensuring long-term availability and convenience. Its non-hazardous formulation also adds an extra layer of safety for daily use in any laboratory setting.

FAQ's of BioRad Quick Start Bradford Protein Assay Kit 4:

Q: How do I use the Bio-Rad Quick Start Bradford Protein Assay Kit 4?

A: Simply mix your sample and Bradford reagent according to the protocol, incubate for 5-10 minutes, and measure absorbance at 595 nm using a compatible instrument (microplate reader, spectrophotometer, or cuvette).

Q: What types of samples are suitable for this assay kit?

A: The kit is compatible with cell lysates, purified proteins, and a broad range of biological samples, making it suitable for most protein quantification needs in molecular biology and biochemistry.

Q: When should I use the recommended BSA standard curve?

A: A BSA (bovine serum albumin) standard curve is recommended for best accuracy and consistency, especially when quantifying unknown protein samples. Prepare the curve alongside your samples in each assay run.

Q: Where should I store the Bradford reagent to maintain stability?

A: Store all reagent bottles at 2-8C (refrigerated). This ensures optimal reagent performance and extends shelf life up to 12 months.

Q: What is the measurement range and detection limit for this kit?

A: The recommended measurement range is 1-12 mg/mL for BSA standards, with a detection limit of approximately 1 g/mL when following the microplate protocol.

Q: What are the benefits of using this particular protein assay kit?

A: The kit offers a rapid, sensitive, and straightforward method for protein quantification, requiring minimal preparation and supporting multiple assay formats to maximize laboratory efficiency.

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